1.
Am J Trop Med Hyg
; 89(4): 721-723, 2013 Oct.
Article
in English
| MEDLINE
| ID: mdl-23980132
ABSTRACT
We have modified an existing semi-nested multiplex polymerase chain reaction (PCR) by adding one Plasmodium knowlesi-specific nested PCR, and validated the latter against laboratory and clinical samples. This new method has the advantage of being relatively affordable in low resource settings while identifying the five human Plasmodium species with a three-step PCR.