[Production and partial purification of the main hemolysin (pneumolysin) of Streptococcus pneumoniae]. / Producción y purificación parcial de la hemolisina principal (Neumolisina) de Streptococcus pneumoniae.

The capacity of 36 Cuban strains of Streptococcus pneumoniae to produce pneumolysin was studied, and 94.4% of them were determined to be producers of that enzyme. One of the best producers was cultured at a great scale and the pneumolysin found in the supernatan was partially purified through an ion-exchange chromatography in mono-Q column. This method made it possible to recover the enzyme whose purity level increased by 4.39 with 100% output.