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1.
Biodivers Data J ; 11: e72764, 2023.
Article in English | MEDLINE | ID: mdl-36761087

ABSTRACT

Background: Rhiniinae (Diptera, Calliphoridae) is a taxon of nearly 400 known species, many of them termitophilous. Approximatelly 160 valid species in 16 genera are Afrotropical, with over 60 of them occurring in South Africa. The taxonomy of this group is outdated, as most studies of the South African taxa were conducted 40 to 70 years ago (mostly by Salvador Peris and Fritz Zumpt). Published information on their biology and ecology is also scarce. New information: An annotated checklist of 73 species of Rhiniinae for South Africa was developed, based on the holdings of sixteen entomological collections in Africa, Europe and North America. Over 3,700 specimens were examined, revealing nine new species records for South Africa (Cosminaundulata Malloch, 1926, Isomyiacuthbertsoni (Curran, 1938), Rhyncomyabotswana Zumpt, 1974, R.tristis Séguy, 1933, Stomorhinaapta Curran, 1931, S.malobana (Lehrer, 2007), Thoraciteskirkspriggsi Kurahashi, 2001, Th.sarcophagoides Kurahashi, 2001 and Trichoberialanata (Villeneuve, 1920)). We propose one new combination Eurhyncomyiametzi (Zumpt, 1981) comb. nov. (= Rhyncomyametzi Zumpt, 1981)). Additionally, evidence is presented to remove Rhyncomyaviduella Villeneuve, 1927 stat. rev. from synonymy with Rhyncomyacassotis (Walker, 1849). Relevant novel biological and seasonality information, historical occurrence maps and high-definition photographs for each species are compiled.

2.
Zookeys ; 1033: 127-157, 2021.
Article in English | MEDLINE | ID: mdl-33958922

ABSTRACT

The taxonomy and diversity of Fainia Zumpt, 1958, an exclusive Afrotropical genus, had not been reviewed recently. The genus included six nominal species, but the status of several of them was debated. Identification of most Fainia species depends on characters of the male terminalia; females are poorly known and, in several cases, are not adequately diagnosed. We conducted a taxonomic revision of the genus and generated identification tools. Based on the study of type material and specimens available in entomological collections in Africa and Europe, we recognise here three of the six species as valid (F. albitarsis (Macquart, 1846), F. elongata (Bezzi, 1908) and F. inexpectata Zumpt, 1973). We also provide an identification key to both sexes, redescriptions of the species, updated distribution records and high resolution photographs of males' and females' habitus and male terminalia. The description of Fainia kagerana Lehrer, 2007a nom. nud. is an invalid nomenclatural act in terms of ICZN Article 13.1.1. Based on examinations of their holotypes, F. sambura Lehrer, 2008 syn. nov. is proposed as a junior synonym of F. albitarsis; F. kirinyaga Lehrer, 2007b syn. nov. is proposed as a junior synonym of F. inexpectata; and Fainia giriama Lehrer, 2007b is moved from the genus Fainia to the genus Rhinia Robineau-Desvoidy, as Rhinia giriama (Lehrer, 2007b) comb. nov.. We propose two apomorphies that support the status of the subfamily Rhiniinae.

3.
Zootaxa ; 4885(4): zootaxa.4885.4.7, 2020 Nov 27.
Article in English | MEDLINE | ID: mdl-33311262

ABSTRACT

Ingcainyenzane irhiniensis n. gen., n. sp. and Ingcainyenzane nolukhanyoensis n. gen., n. sp. are described from Eastern Cape and Ingcainyenzane umgeniensis n. gen., n. sp. is described from KwaZulu-Natal, South Africa. Notes on its biology of the species and a key to species of the genus are also provided.


