ABSTRACT
The main hemodynamic and oxygen supply parameters of the isolated rat heart (128 experiments) were measured during its perfusion with human donor red cells differing by storage duration and kind of hemopreservatives. The degree of the reduction in efficacy of oxygen release to myocardial tissue and the speed of the release restoration depended both on the red cell storage time and the preservative composition. The authors advocate a new preservative citroglucophosphate with adenine as it proved superior to all the preservatives studied.
Subject(s)
Blood Preservation , Erythrocytes/metabolism , Myocardium/metabolism , Oxygen Consumption , Oxygen/blood , Animals , Efficiency/physiology , Erythrocyte Transfusion , Humans , In Vitro Techniques , Perfusion , Rats , Time FactorsABSTRACT
Seven series of experiments (68 tests) were conducted on an isolated rat heart with the functioning left ventricle during its coronary vessels perfusion with suspension of human erythrocytes of different storage terms preserved in the solution containing citroglucophosphate with adenine. The main parameters of hemodynamics and oxygen regimen were evaluated. The results of the study have shown that washed human donor erythrocytes preserved in the above solution after 1, 7, 14, 21 and 28 days of storage present a highly efficient gas-carrying medium under conditions of acute anemic hypoxia.
Subject(s)
Blood Preservation/methods , Cryopreservation/methods , Erythrocytes/physiology , Heart Arrest, Induced , Hypoxia/prevention & control , Models, Cardiovascular , Myocardium/metabolism , Oxygen Consumption/physiology , Oxygen/blood , Adenine/administration & dosage , Adenine/pharmacology , Animals , Anticoagulants/administration & dosage , Citrates/administration & dosage , Citrates/pharmacology , Cryoprotective Agents , Erythrocytes/drug effects , Glucose/administration & dosage , Glucose/pharmacology , Humans , Male , Oxygen Consumption/drug effects , Perfusion/methods , Rats , Rats, Inbred Strains , Solutions , Time FactorsABSTRACT
The isolated rat heart with the functioning left ventricle was used to measure the main parameters of hemodynamics and oxygen supply (60 experiments). Perfusion of the coronary vessels of the heart was performed with washed human red blood cells preserved for varying times (for 1, 7, 10, 14 and 21 days), stabilized on citroglucophosphate. The authors succeeded for the first time in demonstrating under artificially made conditions where the sole variable responsible for oxygen supply of the isolated organ was hemoglobin affinity for oxygen that there was no difference in the function of the isolated heart perfused with human red blood cells preserved for 7, 10 and 14 days as compared with freshly obtained ones. During perfusion of the heart with red blood cells preserved for an insignificant period of time (for 7 and 10 days), no physiological signs evidencing the impairment of oxygen supply to myocardial tissues were revealed.
