Implications of noncoding regulatory functions in the development of insulinomas.

Insulinomas are rare neuroendocrine tumors arising from pancreatic ß cells, characterized by aberrant proliferation and altered insulin secretion, leading to glucose homeostasis failure. With the aim of uncovering the role of noncoding regulatory regions and their aberrations in the development of these tumors, we coupled epigenetic and transcriptome profiling with whole-genome sequencing. As a result, we unraveled somatic mutations associated with changes in regulatory functions. Critically, these regions impact insulin secretion, tumor development, and epigenetic modifying genes, including polycomb complex components. Chromatin remodeling is apparent in insulinoma-selective domains shared across patients, containing a specific set of regulatory sequences dominated by the SOX17 binding motif. Moreover, many of these regions are H3K27me3 repressed in ß cells, suggesting that tumoral transition involves derepression of polycomb-targeted domains. Our work provides a compendium of aberrant cis-regulatory elements affecting the function and fate of ß cells in their progression to insulinomas and a framework to identify coding and noncoding driver mutations.

Reliability of a handball specific strength test battery and the association with sprint and throwing performance in young handball players.

BACKGROUND: A greater motivation for coaches to be more involved in assessment could be the development of specific strength tests closer to specific handball tasks. Therefore, the aims were to determine the reliability of a handball specific strength test battery in young handball players using a functional electromechanical dynamometer (FEMD) and to evaluate the association between the strength test battery and performance measurements. METHODS: Thirty-two young handball players (25% males and 75% females) performed a repeated-measurement design over four weeks. The players conducted one session per week, with each measurement consisting of two isometric tests to obtain isometric peak force, and four incremental tests. Moreover, performance handball tests (sprint and throwing velocity [TV]) were recorded. RESULTS: The reliability of the whole handball specific strength tests was very high for the peak and mean strength (ICC=0.70-0.85; CV=8.49-13.99; SEM=0.44-0.67 kg). The step forward had a moderate association with 5 to 10 meters' time. The highest correlation between TVmean was found with the peak strength of standing lift and between TVpeak with peak strength of unilateral throw. The sprint total time could be explained by Body Mass Index and peak strength of the standing lift in the 40% and the TVpeak could be explained by arm span and mean strength of the standing lift in the 68%. CONCLUSIONS: The high reliability for all exercises and the significant correlation with handball performance (sprint time and TV) allow us to include these tests in handball team evaluation and training.

Los Músculos Isquiosurales y su Capacidad Profiláctica sobre la Translación Tibial Anterior en Lesión de Ligamento Cruzado Anterior: Una Revisión Sistemática / The Hamstrings Muscles and their Prophylactic Capacity on Anterior Tibial Translation in Anterior Cruciate Ligament Injury: A Systematic Review

La ruptura de ligamento cruzado anterior (LCA) es la lesión de rodilla más común que se trata de una reconstruc- ción quirúrgica. El objetivo principal de esta revisión fue analizar la importancia de la función de los músculos isquiosurales como factor de riesgo de posibles lesiones de LCA. Se llevó a cabo siguiendo las normas Preferred Reported Item for Systematic Reviews and Meta- analyses (PRISMA). La fuente de la recopilación de datos fue la consulta directa de las bases de datos Pubmed, Sportdiscus, Web of Science y Scopus. Para la recuperación documental, se utilizaron varias palabras clave y se evaluó la calidad de los estudios que fueron seleccionados mediante la escala PEDro. Los déficits de la función muscular en los músculos isquiosurales se han relacionado con una mayor translación tibial anterior y, como consecuencia, un incremento del estrés tensional sobre el LCA entre los 10° y 45° de flexión de rodilla. Una co-activación de los músculos isquiosurales con el músculo cuádriceps femoral puede ser de gran ayuda para reducir los factores de riesgo de la lesión LCA.

SUMMARY: Anterior cruciate ligament (ACL) rupture is the most common knee injury to undergo surgery in the sports setting. The main objective of this review was to analyze the importance of hamstring function as a risk factor for potential ACL injury. It was conducted following the Preferred Reported Item for Systematic Reviews and Meta-analyses (PRISMA) guidelines. The source of data collection was direct consultation of the Pubmed, Sportdiscus, Web of Science and Scopus databases. For documentary retrieval, several key words were used and the quality of the studies that were selected was assessed using the PEDro scale. Muscle function deficits in the hamstrings have been related to increased anterior tibial translation and, as a consequence, increased tensional stress on the ACL between 10° and 45° of knee flexion. A co-activation of the hamstrings with the quadriceps may be helpful in reducing the risk factors for ACL injury.