Subject(s)
Hemiptera , Animals , South Africa
4.
Zookeys ; 936: 1-24, 2020.
Article in English | MEDLINE | ID: mdl-32547291

ABSTRACT

This study investigates genetic diversity in three species of Ephemeroptera, one eurytopic and therefore widespread (Afroptilum sudafricanum) and two stenotopic and thus endemic (Demoreptus natalensis and Demoreptus capensis) species, all of which co-occur in the southern Great Escarpment, South Africa. Mitochondrial DNA was analysed to compare the genetic diversity between the habitat generalist and the two habitat specialists. Afroptilum sudafricanum showed no indication of population genetic structure due to geographic location, while both Demoreptus species revealed clear genetic differentiation between geographic localities and catchments, evident from phylogenetic analyses and high FST values from AMOVA. In addition, the phylogenetic analyses indicate some deeper haplotype divergences within A. sudafricanum and Demoreptus that merit taxonomic attention. These results give important insight into evolutionary processes occurring through habitat specialisation and population isolation. Further research and sampling across a wider geographic setting that includes both major mountain blocks of the Escarpment and lowland non-Escarpment sites will allow for refined understanding of biodiversity and associated habitat preferences, and illuminate comparative inferences into gene flow and cryptic speciation.

5.
Zootaxa ; 4731(4): zootaxa.4731.4.6, 2020 Feb 10.
Article in English | MEDLINE | ID: mdl-32230285

ABSTRACT

Afromelampsalta n. gen. is described for African species currently assigned to the genera Melampsalta Kolenati, 1857. Cicadetta Kolenati, 1857 and Pauropsalta Goding Froggatt, 1904, and the new species Afromelampsalta luteofasciata n. gen., n. sp. is described. Afromelampsalta aethiopica (Distant, 1905) n. comb., A. cadisia (Walker, 1850) n. comb. and A. leucoptera (Germar, 1830) n. comb. are reassigned from Melampsalta to Afromelampsalta n. gen., A. limitata (Walker, 1852) n. comb. is transferred from Cicadetta Kolenati, 1857 and A. mimica (Distant ,1907) n. comb. is transferred from Pauropsalta Goding Froggatt, 1904 to Afromelampsalta n. gen. Notes on the biology of the new species, a description of the exuvia of A. mimica n. comb., and a key to the species of African Cicadettini are provided.


Subject(s)
Hemiptera , Animals , Biology
6.
Int J Legal Med ; 134(2): 793-810, 2020 Mar.
Article in English | MEDLINE | ID: mdl-31209558

ABSTRACT

Most studies of decomposition in forensic entomology and taphonomy have used non-human cadavers. Following the recommendation of using domestic pig cadavers as analogues for humans in forensic entomology in the 1980s, pigs became the most frequently used model cadavers in forensic sciences. They have shaped our understanding of how large vertebrate cadavers decompose in, for example, various environments, seasons and after various ante- or postmortem cadaver modifications. They have also been used to demonstrate the feasibility of several new or well-established forensic techniques. The advent of outdoor human taphonomy facilities enabled experimental comparisons of decomposition between pig and human cadavers. Recent comparisons challenged the pig-as-analogue claim in entomology and taphonomy research. In this review, we discuss in a broad methodological context the advantages and disadvantages of pig and human cadavers for forensic research and rebut the critique of pigs as analogues for humans. We conclude that experiments using human cadaver analogues (i.e. pig carcasses) are easier to replicate and more practical for controlling confounding factors than studies based solely on humans and, therefore, are likely to remain our primary epistemic source of forensic knowledge for the immediate future. We supplement these considerations with new guidelines for model cadaver choice in forensic science research.


