Vitamin C inhibits random migration of malignant pleural effusion mononuclear cells.

Although many epidemiological studies indicate protective effect of vitamin C against a variety of human malignancies its mechanism(s) of action is questionable. The presented results show that the part of its effect may be accomplished by mononuclear cells, as necessary participants in body defence. Namely, in a long-term in vitro assay we tested vitamin C influence on random migration ability of malignant pleural effusion mononuclears (PEM) obtained from breast cancer patients. Vitamin C in a dose- (50-500 micrograms) and time-dependent (4-44 h) manner inhibited PEM motility, suggesting that immobilization of cells in situ may contribute to its beneficial effect in human cancers.

Negative correlation between maorophage proooagulant and migraion ability in the course of inflammation.

The importance of macrophage procoagulant activity (PCA) to cell migration is presumed. In this study we assayed the relationship between the two functions in guinea-pig peritoneal resident macrophages and cells elicited by a sterile inflammation induction, which lasted up to 6 days. The findings pointed to an in vivo induction of PCA in macrophages, which declined with time during inflammation. A clear negative correlation between PCA and random migration ability was demonstrated. Our results suggest that the local induction of coagulation by macrophages may immobilize the cells at the site of inflammation.

Impaired random migration and special reactivity to methylprednisolone in young guinea pig macrophages.

Macrophage (M psi) migration ability and its susceptibility to methylprednisolone (MP) action, both in vivo and in vitro, were compared in young and adult guinea pigs. The random migration of oil-induced peritoneal M psi was tested by the capillary tube method. The observation of M psi locomotion from 4 to 44 h of incubation showed that cells obtained from young animals had a smaller migration ability than those of adults. Besides that, differences in reactivity to MP were observed between the compared groups. The application of MP in vivo led to the stimulation of young M psi migration in vitro, but inhibited adult M psi migration. The exposure of adult M psi to MP in vitro did not affect their motility, but migration of the young M psi, as previously, was stimulated. Thus, the presented findings report about the immaturity of young M psi random migration and its special reactivity to MP.

Modulation of guinea pig peritoneal macrophage migration in vitro: effects of protease inhibitors.

With the purpose of examining the significance of macrophage neutral proteases for the random migration of guinea pig peritoneal macrophages, we tested the influence of the polyvalent protease inhibitor, Trasylol (100-2000 KIU/ml), and the serine protease inhibitor, phenylmethyl sulphonyl fluoride (PMSF; 10(-4)-10(-3) M), on this function. Using the capillary method, the migration of resident and oil-induced cells was examined under different culture conditions: absence of serum; presence of 10% of either intact (IHS) or acid-treated horse serum (AHS). Protease inhibitors only reduced the locomotion of inflammatory macrophages. Trasylol caused a dose-dependent reversible macrophage migration inhibition the degree of which depended upon the conditions in culture (AHS greater than no HS greater than IHS). The irreversible effects of PMSF were better in the presence of serum. Although the migration of control macrophages occurred at the three different quantitative levels (AHS greater than IHS greater than no HS), there were no differences in the migration kinetics under the action of these antiproteases compared to the corresponding control, which, together with the preserved cell viability indicates that the drugs did not act deleteriously on macrophages. Our results suggest that macrophage neutral proteases do not only play an important role in delayed hypersensitivity, as previously demonstrated, but also in the random migration of inflammatory macrophages. Furthermore, it is shown that the clinical application of Trasylol may have an influence on this vitally important macrophage function.

2-mercaptoethanol in vitro and macrophage migration in normal and sensitized guinea pigs.

While the influence of 2-mercaptoethanol (2-ME) on the functions of lymphocytes has rather extensively been investigated, there are almost no data on the action of this thiol compound on macrophages. We examined the effect of 2-ME in vitro (10(-6)-10(-4) M) on migration from capillary tubes of oil elicited peritoneal macrophages of non-immune and immune guinea pigs, sensitized with ovalbumin in complete Freund's adjuvant. This thiol did not affect the random migration of macrophages both in normal and in sensitized animals. Contrary to this, it significantly enhanced the frequency of occurrence and the magnitude of migration inhibition caused by the action of the migration inhibitory factor (MIF) in the examination of delayed hypersensibility by a direct MIF assay. The possible mechanism and the site of action of 2-ME are discussed.

Effect of prednisolone in vitro on the migration ability of peritoneal macrophages in rats.

Prednisolone in vitro decreases the random migration of peritoneal macrophages in non-sensitized rats. In sensitized animals cells are sensitive or resistant to prednisolone's inhibitory action, depending on the presence of antigen in the culture.