The Most Common Handball Injuries: A Systematic Review.

Handball is a team sport involving a great physical demand from its practitioners in which a high number of injuries occur, affecting individual and collective performance. Knowledge of the injuries is of great importance for their prevention. The objective of the present study was to identify, locate and compare the most frequent injuries and injury mechanisms in handball practice. It was carried out following the Preferred Informed Item for Systematic Reviews and Meta-analysis (PRISMA) guidelines. The source of data collection was direct consultation of the PubMed and Medline databases. Several keywords were used for the documentary retrieval, and the quality of the studies that were selected was evaluated. Of the 707 studies retrieved, only 27 were considered appropriate for the review, and quality scores were obtained that ranged from 10 to 26 points, out of a maximum of 28. The most frequent injuries in handball players are located in the lower limbs (thigh, knee and ankle), and in the shoulder in the upper limbs. Regarding the playing position, the players who play over the 6-m line are the most affected by injuries, while the women players have a higher probability of injury. Most injuries occur during competition.

Efecto de la edad relativa en la élite del balonmano femenino español y su relación con aspectos antropométricos, de rendimiento y velocidad de lanzamiento / Efeito relativo da idade na élite do andebol feminino espanhol e sua relação com aspectos antropométricos, desempenho e velocidade de arremesso / Relative age effect in Spanish elite of female handball and its relationship with anthropometry, physical performance and throwing speed

El efecto de la edad relativa(RAE)es un aspecto de relevancia en el deporte en general y en balonmano en particular. Ha sido objeto de gran cantidad de estudios centrados mayoritariamente en deporte masculino. Los estudios sobre RAEen balonmano femenino, no son tan numerosos, y no aclaran el efecto de la misma en esta modalidad deportiva. Por ello, el objetivo de este estudio fue determinar la existencia deRAEen jugadoras de élite de balonmano, así como valorar dicho efecto en función de variables antropométricas, de condición física y técnico tácticas (velocidad de lanzamiento). Han sido evaluadas 136 jugadoras de balonmano de los ocho primeros equipos de división de honor. Se ha encontrado un RAE(con la prueba de chi-cuadrado) en las jugadoras de balonmano, sustentado por aquellas que formaron parte de los equipos nacionales. No se apreció RAEen las variables antropométricas, ni en las velocidades de lanzamiento con y sin interacción. La lateralidad y el Squat Jump, presentaron RAE. El RAEes importante para llegar a la selección, pero para jugar en la máxima categoría el efecto de la edad relativa no es tan importante. Son necesarios más estudios sobre el RAEen el balonmano femenino. (AU)

Relative age effect(RAE)is an aspect of relevance in sport in general and in handball in particular. It has been the subject of a great number of studies focused largely on men's sport. RAE studies in female handball are not so numerous, and do not clarify the effect of female handball on this sport. Therefore, the aim of this study was to establish the effect of RAEon élite handball players, as well as to assess this effect based on anthropometric variables, physical fitness and technical and tactical skills (throwing speed). 136 handball players from the first eight honour division teams have been assessed. RAE has been found (with chi-square test) in the handball players supported by those included in national teams. No RAEwere found on anthropometric variables, nor on throwing speed with and without interaction. Laterality and Squat Juam presented RAE. RAEis important to reach the national team, but to play in the highest category the effect of relative age seems to lose weight Further studies on the effect of RAEon women's handball are needed. (AU)

O efeito da idade relativa(RAE)é um aspecto de relevância no desporto em geral e no andebol em particular. Tem sido objeto de elevado número de estudos centrados em grande parte no andebol masculino. Os estudos sobre RAEno andebol feminino, não são tão numerosos, e não clarejam o efeito da mesma nesta modalidade esportiva. Por isso, o objetivo deste estudo foi determinar a existência do RAEem jogadoras de élite de andebol, assim como valorar este efeito em função das variáveis antropométricas, de condição física e técnico-táticas (velocidade de remate). Foram avaliadas 136 jogadoras de andebol das oito primeiras equipas da divisão de honra. Encontrou-se um RAE(foi usado o teste qui-quadrado) nas jogadoras de andebol sustentado pelas que participam nas seleções nacionais. Não se encontrou RAEnas variáveis antropométricas, nem na velocidade de remate com e sem interação. A lateralidade e o Squat Juam apresentaram RAE. O RAEé importante para chegar às seleções, mas para jogar na categoria máxima o RAEparece perder significado. São necessários mais estudos sobre o RAEno andebol feminino. (AU)

A Training Proposal to Improve Multidirectional Running Technique in Male and Female Handball Players: A Pilot Study.

