ABSTRACT
An association of HTLV-I virus with myelomonocytic leukemia was established by serologic and molecular-biologic procedures. Antibodies to one or two HTLV-I proteins were identified in 10 out of 30 patients with myelomonocytic leukemia; both antibodies to certain HTLV-I proteins and an integrated provirus were detected in 3 cases. Upon examination of donor blood, antibodies to one or two HTLV-I proteins were found in 0.8% cases, but only 0.3% of the examined donors were found positive to HTLV-I. This finding points to the presence of said virus in the population of Latvia.
Subject(s)
HTLV-I Infections/complications , Leukemia, Myeloid/microbiology , Aged , Female , HTLV-I Antibodies/analysis , HTLV-I Infections/immunology , Humans , Karyotyping , Leukemia, Myeloid/immunology , Leukemia, Myelomonocytic, Acute/microbiology , Leukemia, Myelomonocytic, Chronic/microbiology , Male , Middle AgedABSTRACT
Molecular cloning has been done of DNA isolated from leukocytes of a patient with T-cell prolymphocytic leukemia. Three HTLV-I-containing recombinant clones have been obtained and their restriction-hybridization analysis performed. None of the clones contained a complete HTLV-I provirus. In all clones, there have been detected nucleotide sequences corresponding to the provirus env-pX-3' LTR region.
Subject(s)
Human T-lymphotropic virus 1/genetics , Leukemia, Prolymphocytic/microbiology , Leukemia, T-Cell/microbiology , Cloning, Molecular , Humans , Nucleic Acid HybridizationABSTRACT
26 base long deoxyribonucleotide complementary to the lower part of the Central Conserved Region of chrysanthemum stund viroid (CSV) was used for synthesis of the first strand cDNA. The cDNA was cloned into plasmid vector pUC19 and the primary structure was determined. Cloned, full length cDNA was used as hybridisation probe for detection of CSV. It was possible to detect about 26 pg of purified CSV RNA immobilized on nitrocellulose filters using 32P-labeled probe. In the case of biotinylated probe it was possible to detect about 26 pg of purified CSV RNA visualizing results by streptavidin-alkaline phosphatase conjugates. It has been shown that such a cloned cDNA can be used for wide scale detection of CSV.
Subject(s)
Chrysanthemum cinerariifolium/microbiology , DNA, Viral/genetics , DNA/genetics , Plant Viruses/genetics , Plasmids , Virion/genetics , Base Sequence , Cloning, Molecular , Genes, Viral , Molecular Sequence Data , Nucleic Acid Hybridization , Virion/isolation & purificationABSTRACT
Serologic and molecular biologic techniques were used to identify association of HTLV-1 with adult T-cell leukemia/lymphoma as well as with B-cell leukemia. HTLV-1 markers (antibodies and integrated provirus) were identified in all lympho- and myeloproliferative diseases, while integrated provirus genome--in T-cell population only.
Subject(s)
Human T-lymphotropic virus 1 , Leukemia, Lymphocytic, Chronic, B-Cell/diagnosis , Leukemia-Lymphoma, Adult T-Cell/diagnosis , Proviruses , Diagnosis, Differential , HTLV-I Antibodies/analysis , Humans , Myeloproliferative Disorders/diagnosis , Preleukemia/diagnosisSubject(s)
Adenoviridae/genetics , Cell Transformation, Neoplastic , Cell Transformation, Viral , Deoxyribonucleases, Type II Site-Specific , Oncogenes , Adenoviridae/physiology , Animals , Cattle , Cell Line , Clone Cells , Cricetinae , DNA Restriction Enzymes/metabolism , DNA, Viral/analysis , Deoxyribonuclease BamHI , Female , Pregnancy , Rats , Virus ReplicationABSTRACT
Cloned cultures of rat embryo cells transformed by BA-3 DNA and those transformed by the virus in hamsters were obtained. It was found that these cultures contain T-antigen and do not differ in oncogenicity for hosts. The adenovirus type 5 DNA transformed cells of the same primary culture of rat embryo fibroblasts produced no tumors in syngeneic newborn rats. The role of the viral genome in oncogenicity of the cells transformed by it is discussed.
