Effects of cactus pear clone harvest seasons and times on the physicochemical and technological properties of resulting mucilage and biopolymeric films.

Cactus pear cladodes, clones 'Miúda' (MIU) and 'Orelha de Elefante Mexicana' (OEM) were harvested at 6 am and 8 pm during the rainy-dry, dry and rainy seasons to evaluate the effect of type of clone and harvest seasons on the physicochemical and technological properties of mucilage as well as the optical, physicochemical, mechanical, thermal and microstructural characteristics of the films obtained. The mucilage of the OEM clone presented a higher content of phenolic compounds, compared to the Nopalea genus, regardless of the season and time of harvest. Furthermore, the dry period resulted in higher carbohydrate levels, regardless of the harvest time. The biopolymeric films produced from the OEM clone harvested in the rainy season and rainy-dry transition showed darker color, better mechanical properties, water barrier, compact microstructure and thermal stability when compared to the MIU clone. Furthermore, harvesting at 6 am provided improvements in the mechanical conditions, permeability and thermal stability of the films of both types of clones studied. These results showed strong environmental modulation, naturally incorporating important macromolecules such as carbohydrates and phenolic compounds, used in the industry in the production of nutraceutical foods, into the mucilage. Furthermore, harvesting cladodes at 6 am in the rainy and transitional (rainy-dry) periods provided better quality biopolymeric films and/or coatings.

Synthesis of Iron Oxides and Influence on Final Sizes and Distribution in Bacterial Cellulose Applications.

Iron oxide nanoparticles have been investigated due to their suitable characteristics for diverse applications in the fields of biomedicine, electronics, water or wastewater treatment and sensors. Maghemite, magnetite and hematite are the most widely studied iron oxide particles and have ferrimagnetic characteristics. When very small, however, these particles have superparamagnetic properties and are called superparamagnetic iron oxide nanoparticles (SPIONs). Several methods are used for the production of these particles, such as coprecipitation, thermal decomposition and microemulsion. However, the variables of the different types of synthesis must be assessed to achieve greater control over the particles produced. In some studies, it is possible to compare the influence of variations in the factors for production with each of these methods. Thus, researchers use different adaptations of synthesis based on each objective and type of application. With coprecipitation, it is possible to obtain smaller, more uniform particles with adjustments in temperature, pH and the types of reagents used in the process. With thermal decomposition, greater control is needed over the time, temperature and proportion of surfactants and organic and aqueous phases in order to produce smaller particles and a narrower size distribution. With the microemulsion process, the control of the confinement of the micelles formed during synthesis through the proportions of surfactant and oil makes the final particles smaller and less dispersed. These nanoparticles can be used as additives for the creation of new materials, such as magnetic bacterial cellulose, which has different innovative applications. Composites that have SPIONs, which are produced with greater rigour with regards to their size and distribution, have superparamagnetic properties and can be used in medical applications, whereas materials containing larger particles have ferromagnetic applications. To arrive at a particular particle with specific characteristics, researchers must be attentive to both the mechanism selected and the production variables to ensure greater quality and control of the materials produced.

Gamma radiation effect on the chemical, mechanical and thermal properties of PCL/MCM-48-PVA nanocomposite films.

In this work, poly (vinyl alcohol) (PVA) was employed to produce a Mesoporous Composition of Matter-48 Modified (MCM-48-M or MCM-48-PVA). After surface modification, MCM-48-M was used to produce nanocomposite (NC) films with polycaprolactone (PCL) as a matrix at room temperature. PCL and MCM-48 nanoparticles (NPs) were chosen due to their great biocompatibility and low toxicity. However, MCM-48-M is more compatible with PCL than MCM-48. NC films were sterilized by gamma radiation with a dose of 25 kGy and characterized by experimental techniques to investigate their chemical, mechanical (tensile) and thermal properties. Scanning electron microscopy (SEM) and transmission electronic microscopy (TEM) results indicated that MCM-48-M exhibited a random distribution in the PCL matrix. The PCL chemical structure was preserved in NC films as described by Fourier transform infrared (FT-IR) spectroscopy as well as the tensile and thermal properties of NC films. FT-IR and thermogravimetric analysis (TGA) results showed surface modification. X-ray diffraction (XRD) and differential scanning calorimetry (DSC) showed that crystalline symmetries were preserved and the crystallinity of NC films had small variations in all samples before and after irradiation, respectively. But, our results did not indicate major changes showing that this method is successful for the sterilization of PCL/MCM-48-PVA NC films.