Multidirectional running has been described as an important factor in team sports performance. The aim of the present study was to determine changes in T-test, 505 time, 10 m sprint, squat jump (SJ), countermovement jump (CMJ), countermovement jump right leg (CMJRL), and countermovement jump left leg (CMJLL) following exposure to 12 sessions over 4 weeks of a multidirectional running sprint training intervention in male and female handball players. A total of 31 handball players (15 male and 16 female) were recruited for this study and then randomly assigned to an experimental group (EG) or control group (CG). Male EG players showed improvements in 505 Preferred Side (PS) (p ≤ 0.05), 505 Non-Preferred Side (NPS) (p ≤ 0.05), and 10 m sprint (p ≤ 0.05), while female EG players presented statistically significant improvements between pre- and post-test for the T-test (p ≤ 0.05), 505 PS (p ≤ 0.05), 505 NPS (p ≤ 0.05), and 10 m sprint (p ≤ 0.05). No statistically significant pre- and post-test differences were observed in CG (all p ≥ 0.05) or between male and female players. We found an improvement in handball players' agility and speed of movement following the intervention protocol, suggesting the need to introduce this program into our training sessions. It may also be necessary to select and develop more specific tests in order to evaluate multidirectional work in handball players.

High-Performance Handball Player's Time-Motion Analysis by Playing Positions.

The purpose of this study was to analyze the on-court demands of handball players during the European Handball Federation Champions League Final Four (VELUX EHF FINAL4) 2019 to define time-motion characteristics (played time; covered distances) both in offense and defense. Furthermore; we aimed to define position-specific demands and differences among them. Forty players from three teams were analyzed during the tournament using a local positioning system (LPS) for the first time in top handball. Players covered similar distances both in offense (1388.28 ± 2627.08 m), and in defense (1305.47 ± 5059.64 m) and remained on court for a similar average time (15.69 ± 8.02 min and 15.40 ± 8.94 min respectively). When locomotion activities were normalized according to the time they spent on court; significant differences were found for defense compared to offense in walking (+20%; p < 0.000; Cohen's effect size (ES) = 1.01) and jogging (-29.6%; p = 0.000; ES = 0.90), as well as a tendency for high-intensity running (+ 25.2%; p = 0.077; ES = 0.31). Per playing position; center and left back (CB = 94.86 ± 10.98 m·min-1; LB = 96.55 ± 24.65 m·min-1) showed the highest running pace in offense and mid-left; front center defender and outside right for the defense (ML = 90.38 ± 30.16 m·min-1; FCD = 87.04 ± 14.94 m·min-1; OR = 89.64 ± 34.93 m·min-1). In conclusion; profile differences existed among players' position activity; both in offense and defense; which should be taken into account when designing specific physical training programs.

An integrated multi-omics approach identifies the landscape of interferon-α-mediated responses of human pancreatic beta cells.

Interferon-α (IFNα), a type I interferon, is expressed in the islets of type 1 diabetic individuals, and its expression and signaling are regulated by T1D genetic risk variants and viral infections associated with T1D. We presently characterize human beta cell responses to IFNα by combining ATAC-seq, RNA-seq and proteomics assays. The initial response to IFNα is characterized by chromatin remodeling, followed by changes in transcriptional and translational regulation. IFNα induces changes in alternative splicing (AS) and first exon usage, increasing the diversity of transcripts expressed by the beta cells. This, combined with changes observed on protein modification/degradation, ER stress and MHC class I, may expand antigens presented by beta cells to the immune system. Beta cells also up-regulate the checkpoint proteins PDL1 and HLA-E that may exert a protective role against the autoimmune assault. Data mining of the present multi-omics analysis identifies two compound classes that antagonize IFNα effects on human beta cells.

The impact of proinflammatory cytokines on the ß-cell regulatory landscape provides insights into the genetics of type 1 diabetes.

The early stages of type 1 diabetes (T1D) are characterized by local autoimmune inflammation and progressive loss of insulin-producing pancreatic ß cells. Here we show that exposure to proinflammatory cytokines reveals a marked plasticity of the ß-cell regulatory landscape. We expand the repertoire of human islet regulatory elements by mapping stimulus-responsive enhancers linked to changes in the ß-cell transcriptome, proteome and three-dimensional chromatin structure. Our data indicate that the ß-cell response to cytokines is mediated by the induction of new regulatory regions as well as the activation of primed regulatory elements prebound by islet-specific transcription factors. We find that T1D-associated loci are enriched with newly mapped cis-regulatory regions and identify T1D-associated variants disrupting cytokine-responsive enhancer activity in human ß cells. Our study illustrates how ß cells respond to a proinflammatory environment and implicate a role for stimulus response islet enhancers in T1D.

