Piglets vocally express the anticipation of pseudo-social contexts in their grunts.

Emotions not only arise in reaction to an event but also while anticipating it, making this context a means of accessing the emotional value of events. Before now, anticipatory studies have rarely considered whether vocalisations carry information about emotional states. We studied both the grunts of piglets and their spatial behaviour as they anticipated two (pseudo)social events known to elicit positive emotions of different intensity: arrival of familiar conspecifics and arrival of a familiar human. Piglets spatially anticipated both pseudo-social contexts, and the spectro temporal features of grunts differed according to the emotional context. Piglets produced low-frequency grunts at a higher rate when anticipating conspecifics compared to anticipating a human. Spectral noise increased when piglets expected conspecifics, whereas the duration and frequency range increased when expecting a human. When the arrival of conspecifics was delayed, the grunt duration increased, whereas when the arrival of the human was delayed, the spectral parameters were comparable to those during isolation. This shows that vocal expressions in piglets during anticipation are specific to the expected reward. Vocal expressions-both their temporal and spectral features- are thus a good way to explore the emotional state of piglets during the anticipation of challenging events.

Stress-induced flexibility and individuality in female and male zebra finch distance calls.

Vocal recognition is central to the coordination and organization of behavior in pair-bonding species such as zebra finches. Zebra finches' vocalizations are individualized and support acoustic discrimination processes. Physiological states - such as the ones involved in emotional stress - can modify vocal production and consequently the structure of vocalizations. These modifications might signal the state of the caller but also impair individual recognition processes. This may represent a signaling trade-off, especially in contexts where both pieces of information can be critically important, for example when mates use calls to reunite after social isolation. Here we study the impact of a stress on the individual vocal signature in both female and male zebra finch distance calls. We built a manually curated database of distance calls of several individuals (both females and males) recorded in control and stress conditions. The stress was induced either by social isolation of the bird or using exogenous corticosterone. We developed a machine learning approach to assess the impact of stress on the individual characterization of calls. We show that while calls' spectral structure is significantly modified by stress, it still allows for the correct classification of calls to the caller. Moreover, we also show that the stress-induced modification of calls' structure is not a 'general feature signal' that can be detected as a 'stress' signal regardless of identity. Thus, female and male zebra finch calls' structure show stress-induced flexibility that stays within the range of individual vocal signatures.