Subject(s)
Forensic Entomology/methods , Forensic Sciences/trends , Models, Animal , Research Design/trends , Swine , Animals , Cadaver , History, 19th Century , History, 20th Century , History, 21st Century , Humans
7.
Mol Phylogenet Evol ; 131: 193-210, 2019 02.
Article in English | MEDLINE | ID: mdl-30278252

ABSTRACT

The genus Chondrocyclus Ancey, 1898 contains the majority of southern African members of the Cyclophoridae, a large family of operculate land snails. We present the first molecular phylogeny of the genus based on two mitochondrial genes (16S and CO1) and complement this with an appraisal of morphological characters relating to the shell and soft parts. Worn shells on which some descriptions and records of different species were based appear to be indistinguishable morphologically, creating taxonomic confusion. We show that Chondrocyclus s.l. underwent two major radiations, one Afromontane and the other largely coastal. Accordingly, we recommend a revision recognising two genera. Chondrocyclus s.s. contains four monophyletic lineages, each characterized by a combination of morphological features. The Afromontane group is shown to be a species complex; relationships within this complex could not be resolved due to insufficient DNA sequence data. The molecular data confirms the monophyly of seven currently recognised species and provides evidence for at least twelve undescribed species; the morphological data are broadly consistent with this finding. The morphological data suggest that the two species from countries to the north of South Africa should be removed from the genus, and that Chondrocyclus sensu lato is endemic to South Africa. The historical biogeography of this group of microhabitat specialists with poor dispersal abilities contributes an additional, phylogenetically independent taxon to our understanding of the processes generating biodiversity in southern Africa, a natural laboratory for palaeobiogeography. All taxa are narrow-range endemics, underlining the importance of conserving South Africa's threatened forest habitats.


Subject(s)
Ecosystem , Phylogeny , Snails/classification , Snails/genetics , Animals , Base Sequence , Bayes Theorem , Databases, Genetic , Geography , Snails/anatomy & histology , South Africa , Species Specificity
8.
Forensic Sci Res ; 3(1): 2-15, 2018.
Article in English | MEDLINE | ID: mdl-30483647

ABSTRACT

Chrysomya megacephala (Fabricius, 1794) occurs on every continent and is closely associated with carrion and decaying material in human environments. Its abilities to find dead bodies and carry pathogens give it a prominence in human affairs that may involve prosecution or litigation, and therefore forensic entomologists. The identification, geographical distribution and biology of the species are reviewed to provide a background for approaches that four branches of forensic entomology (urban, stored-product, medico-criminal and environmental) might take to investigations involving this fly.

9.
Zootaxa ; 4424(1): 1-64, 2018 May 28.
Article in English | MEDLINE | ID: mdl-30313477

ABSTRACT

A molecular phylogeny and a review of family-group classification are presented for 137 species (ca. 125 genera) of the insect family Cicadidae, the true cicadas, plus two species of hairy cicadas (Tettigarctidae) and two outgroup species from Cercopidae. Five genes, two of them mitochondrial, comprise the 4992 base-pair molecular dataset. Maximum-likelihood and Bayesian phylogenetic results are shown, including analyses to address potential base composition bias. Tettigarcta is confirmed as the sister-clade of the Cicadidae and support is found for three subfamilies identified in an earlier morphological cladistic analysis. A set of paraphyletic deep-level clades formed by African genera are together named as Tettigomyiinae n. stat. Taxonomic reassignments of genera and tribes are made where morphological examination confirms incorrect placements suggested by the molecular tree, and 11 new tribes are defined (Arenopsaltriini n. tribe, Durangonini n. tribe, Katoini n. tribe, Lacetasini n. tribe, Macrotristriini n. tribe, Malagasiini n. tribe, Nelcyndanini n. tribe, Pagiphorini n. tribe, Pictilini n. tribe, Psaltodini n. tribe, and Selymbriini n. tribe). Tribe Tacuini n. syn. is synonymized with Cryptotympanini, and Tryellina n. syn. is synonymized with an expanded Tribe Lamotialnini. Tribe Hyantiini n. syn. is synonymized with Fidicinini. Tribe Sinosenini is transferred to Cicadinae from Cicadettinae, Cicadatrini is moved to Cicadettinae from Cicadinae, and Ydiellini and Tettigomyiini are transferred to Tettigomyiinae n. stat from Cicadettinae. While the subfamily Cicadinae, historically defined by the presence of timbal covers, is weakly supported in the molecular tree, high taxonomic rank is not supported for several earlier clades based on unique morphology associated with sound production.