Efficient microbial cellulose/Fe3O4 nanocomposite for photocatalytic degradation by advanced oxidation process of textile dyes.

Fenton-type advanced oxidative processes (AOP) have been employed to treat textile dyes in aqueous solution and industrial effluent. The work focused on assisting the limitations still presented by the Fenton process regarding the use of suspended iron catalysts. Soon, a nanocomposite of bacterial cellulose (BC) and magnetite (Fe3O4) was developed. It has proven to be superior to those available in the literature, exhibiting purely catalytic properties and high reusability. Its successful production was verified through analytical characterization, while its catalytic potential was investigated in the treatment of different textile matrices. In initial tests, the photo-Fenton process irradiated and catalyzed by sunlight and BC/Fe3O4 discolored 92.19% of an aqueous mixture of four textile dyes. To improve the efficiency, the design of experiments technique evaluated the influence of the variables pH, [H2O2], and the number of BC/Fe3O4 membranes. 99.82% of degradation was obtained under optimized conditions using pH 5, 150 mg L-1 of H2O2, and 11 composite membranes. Reaction kinetics followed a pseudo-first-order model, effectively reducing the organic matter (COD = 83.24% and BOD = 88.13%). The composite showed low iron leaching (1.60 ± 0.08 mg L-1) and high stability. It was recovered and reused for 15 consecutive cycles, keeping the treatment efficiency at over 90%. As for the industrial wastewater, the photo-Fenton/sunlight/BC/Fe3O4 system showed better results when combined with the physical-chemical coagulation/flocculation process previously used in the industry's WWTP. Together they reduced COD by 77.77%, also meeting the color standards (DFZ scale) for the wavelengths of 476 nm (<3 m-1), 525 nm (<5 m-1), and 620 nm (<7 m-1). Thus, the results obtained demonstrated that employing the BC/Fe3O4 composite as an iron catalyst is a suitable alternative to materials employed in suspension. This is mainly due to the high catalytic activity and power of reuse, which will reduce treatment costs.

Magnetic Bacterial Cellulose Biopolymers: Production and Potential Applications in the Electronics Sector.

Bacterial cellulose (BC) is a biopolymer that has been widely investigated due to its useful characteristics, such as nanometric structure, simple production and biocompatibility, enabling the creation of novel materials made from additive BC in situ and/or ex situ. The literature also describes the magnetization of BC biopolymers by the addition of particles such as magnetite and ferrites. The processing of BC with these materials can be performed in different ways to adapt to the availability of materials and the objectives of a given application. There is considerable interest in the electronics field for novel materials and devices as well as non-polluting, sustainable solutions. This sector influences the development of others, including the production and optimization of new equipment, medical devices, sensors, transformers and motors. Thus, magnetic BC has considerable potential in applied research, such as the production of materials for biotechnological electronic devices. Magnetic BC also enables a reduction in the use of polluting materials commonly found in electronic devices. This review article highlights the production of this biomaterial and its applications in the field of electronics.

Original nanostructured bacterial cellulose/pyrite composite: Photocatalytic application in advanced oxidation processes.

The development of an original catalytic composite of bacterial cellulose (BC) and pyrite (FeS2) for environmental application was the objective of this study. Nanoparticles of the FeS2 were synthesized from the hydrothermal method and immobilized on the BC structure using ex situ methodology. In the BC, the FTIR and XRD analyzes showed the absorption band associated with the Fe-S bond and crystalline peaks attributed to the pyrite. Thus, the immobilization of the iron particles on the biopolymer was proven, producing the composite BC/FeS2. The use of the SEM technique also ratifies the composite production by identifying the fibrillar structure morphology of the cellulose covered by FeS2 particles. The total iron concentration was 54.76 ± 1.69 mg L-1, determined by flame atomic absorption analysis. TG analysis and degradation tests showed respectively the thermal stability of the new material and its high catalytic potential. A multi-component solution of textile dyes was used as the matrix to be treated via advanced oxidative processes. The composite acted as the catalyst for the Fenton and photo-Fenton processes, with degradations of 52.87 and 96.82%, respectively. The material proved stability by showing low iron leaching (2.02 ± 0.09 and 2.11 ± 0.11 mg L-1 for the respective processes). Thus, its high potential for reuse is presumed, given the remaining concentration of this metal in the BC. The results showed that the BC/FeS2 composite is suitable to solve the problems associated with using catalysts in suspension form.

