Avalanche Photodiodes and Silicon Photomultipliers of Non-Planar Designs.

Conventional designs of an avalanche photodiode (APD) have been based on a planar p-n junction since the 1960s. APD developments have been driven by the necessity to provide a uniform electric field over the active junction area and to prevent edge breakdown by special measures. Most modern silicon photomultipliers (SiPM) are designed as an array of Geiger-mode APD cells based on planar p-n junctions. However, the planar design faces an inherent trade-off between photon detection efficiency and dynamic range due to loss of an active area at the cell edges. Non-planar designs of APDs and SiPMs have also been known since the development of spherical APDs (1968), metal-resistor-semiconductor APDs (1989), and micro-well APDs (2005). The recent development of tip avalanche photodiodes (2020) based on the spherical p-n junction eliminates the trade-off, outperforms the planar SiPMs in the photon detection efficiency, and opens new opportunities for SiPM improvements. Furthermore, the latest developments in APDs based on electric field-line crowding and charge-focusing topology with quasi-spherical p-n junctions (2019-2023) show promising functionality in linear and Geiger operating modes. This paper presents an overview of designs and performances of non-planar APDs and SiPMs.

Assessing the Sensitivity of Plasmopara halstedii Isolates to Mefenoxam through Host Responses.

Downy mildew caused by Plasmopara halstedii is responsible for significant economic losses in cultivated sunflowers. Field isolates of sunflower downy mildew resistant to mefenoxam, a previously effective active ingredient against the pathogen, have been found across Europe. The main goal of this study was to assess the sensitivity of P. halstedii isolates to mefenoxam through host responses to infection, such as symptoms measured by disease severity and growth reduction, and host tissue reactions, such as hypersensitive reaction and necrosis of invaded cells. Sunflower seeds were treated with Apron XL 350 FS at the European registered rate (3 mg/kg seeds). Seedlings were inoculated using the soil drench method with eight Hungarian P. halstedii isolates. Disease rates and plant heights were measured twice. Histological examinations of cross-sections of sunflower hypocotyls were performed using a fluorescence microscope. In our study, cluster analyses of sunflowers based on macroscopic and microscopic variables showed differentiation of groups of mefenoxam-treated sunflowers inoculated with different P. halstedii isolates. We first revealed a clear difference in host responses of mefenoxam-treated susceptible sunflowers. In addition, examining tissue reactions (e.g., hypersensitive reaction, necrosis) seems more accurate to estimate the sensitivity of P. halstedii isolates to mefenoxam than macroscopic symptoms.

Economic damages from Hurricane Sandy attributable to sea level rise caused by anthropogenic climate change.

In 2012, Hurricane Sandy hit the East Coast of the United States, creating widespread coastal flooding and over $60 billion in reported economic damage. The potential influence of climate change on the storm itself has been debated, but sea level rise driven by anthropogenic climate change more clearly contributed to damages. To quantify this effect, here we simulate water levels and damage both as they occurred and as they would have occurred across a range of lower sea levels corresponding to different estimates of attributable sea level rise. We find that approximately $8.1B ($4.7B-$14.0B, 5th-95th percentiles) of Sandy's damages are attributable to climate-mediated anthropogenic sea level rise, as is extension of the flood area to affect 71 (40-131) thousand additional people. The same general approach demonstrated here may be applied to impact assessments for other past and future coastal storms.

Electrochemical Detection of Small Molecule Induced Pseudomonas aeruginosa Biofilm Dispersion.

A simple electrochemical assay to monitor the dispersion of Pseudomonas aeruginosa PA01 biofilm is described. Pyrolytic graphite (PG) electrodes were modified with P. aeruginosa PA01 using layer-by-layer (LbL) methods. The presence of the bacteria on the electrodes was directly monitored using square wave voltammetry (SWV) via the electrochemical reduction of electroactive phenazine compounds expressed by the bacteria, which indicate the presence of biofilm. Upon treatment of bacteria-modified electrodes with a 2-aminoimidazole (2-AI) derivative with known Pseudomonas anti-biofilm properties, the bacteria-related electrochemical reduction peaks decreased in a concentration dependent manner, indicating dispersal of the biofilm on the electrode surface. A similar 2-AI compound with negligible anti-biofilm activity was used as a comparative control and produced muted electrochemical results. Electrochemical responses mirrored previously established bioassay-derived half maximal inhibition concentration (IC50) and half maximal effective concentration (EC50) values.. Biofilm dispersal detection via the electrochemical response was validated by monitoring crystal violet absorbance after its release from electrode confined P. aeruginosa biofilm. Mass spectrometry data showing multiple redox active phenazine compounds are presented to provide insight into the surface reaction complexity. Overall, we present a very simple assay to monitor the anti-biofilm activity of compounds of interest.