Subject(s)
Hemiptera , Phylogeny , Animals , Bayes Theorem , Insecta
10.
Int J Legal Med ; 132(3): 831-842, 2018 May.
Article in English | MEDLINE | ID: mdl-28849264

ABSTRACT

Necrophagous blowflies can provide an excellent source of evidence for forensic entomologists and are also relevant to problems in public health, medicine, and animal health. However, access to useful information about these blowflies is constrained by the need to correctly identify the flies, and the poor availability of reliable, accessible identification tools is a serious obstacle to the development of forensic entomology in the majority of African countries. In response to this need, a high-quality key to the adults of all species of forensically relevant blowflies of Africa has been prepared, drawing on high-quality entomological materials and modern focus-stacking photomicroscopy. This new key can be easily applied by investigators inexperienced in the taxonomy of blowflies and is made available through a highly accessible online platform. Problematic diagnostic characters used in previous keys are discussed.


Subject(s)
Diptera/anatomy & histology , Forensic Sciences , Africa , Animals , Diptera/physiology , Entomology , Feeding Behavior , Humans , Postmortem Changes
11.
Int J Legal Med ; 131(5): 1399-1412, 2017 Sep.
Article in English | MEDLINE | ID: mdl-28567525

ABSTRACT

Forensic entomotoxicology is the use of insects as evidence of whether a toxicant is present in an environment such as a corpse, river or landscape. The earliest overtly forensic study was published in 1977, and since then, at least 63 papers have been published, most of them focused on the detection of toxicants in insects or on effects of toxicants on diverse insect indicator taxa. A comprehensive review of the published literature revealed various inconsistencies between studies that could be addressed by introducing standard protocols for such studies. These protocols could include selecting widespread and common model organisms (such as Lucilia sericata, Calliphora vicina, Chrysomya megacephala and Dermestes maculatus) and model toxicants (e.g. morphine and amitriptyline) to build up comparative databases; developing a standard matrix for use as a feeding substrate; setting guidelines for statistically adequate sample sizes; and deploying more sophisticated analytical methods from the general field of toxicology. Future studies should then be aimed at refining standardised protocols to improve experimental results, and make these results more comparable between studies.


Subject(s)
Entomology , Feeding Behavior , Forensic Toxicology , Postmortem Changes , Research Design/standards , Animals , Elements , Humans , Illicit Drugs , Insecta , Pharmaceutical Preparations , Pharmacokinetics
12.
Forensic Sci Med Pathol ; 13(2): 123-134, 2017 Jun.
Article in English | MEDLINE | ID: mdl-28409386

ABSTRACT

Published research has offered contradictory evidence of the occurrence of nocturnal oviposition by carrion-breeding blowflies, a behavior that can affect the interpretation of forensic estimates of a minimum post mortem interval (minPMI) by up to 12 hours, depending on latitude and season. The majority of published studies are from the northern hemisphere. Field experiments were conducted in South Africa and Australia that extend observations to species of the southern hemisphere. Various vertebrate carrion was exposed at night in summer under different lunar phases and/or artificial lighting, and in woodland and pasture areas. Three laboratory experiments were also conducted. No nocturnal oviposition occurred outdoors in Berry, Australia, but Lucilia cuprina, Lucilia sericata and Chrysomya megacephala laid eggs outdoors at night in Grahamstown and Durban, South Africa. In laboratory experiments L. sericata, L. cuprina, Chrysomya chloropyga and Chrysomya putoria laid eggs and Calliphora augur deposited larvae under nocturnal conditions. Chrysomya albiceps and C. chloropyga laid eggs in darkness with increasing likelihood as ambient temperature increased. This study shows that nocturnal ovi/larviposition by carrion-breeding blowflies is possible in both South Africa and Australia. The forensic issue is therefore not whether nocturnal oviposition occurs, but rather whether the conditions of a particular case are more or less conducive to it. Circadian rhythms and physiological thresholds (particularly temperature and humidity) appear to act individually and in conjunction to stimulate or inhibit nocturnal laying. The significance of carcass size, freezing and handling of carcasses and comprehensive quantification for experimental design is discussed, and recommendations are made for future laboratory and case scene experiments.