Active packaging of corn starch with pectin extract and essential oil of Turmeric Longa Linn: Preparation, characterization and application in sliced bread.

The use of active packaging to reduce food waste has been a very effective alternative. An eminent concern is the use of plastic materials of petroleum origin and toxic additives in the processing of these packages. Thus, the focus on the use of biodegradable and natural raw materials that minimize waste generation and promote greater consumer safety has been preferable. The objective of the research was to investigate the effects of turmeric essential oil (TEO) on corn starch and pectin extract films manufactured by solution casting method. The antioxidant and antimicrobial potential of the oil was confirmed by the tests: antimicrobial diffusion disk, determination of the content of phenolic compounds and antioxidant activity by the DPPH and FRAP method. The chromatographic analysis confirmed the presence of active chemical constituents such as Turmerone, Ar-Turmerone and ß-Turmerone. The results showed that the oil promoted a change in the color of the films, increased mechanical strength and reduced flexibility, keeping transparency, solubility, WVP and thermal stability unchanged. In the direct application test of the film as packaging for sliced bread, no visible contamination was detected during the nine weeks of analysis. Therefore, the active film with 3 % TEO was shown to be a viable solution for manufacturing biodegradable and safe active films that can be applied as food packaging.

Design of a Naturally Dyed and Waterproof Biotechnological Leather from Reconstituted Cellulose.

Consumerism in fashion involves the excessive consumption of garments in modern capitalist societies due to the expansion of globalisation, especially at the beginning of the 21st Century. The involvement of new designers in the garment industry has assisted in creating a desire for new trends. However, the fast pace of transitions between collections has made fashion increasingly frivolous and capable of generating considerable interest in new products, accompanied by an increase in the discarding of fabrics. Thus, studies have been conducted on developing sustainable textile materials for use in the fashion industry. The aim of the present study was to evaluate the potential of a vegan leather produced with a dyed, waterproof biopolymer made of reconstituted bacterial cellulose (BC). The dying process involved using plant-based natural dyes extracted from Allium cepa L., Punica granatum, and Eucalyptus globulus L. The BC films were then shredded and reconstituted to produce uniform surfaces with a constant thickness of 0.10 cm throughout the entire area. The films were waterproofed using the essential oil from Melaleuca alternifolia and wax from Copernicia prunifera. The characteristics of the biotechnological vegan leather were analysed using scanning electron microscopy (SEM), thermogravimetric analysis (TGA), flexibility and mechanical tests, as well as the determination of the water contact angle (°) and sorption index (s). The results confirmed that the biomaterial has high tensile strength (maximum: 247.21 ± 16.52 N) and high flexibility; it can be folded more than 100 times at the same point without breaking or cracking. The water contact angle was 83.96°, indicating a small water interaction on the biotextile. The results of the present study demonstrate the potential of BC for the development of novel, durable, vegan, waterproof fashion products.

Development of hydrophobic polyurethane/castor oil biocomposites with agroindustrial residues for sorption of oils and organic solvents.

Since pollution of aquifer sources by organic compounds is a threat to the environment and humans, technology's constant advancement in developing sustainable adsorbents using renewable resources with hydrophobic characteristics has become increasingly attractive. In this study, we developed and characterized castor oil-based polyurethane biocomposites reinforced with malt and/or acerola residues for adsorption/separation of various organic oils/solvents. The residues evidenced biocomposite flexibility, increase in adsorption capacity for all organic oils/solvents, with a maximum of 3257.8 ± 55.6% for chloroform in 60 min and hydrophobicity (144 ± 0.2°) in acidic, saline and alkaline environments, and when exposed to UV radiation. These biocomposites can be quickly and repeatedly recovered by simple hand-tightening and solvent release systems (10 times) and vacuum suction filtration (20.5 ± 0.9 s/10 mL). Through the pseudo-first order (PPO) and pseudo-second order (PSO) kinetic models, the engine oil adsorption capacity increased with time from 746.7 ± 77.4% (60 min) to 928.6 ± 16.7% (90 min). These bio-composites are prototypes to be used with malt and acerola bagasse residues, becoming a reference for future works, produced in a sustainable way, with high potential for application in treatment of effluents with organic pollutants.