Influence of Physical Aspects and Throwing Velocity in Opposition Situations in Top-Elite and Elite Female Handball Players.

The relationship between anthropometric and physical characteristics of female handball players and throwing velocity is considered an important factor in handball performance. The aim of this study was to examine key differences in anthropometric and fitness characteristics between top elite and elite female players competing in the first Spanish handball league. A total of 89 players from the first Spanish handball league were divided into two groups, top elite (38) and elite (51) players, and assessed for anthropometric and fitness characteristics (throwing velocity, vertical jump and hand grip strength). A Student's t-test was used to determine whether a statistically significant difference between the two different levels of play occurred. Significant differences were found in age, body height, body mass, arm span, muscle mass, different girths (tensed and flexed arm, forearm, wrist, ankle), dominant hand width and length, different breadths (biacromial, bitrochanteric, bistyloid breadth and biepicondylar humerus) and fitness characteristics (hand grip strength and throwing velocity for different positions). Top elite players compared to elite players showed differences in anthropometric characteristics which were mainly located in the upper limbs and mostly were nonmodifiable aspects by training. Furthermore, the best players were able to maintain a high throwing velocity in different tactical situations.

Assay for Transposase Accessible Chromatin (ATAC-Seq) to Chart the Open Chromatin Landscape of Human Pancreatic Islets.

The regulatory mechanisms that ensure an accurate control of gene transcription are central to cellular function, development and disease. Such mechanisms rely largely on noncoding regulatory sequences that allow the establishment and maintenance of cell identity and tissue-specific cellular functions.The study of chromatin structure and nucleosome positioning allowed revealing transcription factor accessible genomic sites with regulatory potential, facilitating the comprehension of tissue-specific cis-regulatory networks. Recently a new technique coupled with high-throughput sequencing named Assay for Transposase Accessible Chromatin (ATAC-seq) emerged as an efficient method to chart open chromatin genome wide. The application of such technique to different cell types allowed unmasking tissue-specific regulatory elements and characterizing cis-regulatory networks. Herein we describe the implementation of the ATAC-seq method to human pancreatic islets, a tissue playing a central role in the control of glucose metabolism.

Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization.

HP1 is a structural component of heterochromatin. Mammalian HP1 isoforms HP1α, HP1ß, and HP1γ play different roles in genome stability, but their precise role in heterochromatin structure is unclear. Analysis of Hp1α-/-, Hp1ß-/-, and Hp1γ-/- MEFs show that HP1 proteins have both redundant and unique functions within pericentric heterochromatin (PCH) and also act globally throughout the genome. HP1α confines H4K20me3 and H3K27me3 to regions within PCH, while its absence results in a global hyper-compaction of chromatin associated with a specific pattern of mitotic defects. In contrast, HP1ß is functionally associated with Suv4-20h2 and H4K20me3, and its loss induces global chromatin decompaction and an abnormal enrichment of CTCF in PCH and other genomic regions. Our work provides insight into the roles of HP1 proteins in heterochromatin structure and genome stability.

An HP1 isoform-specific feedback mechanism regulates Suv39h1 activity under stress conditions.

The presence of H3K9me3 and heterochromatin protein 1 (HP1) are hallmarks of heterochromatin conserved in eukaryotes. The spreading and maintenance of H3K9me3 is effected by the functional interplay between the H3K9me3-specific histone methyltransferase Suv39h1 and HP1. This interplay is complex in mammals because the three HP1 isoforms, HP1α, ß, and γ, are thought to play a redundant role in Suv39h1-dependent deposition of H3K9me3 in pericentric heterochromatin (PCH). Here, we demonstrate that despite this redundancy, HP1α and, to a lesser extent, HP1γ have a closer functional link to Suv39h1, compared to HP1ß. HP1α and γ preferentially interact in vivo with Suv39h1, regulate its dynamics in heterochromatin, and increase Suv39h1 protein stability through an inhibition of MDM2-dependent Suv39h1-K87 polyubiquitination. The reverse is also observed, where Suv39h1 increases HP1α stability compared HP1ß and γ. The interplay between Suv39h1 and HP1 isoforms appears to be relevant under genotoxic stress. Specifically, loss of HP1α and γ isoforms inhibits the upregulation of Suv39h1 and H3K9me3 that is observed under stress conditions. Reciprocally, Suv39h1 deficiency abrogates stress-dependent upregulation of HP1α and γ, and enhances HP1ß levels. Our work defines a specific role for HP1 isoforms in regulating Suv39h1 function under stress via a feedback mechanism that likely regulates heterochromatin formation.

