[A useful panel in proliferative urothelial lesions: an analysis of cytokeratin 20, p53, CD44 and Ki-67 antigens]. / Panel di ausilio diagnostico nella patologia proliferativa uroteliale: studio degli antigeni citocheratina 20, p53, CD44 e Ki-67.

Urothelium can show several pseudoneoplastic lesions. Mimic lesions diagnosis is therapeutically and prognostically critical but it is often difficult on the basis of morphology alone in bladder biopsy specimen. We studied in a retrospective way an immunohistochemical panel that helps in this differential diagnosis. The panel includes antibodies against cytokeratin 20 (CK20), CD44, Ki67 (MIB-1) e p53 and we applied it to 31 cases of non-neoplastic urothelium (26 urothelial biopsies with reactive atypia and 5 normal urothelium) and 50 cases of urothelial neoplasia where we analized the distribution of the markers over the urothelium. We obtained two different "standard" results, for neoplastic and non-neoplastic urothelium. We were able to prove that this panel is useful, non expensive tool in the differential diagnosis of urothelial proliferative lesions.

Fine needle aspiration cytology of the breast: a statistical evaluation of accuracy based on 1,080 cases.

Fine needle aspiration cytology (FNAC) of the breast is a minimally invasive and low-cost method. In this study we reviewed our experience on a total of 1,080 cases. Sensitivity, specificity and predictive value were calculated. The causes of error were examined and we concluded that accuracy improves when aspirations are done by the cytopathologist himself and fixing and staining procedures are immediately performed in a specialized laboratory.