Subject(s)
Uveitis/diagnosis , Animals , Child, Preschool , Diagnosis, Differential , Humans , Infant , Macaca fascicularisSubject(s)
Echovirus Infections/microbiology , Uveitis/microbiology , Animals , Enterovirus B, Human/pathogenicity , Haplorhini , Humans , Infant , VirulenceSubject(s)
Uveitis/immunology , Virus Diseases/immunology , Adolescent , Child , Child, Preschool , Humans , InfantABSTRACT
Four strains of ECHO-19 virus were isolated from a child with respiratory disease complicated with uveitis. A freshly isolated Rad/40 strain of ECHO-19 virus was found to produce a severe disease of the vascular coat of the eye (uveitis) in Javan Cercopithecus monkeys inoculated directly into the anterior chamber of the eye. Experimental uveitis is characterized by marked changes in the shape of the pupil, the lack of the pupil reaction to light, development of synechias, changes in the color of the iris. No uveitis developed after inoculation of the monkeys with the prototype Burke strain of ECHO-19 virus of culture fluid from uninoculated RD cell cultures (control). It is suggested that the freshly isolated strains of ECHO-19 virus (Rad/40 strain) could cause uveitis in the child under observation.
Subject(s)
Enterovirus B, Human/pathogenicity , Uveitis/microbiology , Animals , Animals, Newborn , Arvicolinae , Cytopathogenic Effect, Viral , Macaca fascicularis , Mice , Rats , Uveitis/pathology , Virus CultivationSubject(s)
Blood Preservation/methods , Erythrocytes/physiology , Glycerol/isolation & purification , Blood Preservation/instrumentation , Blood Transfusion , Cell Separation/methods , Centrifugation , Cryoprotective Agents/isolation & purification , Erythrocyte Transfusion , Freezing , Humans , Temperature , Time Factors , USSRABSTRACT
The use of a passive haemagglutination test (PHA) with a stable erythrocyte diagnostic preparation sensitized with herpes simplex virus for the detection of antibody to herpes simplex virus (HSV) in the lacrimal fluid of the patients is described. Specific antibody to HSV antigen was found in 68% of the lacrimal fluids taken from patients suffering from herpetic eye disease. In patients with superficial keratitis the antibodies were found more frequently and in higher titers than in deep involvements of the cornea. In patients with keratitis and keratouveitis of unknown etiology, antibody in tears was found in 23%, and in those with inflammatory diseases of nonherpetic etiology, in 14.2%. In clinically normal subjects and patients with non-inflammatory eye affections no HSV antibody could be found in tears. Geometric mean antibody titers in tears of patients with herpes simplex keratitis and keratouveitis were statistically significantly higher than in patients with other diagnoses. The diagnostic importance of HSV antibody found in tears is discussed.
Subject(s)
Eye Diseases/diagnosis , Keratitis, Dendritic/diagnosis , Virus Diseases/diagnosis , Adolescent , Adult , Aged , Antibodies, Viral/analysis , Child , Eye Diseases/immunology , Hemagglutination Tests , Humans , Keratitis/diagnosis , Keratitis, Dendritic/immunology , Middle Aged , Tears/immunology , Uveitis/diagnosis , Virus Diseases/immunologySubject(s)
Antibodies, Viral/analysis , Aqueous Humor/immunology , Hemagglutination Tests/methods , Influenza A virus/immunology , Tears/immunology , Adult , Child , Female , Humans , Male , Middle AgedABSTRACT
Influenza virus monoinfection and combined influenza and herpes simplex virus (HSV) infection of the eye was studied in rabbits. Influenza A/Hong Kong/1/68 (H3N2) virus caused a clinically overt disease of the eye only after inoculation into the eye chamber. The combined influenza and HSV infection of the eye induced severe iridocyclitis. The combined infection of the eye with influenza virus and HSV had a more severe course than HSV monoinfection. The occurence of influenza antibody in the lacrimal fluid in the presence of the antigen in eye tissues is of diagnostic importance in determining the aetiology of the disease.