Residual urinary volume is a risk factor for primary nonfunction in kidney transplantation.

Primary nonfunction is a severe complication after kidney transplantation. Residual renal function could be a risk factor for this complication in the current era of kidney transplantation from extended criteria donors (ECD). This is a single-center case-control study. Between 2000 and 2012, 1112 patients received a kidney transplant from a deceased donor. We identified 56 cases of early graft loss (kidney that never recover renal function and/or graft thrombosis <48 h after kidney transplantation). As controls we used patients receiving the contralateral kidney. Donor age was 55 ± 17 years with 57% fulfilling ECD criteria. Among the 56 cases, 14 were due to vascular rejection and 42 to primary nonfunction. Risk factors for early graft loss due to vascular rejection were previous transplant, time on dialysis, and HLA sensitization. Risk factors for primary nonfunction were first transplant, short period on dialysis, mainly peritoneal dialysis, and a residual urinary volume ≥500 ml/24 h. Conditional logistic regression analysis showed that residual urinary volume (OR = 20.01) rather than the dialysis modality was a major risk factor for primary nonfunction. In conclusion, residual urinary volume seems to be a risk factor for primary nonfunction in the current era of kidney transplantation.

Influence of changing the distance of the 3-point line in basketball

Los objetivos del presente estudio fueron: 1) comprobar la influencia de la modificación de la distancia en la línea que delimita el triple (de 6.25 a 6.75 metros) sobre el porcentaje de acierto en los tiros de 3 puntos, durante los partidos de liga regular y playoff y 2) determinar si dicha modificación influye en la cantidad de tiros realizados por los diferentes puestos específicos en baloncesto (base, escolta, alero, ala pívot y pívot), así como en el porcentaje de acierto. Los datos se obtuvieron de la web oficial de la ACB (www.acb.com) e incluyen todos los partidos de la liga regular y del playoff jugados durante las temporadas 2009-2010 (n = 290 partidos y 7185 lanzamientos de 3 puntos) y 2010-2011 (n = 288 partidos y 6145 lanzamientos de 3 puntos); registrando, únicamente, a los jugadores que participaron ambas temporadas en dicha competición en el mismo equipo (n = 115). Los principales resultados indican que el número de tiros de 3 realizados en la temporada 2009/2010 es superior a los realizados en la temporada siguiente, 2010/2011, en la que se retrasa la línea que delimita el triple. Se puede apreciar, en la comparación de ambas temporadas, la existencia de diferencias estadísticamente significativas entre aleros por el número de lanzamientos intentados, entre todos los puestos por el número de lanzamientos convertidos y entre escoltas en los porcentajes de acierto del conjunto del equipo; diferencias que no se manifiestan en los partidos de playoff (AU)

The objectives of this study were 1) to test the influence of changing the distance in the 3-point line (from 6.25 to 6.75 meters.) on the percentage of success in the 3-point shots during the regular season and playoff games and 2) to determine if the changes affect the number of shots attempted by their different specific positions in basketball (guards, forwards and centers), and in the percentage of shots made. The data were obtained from the official website of the ACB (www.acb.com) and include all games in the regular season and playoff games during 2009-2010 (n = 290 games and 7185 3-point shots) and 2010-2011 (n = 288 and 6145 3-point shots). Only the players who played both seasons for the same team were taken into account (n = 115). The main results indicate that the number of 3-point shots in the season 2009/2010 is higher than the following one, 2010/2011, when the distance was lengthened. It can be seen in the comparison of both years, the existence of statistically significant differences between small-forwards by the number of shot attempts; also there were obtained a difference in all positions by the number of shots made and a difference in the percentages for shootingguards; these differences were are not observed in the playoffs (AU)

Performance factors in women's team handball: physical and physiological aspects--a review.