Dyeing of bacterial cellulose films using plant-based natural dyes.

The aim of this work was to test the use of plant-based natural dyes on bacterial cellulose (BC) to add aesthetic value to dyed pellicles while maintaining the mechanical properties. Natural pigments from Clitoria ternatea L. and Hibiscus rosa-sinensis were tested. The commercial ARAQCEL RL 500 was also used for comparison purposes. The behavior of biocellulose regarding dye fixation, rehydration, tensile strength, and elasticity was evaluated in comparison to the dried biomaterial, showing that dyeing is a process that can be performed on hydrated BC. Dyeing the BC films through an innovative process maintained the crystallinity, thermal stability and mechanical strength of the BC and confirmed the compatibility of the membrane with the dyes tested, from the observed Scanning Electron Microscopy (SEM) morphology of nanofibers. Dyed biomaterial can be applied to various products, as confirmed by the results of the mechanical tests. As environmental awareness and public concern regarding pollution increase, the combination of natural dyes and BC pellicles can produce an attractive new material for the textile industry.

Reprocessing of Catheters Used for Coronary Angiography and Changes in the Polymeric Structures.

BACKGROUND: Catheters are extensively used for coronary angiographies. These devices are long, thin, and flexible tubes of variable diameters that are made of the following polymer compounds: polyamide, polyurethane and Teflon. The objectives of this study are to identify changes that occur in the polymeric structure of the catheters, including an analysis of microcracks, fissures, cuts, perforations, roughness, and porosity in the internal and external layers after the first, second, third, and seventh reprocessing. METHODS: Five catheters of the same brand and manufacturer were selected for each analysis, except for the new and seventh catheters, which had only one sample. The catheters that we analyzed included new catheters, catheter reprocessed once (1st), catheters reprocessed twice (2nd), catheters reprocessed three times (3rd), and catheters reprocessed up to seven times (7th). Samples were cut into small pieces and the scanning electron microscope and infrared spectroscopy were used for analysis. RESULTS: Scanning electron microscopy (SEM) of reprocessed catheters revealed rougher surface with porosity, stainless steel wires, peeling of the inner layers, micropores, small holes, disordered cracks, cracks, grooves, microcracks, granular appearance, dots whitish, diffuse microcracks, desquamation, diffuse recesses, depressions, protrusions and deep crevices. Infrared spectroscopy suggested a possible change in the chemical structures of the polymer. There was a directly proportional relationship between the reprocessing number and the carbonyl ratio. CONCLUSIONS: The combined SEM and infrared analyses reveal that reprocessing damages the integrity of coronary angiography catheters. Therefore, the reprocessing of such catheters is not recommended.

Quitosana, óxido de zinco e óleo essencial de pimenta rosa: propriedades e potencialidades de aplicações / Chitosan, zinc oxide and pink pepper essential oil: potential properties and applications

Objetivo: descrever propriedades e aplicações potenciais da quitosana, óxido de zinco e óleo essencial de pimenta rosa. Método: estudo descritivo, tipo informativo, mediante o levantamento do universo da produção científica. Resultados: a quitosana é um polímero que possui características tais como biodegrabilidade, biocompatibilidade, atoxicidade, baixo custo, produto natural e existe em grande quantidade na natureza. É suscetível de ser manipulada nos formatos de lâminas, filmes, fibras e géis. É uma molécula extremamente funcional exercendo ação mucoadesiva, anticoagulante, imunoestimulante, cicatrizante, antitumoral, hemostática, hipolipêmica, antimicrobiana, dentre outras. O óxido de zinco é um antimicrobiano com extraordinária eficácia bactericida e fungicida. Estudos têm investigado o óleo essencial da pimenta rosa e identificado que ele possui atividade diurética, adstringente, antidiarreica, anti-inflamatória, cicatrizante, bactericida, antiviral, analgésica, sedativa, expectorante, antipirética, vermífuga e antisséptica. Conclusão: a quitosana, o óxido de zinco e o óleo essencial de pimenta rosa apresentam, individualmente ou em conjunto, potencialidades para aplicações em áreas tais como embalagens ativas, tratamento de água, curativos e outras, em consequência da competência para minimizar ou erradicar a proliferação de micro-organismos patógenos.(AU)

Production of Bacterial Cellulose by Gluconacetobacter hansenii Using Corn Steep Liquor As Nutrient Sources.