Subject(s)
Circadian Rhythm/physiology , Diptera/physiology , Oviposition/physiology , Animals , Australia , Feeding Behavior , Humidity , Postmortem Changes , South Africa , Temperature
13.
Biodivers Data J ; (4): e10059, 2016.
Article in English | MEDLINE | ID: mdl-27932920

ABSTRACT

BACKGROUND: Traditional agro-pastoral practices are in decline over much of the Alps (MacDonald et al. 2000), leading to shrub and tree encroachment, and this represents one of the main threats for the conservation of alpine biodiversity, as many plant and animal species are dependent on the presence of semi-natural open habitats. However, quantifying this environmental change and assessing its impact on biodiversity may be difficult, especially in the context of sparse historical survey data. The accessibility of contemporary data about local biodiversity surveys in general, and indicator taxa in particular, is an essential consideration for planning future evaluations of conservation status in the Alps and for conservation plans that use ecological indicators to monitor temporal changes in biodiversity. Dung beetles are important ecosystem service providers (Nichols et al. 2008) that have been assessed as a good ecological indicator taxon in several studies (reviewed by Nichols and Gardner 2011), and although the Alps is perhaps one of the best-studied regions in respect of dung beetles, there are still only eight readily-accessible publications. We have augmented and comprehensively reviewed the data from these publications. NEW INFORMATION: We first provide data about changes on a temporal scale of seasons in a dung beetle community in the western Italian Alps, an issue that has to be addressed in the local assemblages because it would affect regional biomonitoring and conservation research. This survey of 12 099 individuals belonging to 22 species illustrates a distinct seasonal pattern at a single site. Second, we collate the results of 13 published surveys of the presence of 46 species of dung beetles in 11 valleys in the western Italian Alps in the period from 2005 to 2012, a period of accelerated change in land use that started around 1945 (MacDonald et al. 2000). Because ten of the surveys used baited pitfall traps and four more used manual collection of specimens, the abundance data were not strictly comparable and they were therefore transformed to binary data (presence-or-absence records) with measures of sampling effort. The results illustrate both spatial variation and temporal variation at the scale of years.Because of the importance of dung beetles in agro-pastoral ecosystems and the high sensitivity of montane ecosystems to climate change, these spatially and temporally explicit data sets provide important baseline information about western Italian Alpine dung beetles for investigations of the effects of land use change under ongoing climate change scenarios.

14.
J Insect Physiol ; 95: 98-104, 2016 12.
Article in English | MEDLINE | ID: mdl-27646733

ABSTRACT

Blowfly larvae aggregate on exposed carcasses and corpses and pass through three instars before wandering from the carcass and pupating. The developmental landmarks in this process can be used by forensic entomologists to estimate the time since the insects colonised the carcass, which sets a minimum post mortem interval. Large aggregations of feeding larvae generate a microclimate with temperatures up to 15°C above ambient conditions, which may accelerate larval development and affect forensic estimates of post-mortem intervals. This study investigated the effects of heat accumulated by maggot masses of Lucilia cuprina at aggregations of 20, 50 and 100 larvae, each at incubation temperatures of 18°C, 24°C and 30°C, using body length and life stage as developmental indicators. Aggregation temperatures reached up to 18.7°C above ambient temperature, with significant effects of both size and temperature of the aggregation on the development time of its larvae. Survivorship was highest for all life stages at 24°C, which is near the developmental optimum of L. cuprina. The results of this study provide a broadly applicable method of quantifying heat accumulation by aggregations of a wide range of species of forensic importance, and the results obtained from such studies will demonstrate that ambient temperature cannot be considered the only source of heat that blowfly larvae experience when they develop on a carcass. Neglect of temperatures within larval aggregations will result in an overestimation of post-mortem intervals and thus have far-reaching medicolegal consequences.