Team handball is an Olympic sport played professionally in many European countries. Nevertheless, a scientific knowledge regarding women's elite team handball demands is limited. Thus, the purpose of this article was to review a series of studies (n = 33) on physical characteristics, physiological attributes, physical attributes, throwing velocity, and on-court performances of women's team handball players. Such empirical and practical information is essential to design and implement successful short-term and long-term training programs for women's team handball players. Our review revealed that (a) players that have a higher skill level are taller and have a higher fat-free mass; (b) players who are more aerobically resistant are at an advantage in international level women team handball; (c) strength and power exercises should be emphasized in conditioning programs, because they are associated with both sprint performance and throwing velocity; (d) speed drills should also be implemented in conditioning programs but after a decrease in physical training volume; (e) a time-motion analysis is an effective method of quantifying the demands of team handball and provides a conceptual framework for the specific physical preparation of players. According to our results, there are only few studies on on-court performance and time-motion analysis for women's team handball players, especially concerning acceleration profiles. More studies are needed to examine the effectiveness of different training programs of women's team handball players' physiological and physical attributes.

Relationship between characteristics of water polo players and efficacy indices.

The aim of this study was to define and examine the relationships between the anthropometrical characteristics, maximum isometric grip strength, and competition throwing velocities and efficacy indices in high-level water polo player. Eleven elite trained male water polo players participated in this study. During preseason training, the following measures were taken: standard anthropometry (height, body mass, arm spam, skinfolds, body girths, and skeletal breadths) and grip strength. During official European Competitions (n = 7), efficacy indices (offensives: shot definition, resolution, precision, blocked and defensives: shot resolution when defending and shots stopped when defending), average and maximum throwing velocities from all the participants by zones and in some offensive tactical phases (even, counterattacks and power play) were also determined. Throwing velocities were different (p ≤ 0.05) between some of the offensive tactical phases (even = 17.9 ± 2.4 vs. power play = 16.7 ± 2.6 m·s(-1)). In addition, significant correlations were found between competitive throwing velocities and different offensive efficacy indices. We concluded that there were significant correlations between conditioning and performance variables with anthropometrical characteristics and offensive tactical indices (blocked shots received and shot precision). Coaches should pay attention to these indices for the development of performance throughout the season.

Anthropometric profile, vertical jump, and throwing velocity in elite female handball players by playing positions.

Women's handball is a sport, which has seen an accelerated development over the last decade. Although anthropometric and physical characteristics have been studied for male sports teams, in women's handball, studies are scarce. The aim of this study was twofold: first, to describe the anthropometric characteristics, throwing velocity, hand grip, and muscular power of the lower limbs in female handball players and second, to identify the possible differences in these parameters in terms of individual playing positions (center, back, wing, pivot, and goalkeeper). A total of 130 elite female Spanish handball players participated in the study (age 25.74 ± 4.84 years; playing experience 14.92 ± 4.88 years). Anthropometric assessment was performed for all the subjects following the International Society for the Advancement of Kinanthropometry protocols. Furthermore, all the subjects performed a vertical jump test (squat jump and countermovement jump). Hand grip and throwing velocity in several situations were also assessed. A 1-way analysis of variance and a Tukey post hoc test were used to study the differences among individual playing positions. Wings were less heavy, shorter, and showed a smaller arm span than did goalkeepers, backs and pivots (p ≤ 0.001). Additionally, pivots were heavier than centers. Backs and pivots exhibited higher muscular mass than did wings. Total players' somatotype was mesomorphy endomorphy (3.89-4.28-2.29). Centers showed higher throwing velocity levels than did wings in 9-m throws from just behind the line, with a goalkeeper. Backs exhibited higher hand-grip values than did wings. Statistical differences have been established between wings and other specific playing positions, especially with pivot and backs. Coaches can use this information to select players for the different specific positions.

Throwing velocities, anthropometric characteristics, and efficacy indices of women's European water polo subchampions.

Water polo is a team sport characterized by a high-intensity, intermittent activity, where technical and tactical aspect are of a great importance. For that reason, the main aim of this study was to define the anthropometrical characteristics, maximum isometric grip strength, training and competition throwing velocities, and the efficacy indices in female high-level water polo players. A second purpose was to examine the differences between the throwing velocities in training vs. European championships in the water polo female national team. Ten elite trained female water polo players participated in this study. Before the competitive phase of their season, the following measures were taken: standard anthropometry, static and dynamic training throwing velocities, and hand-grip dynamometry. In the competitive phase, efficacy indices, average and maximum throwing velocities from all the participants were also determined. Significant differences (p ≤ 0.05) were found between different training situations and different competitive throwing velocities. We concluded that elite female water polo players modify their throwing velocity depending if the throw is performed during training or competitive situation.