[Alcoholic hyalin and interstitial filaments as markers of alcoholic damage of internal organs]. / Alkogol'nyi gialin i promezhutochnye filamenty kak markery alkogol'nogo porazheniia vnutrennikh organov.

Biopsies of the liver, stomach, pancreas, small salivary glands, lung of patients with chronic alcoholism were studied morphologically including ultrastructural analysis. The formation of fibrillar alcoholic hyalin in hepatocytes is the most characteristic ultrastructural feature of the liver alcoholic damage. Accumulation of intermediate filaments in the epithelial cells of the stomach, pancreas, small salivary glands, stomach macrophages is a characteristic sign of the alcoholic damage to these organs. This disturbance of the structural organization of the cytoskeleton seems to reflect secretory insufficiency of the epithelium and failure of macrophagal function.

[Morphology and morphogenesis of chronic gastritis in patients with alcoholism]. / Morfologiia i morfogenez khronicheskogo gastrita u bol'nykh alkogolizmom.

The characteristics of all types of chronic gastritis in patients with chronic alcoholism is given. Alterations of the stomach mucous membrane in alcoholism of different duration are described in detail. The intermediate type filaments in the surface epithelium, cells of glands and macrophages are found which is reported to be a morphological manifestation of alcoholism.

[Intermediate filaments in the lung macrophages and endothelial cells in chronic alcoholism and suppurative-destructive lung diseases]. / Promezhutochnye filamenty v makrofagakh i éndotelial'nykh kletkakh legkogo u bol'nykh khronicheskim alkogolizmom i gnoino-destruktivnymi zabolevaniiami legkikh.

Accumulation of intermediate filaments in lung macrophages and endotheliocytes has been found in patients with chronic alcoholism and purulent destructive pulmonary diseases. Excess accumulation of cytoskeletal elements reflects cell dystrophy. The authors believe that impairment of macrophage function may favour the lingering of the suppurative process.

[Streptomycin-3"-phosphotransferases from streptomycin-resistant cells of Escherichia coli strains]. / Streptomitsin-3"-fosfotransferazy iz kletokh ustoichnvykh k streptomitsinu shtammov Escherichia coli.

Streptomycin-3"-phosphotransferases were isolated and purified from E. coli cells containing plasmids 836, pBS52 or R6K, which determine the microorganisms resistance towards streptomycin and dihydrostreptomycin. Phosphorylation of the 3"-hydroxylic group of dihydrostreptomycin was demonstrated by [13C]-NMR spectrometry. It was shown that streptomycin-3"-phosphotransferase, whose synthesis is determined by plasmid 836 (as well as by plasmid R6K), differs from the analogous enzyme, whose synthesis is operated by plasmid pBS52 in some properties, e. g. dependence of the initial reaction rate on concentrations of antibiotics and ATP, pH-optimum, sensitivity to the buffer ionic strength, stability, etc. Besides, the antiserum against streptomycin-3'-phosphotransferase detected by plasmid pBS52 does not produce cross immunological reactions with the other enzyme.

[Morphological variants of alcoholic hyalin and possible ways of its evolution]. / Morfologicheskie varianty alkogol'nogo gialina i vozmozhnye puti ego evoliutsii.

Possible ways of alcohol hyalin evolution were studied by repeated electron microscopic examinations of the livers of 2 patients with acute alcoholic hepatitis Four types of ultrastructure of alcoholic hyalin were found: parallely oriented fibrillae, randomly oriented fibrillae, fine granular and large granular (spotlike) substance. Examinations of the material from repeated biopsies showed fibrillar hyalin to be "young" and to turn into "old" hyalin of granular structure. The evolution of alcoholic hyalin from fibrillar to granular is accompanied by hepatocyte necrosis and leukotaxic effect.