ABSTRACT
Background: Melanoma is an aggressive malignant tumor with high metastatic potential. FNAC provides a quick and effective tool to diagnose primary and metastatic melanoma cases. Aims and Objectives: To document the epidemiological features of malignant melanoma cases attending in a tertiary care centre in India, with special reference to different cytomorphological parameters. Materials and Methods: This was a retrospective hospital based study done in the Department of Pathology. 39 cases of malignant melanoma diagnosed on cytology during a period of 3 years were retrieved, reviewed and studied. Results: Out of the 39 cases,21 were males(1.16 male:female ratio). The mean age was 53 years. Metastatic cases were more common than primary melanoma cases. Most common site for metastasis was liver followed by inguinal lymph node. Epitheloid was the most common pattern. Conclusion: Characterstic melanin pigment although common, is not always present. Thus, cytopathologists must know the various cytomorphological spectrum of features of melanoma.
ABSTRACT
The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to identify the revert mutations which are present in strains, it can also be used to detect the mutagenicity of environmental samples such as drugs, dyes, reagents, cosmetics, waste water, pesticides and other substances which are easily solubilized in a liquid suspension. We present the protocol for conducting Ames test in the laboratory.