New Magnetostrictive Transducer Designs for Emerging Application Areas of NDE.

Magnetostrictive transduction has been widely utilized in nondestructive evaluation (NDE) applications, specifically for the generation and reception of guided waves for the long-range inspection of components such as pipes, vessels, and small tubes. Transverse-motion guided wave modes (e.g., torsional vibrations in pipes) are the most common choice for long-range inspection applications, because the wave motion is in the plane of the structure surface, and therefore does not couple well to the surrounding material. Magnetostrictive-based sensors for these wave modes using the Wiedemann effect have been available for several years. An alternative configuration of a sensor for generating and receiving these transverse-motion guided waves swaps the biasing and time-varying magnetic field directions. This alternative design is a reversed Wiedemann effect magnetostrictive transducer. These transducers exhibit a number of unique features compared with the more conventional Wiedemann sensor, including: (1) the use of smaller rare earth permanent magnets to achieve large, uniform, and self-sustained bias field strengths; (2) the use of more efficient electric coil arrangements to induce a stronger time-varying magnetic field for a given coil impedance; (3) beneficial non-linear operating characteristics, given the efficiency improvements in both magnetic fields; and (4) the ability to generate unidirectional guided waves when the field arrangement is combined with a magnetically soft ferromagnetic strip (patch). Reversed Wiedemann effect magnetostrictive transducers will be presented that are suitable for different inspection applications, one using electromagnetic generation and reception directly in a ferromagnetic material, and another design that integrates a magnetostrictive patch to improve its efficiency and enable special operating characteristics.

Design and formulation of polyplexes based on pluronic-polyethyleneimine conjugates for gene transfer.

Previously, we reported the evaluation of several polyplex-based gene delivery systems with respect to their effectiveness, toxicity, and cell type dependence in vitro. One system, P123-g-PEI(2K), a cationic graft block copolymer, is of particular interest as it has been demonstrated to successfully deliver genetic material to murine liver following systemic delivery [Nguyen, H. K., Lemieux, P., Vinogradov, S. V., Gebhart, C. L., Guerin, N., Paradis, G., Bronich, T. K., Alakhov, V. Y., and Kabanov, A. V. (2000) Evaluation of Polyether-Polyethyleneimine Graft Copolymers as Gene Transfer Agents. Gene Ther. 7, 126-138 (1)]. The P123-g-PEI(2K) system requires nonmodified Pluronic P123 as an excipient to stabilize the dispersion. The purpose of the current work was to more closely characterize this system, to assess the role of each component of the system to the overall transfection process. We evaluated particle size, stability, and resistance to nuclease degradation. In addition, cellular uptake and localization of plasmid, as well as transgene expression, were evaluated following in vitro transfection of prostate cancer cells (PC-3) with various individual components of the system. Nonmodified Pluronic alone did not significantly enhance DNA uptake, transgene expression, or DNase protection. Therefore, we conclude that nonmodified Pluronic acted primarily by optimizing the size of the polyplex. Furthermore, though this system displays several characteristics thought desirable of a nonviral gene delivery system, these studies did discriminate a potential limitation of this system for in vivo applications, namely, the insufficient level of protection of plasmid DNA from nuclease degradation. This may limit the effective dose delivered, as well as limiting the effective circulation time. These studies provide vital information that will guide modification of this system to enhance the current in vivo profile.

Pluronic block copolymers: novel functional molecules for gene therapy.

Pluronic block copolymers are recognized pharmaceutical excipients listed in the US and British Pharmacopoeia. They have been used extensively in a variety of pharmaceutical formulations including delivery of low molecular mass drugs and polypeptides. This review describes novel applications of Pluronic block copolymers in gene therapy. In particular, these molecules can modify the biological response during gene therapy in the skeletal muscle, resulting in an enhancement of the transgene expression as well as an enhancement of the therapeutic effect of the transgene. Furthermore, Pluronic block copolymers are versatile molecules that can be used as structural elements of the polycation-based gene delivery systems (polyplexes). Based on these studies, the use of block copolymers in gene delivery is a promising area of research, in which new and important developments are expected.