Road-traffic accidents--a demographic and topographic analysis.

Injuries and fatalities occur in all forms of transportation, but numerically, road-traffic accidents account for the great majority worldwide. There is little that the autopsy surgeon can contribute to the elucidation of factors leading to the accident as it is largely the circumstantial and forensic laboratory evidence which is likely to reveal a non-accidental cause. However, the doctor's role in detecting the compatibility/incompatibility of the injuries with those usually sustained in traffic accidents (to detect any which are 'atypical', e.g. focal depressed fracture of the skull), distinguishing antemortem from postmortem injuries, demonstrating the presence of any disease capable of creating sudden incapacity and analysing samples for alcohol/drugs, etc., can go a long way in assigning roles to the human and to some extent vehicular and environmental factors. This warrants that a meticulous autopsy be conducted and not merely a catalogue of injuries. It must be appreciated that a fatal accident is likely to result in litigation and the extent of litigation cannot be anticipated at the time of the autopsy. One must, therefore, aim at the close study of any accident victim and a careful assessment of the case is always rewarding. The present study was undertaken in the Department of Forensic Medicine at (a) Government Medical College, Jammu (1991-93), (b) Mulana Azad Medical College, New Delhi (1993-95) and (c) Government Medical College, Chandigarh (1994-June 2000), with the object of doing a comparative analysis of the various aspects of the road-traffic accidents and accidental deaths in three topographically and demographically different cities in India and to suggest remedial measures to bring down the accident rate.

Heart disease vis-à-vis trauma.

Virtually all forensic experts deal not only with criminal, suspicious, accidental and suicidal deaths, but are also confronted with a wide range of deaths where a significant natural element is revealed at autopsy. The assaulted victim that dies suddenly or unexpectedly from a stroke during or immediately succeeding the receipt of some non-fatal injuries, or otherwise suffers a clinically unexplained death, can pose far greater difficulties over causation than a gun-shot or a stabbing. This paper presents an analysis of the problem and an approach for determining the cause of death in cases of concurrent trauma with heart disease, and in cases with a substantial natural element of disease but exclusion of trauma. Relevant cases with history, autopsy findings, histopathological findings and toxicological findings are presented in order to illustrate the issue from a practical angle.

Learning-dependent synaptic modifications in the cerebellar cortex of the adult rat persist for at least four weeks.

Several experiments have demonstrated increased synapse number within the cerebellar cortex in association with motor skill learning but not with motor activity alone. The persistence of these synaptic changes in the absence of continued training was examined in the present experiment. Adult female rats were randomly allocated to either an acrobatic condition (AC) or a motor activity condition (MC). The AC animals were trained to traverse a complex series of obstacles, and each AC animal was pair-matched with an MC animal that traversed an obstacle-free runway. These animals were further assigned to one of three training conditions. Animals in the EARLY condition were trained for 10 consecutive days before being killed, animals in the DELAY, condition received the same 10 d of training followed by a 28 d period without training, and animals in the CONTINUOUS condition were trained for the entire 38 d. Unbiased stereological techniques were used to obtain estimates of the number of synapses per Purkinje cell within the cerebellar paramedian lobule. Results showed the AC animals to have significantly more synapses per Purkinje cell than the MC animals in all three training conditions. There were no differences in the number of synapses per Purkinje cell among the EARLY, DELAY, and CONTINUOUS conditions. These data demonstrate that both the motor skills and the increases in synapse number presumed to support them persist in the absence of continued training.

Three-dimensional spectral-spatial EPR imaging of free radicals in the heart: a technique for imaging tissue metabolism and oxygenation.

It has been hypothesized that free radical metabolism and oxygenation in living organs and tissues such as the heart may vary over the spatially defined tissue structure. In an effort to study these spatially defined differences, we have developed electron paramagnetic resonance imaging instrumentation enabling the performance of three-dimensional spectral-spatial images of free radicals infused into the heart and large vessels. Using this instrumentation, high-quality three-dimensional spectral-spatial images of isolated perfused rat hearts and rabbit aortas are obtained. In the isolated aorta, it is shown that spatially and spectrally accurate images of the vessel lumen and wall could be obtained in this living vascular tissue. In the isolated rat heart, imaging experiments were performed to determine the kinetics of radical clearance at different spatial locations within the heart during myocardial ischemia. The kinetic data show the existence of regional and transmural differences in myocardial free radical clearance. It is further demonstrated that EPR imaging can be used to noninvasively measure spatially localized oxygen concentrations in the heart. Thus, the technique of spectral-spatial EPR imaging is shown to be a powerful tool in providing spatial information regarding the free radical distribution, metabolism, and tissue oxygenation in living biological organs and tissues.