Cellulose is mainly produced by plants, although many bacteria, especially those belonging to the genus Gluconacetobacter, produce a very peculiar form of cellulose with mechanical and structural properties that can be exploited in numerous applications. However, the production cost of bacterial cellulose (BC) is very high to the use of expensive culture media, poor yields, downstream processing, and operating costs. Thus, the purpose of this work was to evaluate the use of industrial residues as nutrients for the production of BC by Gluconacetobacter hansenii UCP1619. BC pellicles were synthesized using the Hestrin-Schramm (HS) medium and alternative media formulated with different carbon (sugarcane molasses and acetylated glucose) and nitrogen sources [yeast extract, peptone, and corn steep liquor (CSL)]. A jeans laundry was also tested. None of the tested sources (beside CSL) worked as carbon and nutrient substitute. The alternative medium formulated with 1.5% glucose and 2.5% CSL led to the highest yield in terms of dry and hydrated mass. The BC mass produced in the alternative culture medium corresponded to 73% of that achieved with the HS culture medium. The BC pellicles demonstrated a high concentration of microfibrils and nanofibrils forming a homogenous, compact, and three-dimensional structure. The biopolymer produced in the alternative medium had greater thermal stability, as degradation began at 240°C, while degradation of the biopolymer produced in the HS medium began at 195°C. Both biopolymers exhibited high crystallinity. The mechanical tensile test revealed the maximum breaking strength and the elongation of the break of hydrated and dry pellicles. The dry BC film supported up to 48 MPa of the breaking strength and exhibited greater than 96.98% stiffness in comparison with the hydrated film. The dry film supported up to 48 MPa of the breaking strength and exhibited greater than 96.98% stiffness in comparison with the hydrated film. The values obtained for the Young's modulus in the mechanical tests in the hydrated samples indicated low values for the variable rigidity. The presence of water in the interior and between the nanofibers of the hydrated BC only favored the results for the elasticity, which was 56.37% higher when compared to the dry biomaterial.

Evaluation of the types of starch for preparation of LDPE/starch blends

This study evaluated in relation the growth, and the amylolytic activity of mixed and isolated cultures of Phanerochaete chrysosporium and Talaromyces wortmanni on different types of starch. The thermal and mechanical properties in polyethylene/starch blends (proportion: 80/20 (w/w) before and after inoculation of the mixed cultures were evaluated. The regular starch Amidex 3 and the modified starch Fox5901 stood out in relation to the cellular growth and production of the amylase enzyme. In spite of the short time that the blends were exposed to the fungi, the microorganisms promoted physical and chemical changes in the structure of the blend, modifying its thermal and mechanical properties. The alteration of the degree of crystallinity and mechanical properties of the blends could be indications of the modification caused by the biodegradation process.

Nesse trabalho foi realizado um estudo sobre diferentes tipos de amido quanto ao crescimento, e a atividade amilolítica de culturas mistas e isoladas dos fungos Phanerochaete chrysosporium e Talaromyces wortmannii. Avaliaram-se também as propriedades térmicas e mecânicas das blendas de polietileno/amido anfótero (na proporção 80/20 (m/m)) antes e apos a inoculação das culturas mistas desses fungos.O amido regular Amidex 3 e o amido modificado Fox5901 foram os que se destacaram quanto ao crescimento celular e produção da enzima amilase. Apesar do pouco tempo de exposição dos filmes com os fungos, pode-se concluir que os microrganismos promovem mudanças físicas e químicas na estrutura da blenda, modificando suas propriedades térmicas e mecânicas. A alteração do grau de cristalinidade e das propriedades mecânicas das blendas podem ser indícios da modificação provocada pelo processo de biodegradação.