ABSTRACT
For rapid and successful treatment of infectious diseases, detection of the presence of microorganisms is essential. Traditional culture-based approaches are limiting and time consuming for microbial identification. The most popular staining technique for identifying Gram-positive and Gram-negative microorganisms in various tissues is called Gram staining. This method is utilized in both clinical practice and research. Gram staining of the oral smears is the preliminary step in the identification of any pathological shift in normal oral microbiota. This review discusses the principle of gram stain emphasizing its significance in diagnostic utility for oral smears.
ABSTRACT
OBJECTIVE: To evaluate the anti-carcinogenic effect of Centella Asiatica on to evaluate the Anti-Carcinogenic Effect of Centella Asiatica on Oral Cancer Cell Line oral cancer cell line. MATERIALS AND METHODS: Oral Cancer cell line and normal oral keratinocyte cell line were procured.Centella asiatica extract was prepared. The cells were then subjected to the test herbal specimens -Centella asiatica extract in succeeding concentrations of 25 µg/ml, 50 µg/ml, 100 µg/ml at time intervals of 24,48 and 72 hours. Cisplatin (2 µg/ml, 4 µg/ml, 6 µg/ml, 8 µg/ml) was used as a positive control. This experiment was done in triplets. RESULTS: The study revealed that the p values were less than 0.05 at concentration 12.5µg/ml, 25µg/ml, 50 µg/ml,100 µg/ml and time period of 24hrs,48hrs,72hrs, thus implying that at these concentrations and time period, the obtained data were statistically significant, thus indicating that there is a statistically significantly decreases in the viable cells as the concentration of the drug as a time period increases The results reveals that centella asiatica possess potential effect of anti-carcinogenic, effect when compared to positive control (Cisplatin). CONCLUSION: The current study reveals that Centella asiatica has an potential anti-carcinogenic effect on oral cancer cell line. So this can be used to treat oral cancer with minimal crippling as compared with allopathic drugs.
