Deciphering Bisphenol A degradation by Coelastrella sp. M60: unravelling metabolic insights through metabolomics analysis.

Microalgae, owing to their efficacy and eco-friendliness, have emerged as a promising solution for mitigating the toxicity of Bisphenol A (BPA), a hazardous environmental pollutant. This current study was focused on the degradation of BPA by Coelastrella sp. M60 at various concentrations (10-50 mg/L). Further, the metabolic profiling of Coelastrella sp. M60 was performed using GC-MS analysis, and the results were revealed that BPA exposure modulated the metabolites profile with the presence of intermediates of BPA. In addition, highest lipid (43%) and pigment content (40%) at 20 and 10 mg/L of BPA respectively exposed to Coelastrella sp. M60 was achieved and enhanced fatty acid methyl esters recovery was facilitated by Cuprous oxide nanoparticles synthesised using Spatoglossum asperum. Thus, this study persuades thepotential of Coelastrella sp. M60 for BPA degradation and suggesting new avenues to remove the emerging contaminants in polluted water bodies and targeted metabolite expression in microalgae.

Enhancement of lipid accumulation in microalga Desmodesmus sp. VV2: Response Surface Methodology and Artificial Neural Network modeling for biodiesel production.

Microalgae are the most attractive renewable energy sources for the production of biofuels because of their luxurious growth and lipid accumulation ability in diverse nutritional conditions. In the present study, Desmodesmus sp. VV2, an indigenous microalga, was evaluated for its biodiesel potential using Response Surface Methodology (RSM) to improve the lipid accumulation with the combination of nutrients stress NaNO3 starvation, CaCl2 depletion, and supplementation of magnesium oxide nanoparticles (MgO). Among different stress conditions, 57.6% lipid content was achieved from RSM optimized media. Owing to this, RSM results were also validated by the Artificial Neural Network (ANN) with 11 training algorithms and it is found that RSM was more significant. In addition, the saturated fatty acid (SFA) content was noticeably increased in RSM optimized media (95.8%) while compared with control. Further, the highest total FAME content 97.21% was also achieved in cells grown in RSM optimized media. Biodiesel quality parameters were analyzed and found that they are in accordance with international standards. Thus, this study suggesting that the fatty acid profile of Desmodesmus sp. VV2 attained under optimized media conditions would be suitable for biodiesel production for future energy demand.

Comparative study of different catalysts mediated FAME conversion from macroalga Padina tetrastromatica biomass and hydrothermal liquefaction facilitated bio-oil production.

Marine macroalgae offer an endurable source of renewable biomass, which do not require cultivable area, fertilizers for cultivation for bioproducts production. In this study, marine brown macroalga Padina tetrastromatica as an alternate sustainable feedstock for the production of liquid fuels. Padina tetrastromatica biomass was collected from Mandapam; the coastal region of Rameswaram, Tamil Nadu, India. and the algal oil was extracted using sequential extractions using various solvents. Petroleum ether (PE) and dichloromethane (DCM) solvent fractions were found to have high lipids and further utilized for biodiesel production, wherein four different heterogeneous nanocatalysts (TiO2, Bio-Fe, GO, and MgO) and commercial homogeneous catalysts (HCl and NaOH) were employed for the transesterification. High fatty acid methyl ester (FAME) recovery (92.3%) was achieved from TiO2 mediated transesterification than the other conventional catalysts. Further, the conversion of algal biomass into bio-oil and by-products was carried out using hydrothermal liquefaction (HTL). Subsequently, the compounds were characterized by FT-IR and GC-MS analysis. The quality parameters of liquid biofuels were examined and they are in accordance with the international fuel standards. Thus, brown macroalga Padina tetrastromatica may be considered as an alternate feedstock for biofuel and other bioproducts production and TiO2 would be a suitable catalyst for the conversion of FAME.