Subject(s)
Diptera/growth & development , Forensic Sciences/methods , Animals , Hot Temperature , Larva/growth & development , Longevity , Population Density
15.
Zootaxa ; 4092(2): 195-206, 2016 Mar 15.
Article in English | MEDLINE | ID: mdl-27394449

ABSTRACT

Two species, Xosopsaltria brachyptera n. sp. and Xosopsaltria vitripennis n. sp., are described as new. Notes on their biology and a key to the South African pygmy bladder cicadas are also provided.


Subject(s)
Hemiptera/anatomy & histology , Hemiptera/classification , Animal Distribution , Animals , Female , Hemiptera/physiology , Male , South Africa , Species Specificity
16.
Zookeys ; (568): 59-86, 2016.
Article in English | MEDLINE | ID: mdl-27103874

ABSTRACT

The subfamily Luciliinae is diverse and geographically widespread. Its four currently recognised genera (Dyscritomyia Grimshaw, 1901, Hemipyrellia Townsend, 1918, Hypopygiopsis Townsend 1916 and Lucilia Robineau-Desvoidy, 1830) contain species that range from saprophages to obligate parasites, but their pattern of phylogenetic diversification is unclear. The 28S rRNA, COI and Period genes of 14 species of Lucilia and Hemipyrellia were partially sequenced and analysed together with sequences of 11 further species from public databases. The molecular data confirmed molecular paraphyly in three species-pairs in Lucilia that hamper barcode identifications of those six species. Lucilia sericata and Lucilia cuprina were confirmed as mutual sister species. The placements of Dyscritomyia and Hypopygiopsis were ambiguous, since both made Lucilia paraphyletic in some analyses. Recognising Hemipyrellia as a genus consistently left Lucilia s.l. paraphyletic, and the occasionally-recognised (sub)genus Phaenicia was consistently paraphyletic, so these taxa should be synonymised with Lucilia to maintain monophyly. Analysis of a matrix of 14 morphological characters scored for adults of all genera and for most of the species included in the molecular analysis confirmed several of these findings. The different degrees of parasitism were phylogenetically clustered within this genus but did not form a graded series of evolutionary stages, and there was no particular relationship between feeding habits and biogeography. Because of the ubiquity of hybridization, introgression and incomplete lineage sorting in blow flies, we recommend that using a combination of mitochondrial and nuclear markers should be a procedural standard for medico-criminal forensic identifications of insects.

17.
Zookeys ; (641): 121-150, 2016.
Article in English | MEDLINE | ID: mdl-28138294

ABSTRACT

The river sardine, Mesobola brevianalis (Boulenger, 1908), is the type species of Mesobola Howes, 1984. Standard phylogenetic analyses of partial sequences of the cytochrome oxidase I gene of individuals from populations across southern Africa that are currently identified as Mesobola brevianalis showed that these populations represent four genetically distinct allopatric lineages. Furthermore, Engraulicypris sardella (Günther, 1868), the type species of Engraulicypris Günther, 1894, was convincingly nested amongst these clades. These findings support synonymisation of Engraulicypris and Mesobolasyn. n.; restoration of Engraulicypris gariepinus (Barnard, 1943), stat. rev. for the lower Orange River population; description of two new species, Engraulicypris ngalalasp. n. and Engraulicypris howesisp. n. from the Rovuma and Kunene river systems, respectively; affirmation of the synonymy of Engraulicypris brevianalis (Boulenger, 1908), comb. n. sensu stricto and Engraulicypris whitei van der Horst, 1934; and restoration of Engraulicypris bredoi Poll, 1945, stat. rev. and Engraulicypris spinifer Bailey & Matthes, 1971, stat. rev. from Mesobola. Discriminant function analysis of a truss network of five traditional morphometric measurements and 21 morphometric measurements that characterised the shape of the fishes was used to seek morphological markers for the genetically distinct populations. Only Engraulicypris gariepinus was morphometrically distinctive, but live colouration differed between the lineages. Detailed taxonomic descriptions and an identification key for the species are provided.

