[Mechanism of action of remantadine hydrochloride on Sindbis virus reproduction: inhibition of virus penetration into the cell]. / O mekhanizme deistviia solianokislogo remantadina na reproduktsiiu virusa Sindbis: ingibirovanie protsessa penetratsii virusa v kletku.

The effect of rimantadine and ammonium chloride on the RNA synthesis of Sindbis virus, added in different times of infection cycle, was studied. When compounds were presented in period of adsorption and first hour post-infection, the maximal inhibitory effect was found. The step blocked by rimantadine was identified as uncoating process (deprotainization). This connection was confirmed by the finding that rimantadine markedly reduced the change of the virus RNA into the nuclease-sensitive form.

[Interferon-inducing and antiviral activity of maleic anhydride copolymers]. / Interferonindutsiruiushchaia i protivovirusnaia aktivnost' sopolimerov maleinovogo angidrida.

Data on synthesis of new polymer compounds with potential interferon-inducing and antiviral activity are presented. Maleic anhydride (MA)-based alternating copolymers, in particular, copolymers of divinyl ether with MA containing five-member oxygen cycles in the chain, as well as furan copolymers with MA, were shown to be promising interferon inducers. The antiviral activity was found in copolymers based on allene hydrocarbons.

[Isolation of West Nile virus in the Southern Ukraine]. / Vydelenie virusa Zapadnogo Nila na iuge Ukrainskoi SSR.

A virus (strain No. 3266) was isolated from the blood and internal organs of a rook caught in May, 1980, in the territory of the Black Sea State Preserve of the Ukrainian Academy of Sciences (the Kherson region). The virus is pathogenic for 1--2-day-old mice by various routes of inoculation and for 3-week-old mice by the intracerebral route; it multiplies in chick embryos, in chick embryo fibroblast cultures without the cytopathic effect, and in continuous L cells and pig embryo kidney cells with definite cytopathic effect. The virus contains RNA and has a lipid-containing membrane; according to the results of filtration through Millipore filters, its size ranges from 50 to 100 nm. From the results of immunofluorescent studies and serological identification this strain has been classified as West Nile virus. The characteristics of the biological properties of this virus isolated in the Ukraine have first been described.

[Isolation of influenza strains identical to influenza virus A/Anglia/42/72 from semisynanthropic bird species in Rovno Province, the Ukrainian SSR]. / Vydelenie shtammov grippa identichnykh virusu grippa A/Angliia/42/72, ot polusinantropnykh vidov ptits v Rovenskoi oblasti USSR.

Two strains of influenza virus, A/wagtail/Ukraina/107/74 and A/dove/Ukraina/111/74, were isolated in virological studies of semisynanthropic species of birds shot near villages in the Rovno region (Western Ukraine). Both strains were found to be antigenically identical with influenza A/England/42/72 virus.

[Characteristics of the biological properties of a Kemerovo group arbovirus isolated in Transcarpathia]. / Kharakteristika biologicheskikh svoistv arbovirusa gruppy Kemerovo, vydelennogo v Zakarpat'e.

A strain of an arbovirus named Mircha was isolated from Ixodes ricinus L. ticks (30 incompletely engorged females) collected in May, 1972 from cows pastured in a forest near the village of Mircha of the Velikobereznyansky district of the Zakarpathye region of the Ukrainian SSR. The strain was found to be pathogenic for newborn white mice and rats by the intracerebral and intraperitoneal routes and for chick embryos inoculated into the yolk sac, to replicate in chick embryo fibroblast culture producing a marked cytopathic effect to be relatively stable to ethyl ether and sodium deoxylate. On the basis of the results of serological identification (CFT) it was classified into arboviruses of the Kemerovo group, the antigenic subgroup Kemeroxo-Tribec. The factors of natural focality are described briefly, and the results of the study of virus persistence in adult white mice, rats, and guinea pigs and of its immunodepressive effect are presented.

[Isolation of Bhanja arbovirus from Boophilus decoloratus ticks in Cameroon]. / Vydelenie arbovirusa Bkhandzha iz kleshchei Boophilus decoloratus v Kamerune.

A strain of virus designated YaK-10 was isolated from Boophilus decoloratus ticks (10 specimens) collected in March 1971 from cattle pastured in savanna near Garoy (United Cameroun Republic) by the intracerebral inoculation of suckling mice at the Pasteur Institute in Cameroun. The results of investigations of the biological properties of the strain showed it to be pathogenic for 1-3-day-old suckling mice by the intracerebral and intraperitoneal routes, for 3-week-old mice by the intracerebral route and to be nonpathogenic for adult mice. The incubation period was 5-7 days. The virus multiplies in chick embryos producing death of the majority of eggs and in chick embryo fibroblast cultures with a weak cytopathic effect, and it is sensitive to ether and sodium deoxycholate, passes Millipore filters with pore diameter of 220 nm and has no hemagglutinating properties. Studies of the antigenic properties of the virus by CF and neutralization tests showed it to be identical with Dakar D-9540 VIRUS (Bhanja).