18.
Biodivers Data J ; (3): e5792, 2015.
Article in English | MEDLINE | ID: mdl-26379465

ABSTRACT

BACKGROUND: Sound collections for singing insects provide important repositories that underpin existing research (e.g. Price et al. 2007 at http://bio.acousti.ca/node/11801; Price et al. 2010) and make bioacoustic collections available for future work, including insect communication (Ordish 1992), systematics (e.g. David et al. 2003), and automated identification (Bennett et al. 2015). The BioAcoustica platform (Baker et al. 2015) is both a repository and analysis platform for bioacoustic collections: allowing collections to be available in perpetuity, and also facilitating complex analyses using the BioVeL cloud infrastructure (Vicario et al. 2011). The Global Cicada Sound Collection is a project to make recordings of the world's cicadas (Hemiptera: Cicadidae) available using open licences to maximise their potential for study and reuse. This first component of the Global Cicada Sound Collection comprises recordings made between 2006 and 2008 of Cicadidae in South Africa and Malawi. NEW INFORMATION: This collection of sounds includes 219 recordings of 133 voucher specimens, comprising 42 taxa (25 identified to species, all identified to genus) from South Africa and Malawi. The recordings have been used to underpin work on the species limits of cicadas in southern Africa, including Price et al. (2007) and Price et al. (2010). The specimens are deposited in the Albany Museum, Grahamstown, South Africa (AMGS). The harvesting of acoustic data as occurrence records by GBIF has been implemented by the Scratchpads Team at the Natural History Museum, London. This link increases the value of individual recordings and the BioAcoustica platform within the global infrastructure of biodiversity informatics by making specimen/occurence records from BioAcoustica available to a wider audience, and allowing their integration with other occurence datasets that also contribute to GBIF.

19.
Int J Legal Med ; 129(5): 1155-62, 2015 Sep.
Article in English | MEDLINE | ID: mdl-25634604

ABSTRACT

The blowfly Lucilia cuprina is a primary colonizer of decaying vertebrate carrion, and its development provides a temperature-dependent clock that may be used to estimate the post-mortem interval of corpses and carcasses in medicolegal forensic investigations. This study uses the development of L. cuprina raised on a substrate of chicken liver at six constant temperatures from 18 to 33 °C to calibrate a thermal accumulation model of development for forensic applications. Development was optimal near 24 °C; above this temperature, survival of post-feeding life stages was increasingly compromised, while below it, development was increasingly retarded. The lower developmental threshold (~12 °C) and thermal summation constants of L. cuprina are distinct from those reported for Lucilia sericata, verifying that it is essential to identify African Lucilia specimens accurately when using them to estimate post-mortem intervals.


Subject(s)
Diptera/growth & development , Temperature , Animals , Entomology , Feeding Behavior , Forensic Medicine , Incubators , Larva/growth & development , Oviposition , Postmortem Changes , Pupa/growth & development
20.
Zookeys ; (420): 69-85, 2014.
Article in English | MEDLINE | ID: mdl-25061373

ABSTRACT

Hybrids of Lucilia sericata and Lucilia cuprina have been shown to exist in previous studies using molecular methods, but no study has shown explicitly that these hybrids can be identified morphologically. Published morphological characters used to identify L. sericata and L. cuprina were reviewed, and then scored and tested using specimens of both species and known hybrids. Ordination by multi-dimensional scaling indicated that the species were separable, and that hybrids resembled L. cuprina, whatever their origin. Discriminant function analysis of the characters successfully separated the specimens into three unambiguous groups - L. sericata, L. cuprina and hybrids. The hybrids were morphologically similar irrespective of whether they were from an ancient introgressed lineage or more modern. This is the first evidence that hybrids of these two species can be identified from their morphology. The usefulness of the morphological characters is also discussed and photographs of several characters are included to facilitate their assessment.

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