[Role of lectin (hlyA) in the hemolytic and hemagglutinating activity of Vibrio cholerae]. / Rol' lektina (hlyA) v gemoliticheskoi gemagglutiniruiushchei aktivnosti Vibrio cholerae.

Data on the nature of the substance which determines the structural gene hlyA in V. cholerae are presented. Computer analysis and experimental data on hemolysin preparations and V. cholerae strains testify that gene hlyA determines the synthesis of ricin-like galactose-specific lectin. Its lectin domain takes part in the lysis of sheep (but not rabbit!) red blood cells, as well as in the hemagglutinating capacity of non-toxigenic and toxigenic V. cholerae 569 B.

[Adhesive and other properties of Vibrio cholerae tcp+ ctx- isolated from environmental objects in the Rostov region in 2002]. / Adgezivnye i nekotorye drugie svoistva Vibrio cholerae tcp+ ctx-, izolirovannykh iz ob"ektov vneshnei sredy Rostovskoi oblasti v 2002 godu.

The adhesive, hemolytic and triacylglycerol lipase activity of 4 V. cholerae ctp+ ctx- cultures, sensitive to bacteriophage ctx+, isolated from the Don and sewage water were tested. All these cultures were found to induce hemolysis of sheep red blood cells in the Greig test in 2 hours, possessed triacylglycerol lipase activity, but had no adhesive properties. By these parameters--atoxigenicity and the absence of adhesive properties--the isolated V. cholerae strains were characterized as avirulent and epidemically safe.

[Experimental evaluation of efficacy of Lactobacilli in prophylaxis and treatment of cholera]. / Eksperimental'naia otsenka éffektivnosti laktobatsill dlia profilaktiki i lecheniia kholery.

Investigations on experimental models of cholera ("sealed" mice and suckling rabbits) demonstrated that previous daily oral administration of the ferment culture of Lactobacillus acidophilus BKM B-2020[symbol: see text] in a dose of 3.0 x 10(8) microbial cells/ml daily for 5-7 days prevented to the development of Vibrio cholerae infection. The curative effect observed after 3 administrations of lactobacilli within 48 hours after infection with V. cholerae was registered in 50% of cases. This strain of lactobacilli was found to be suitable for use as the basis component of probiotic, an additional remedy for the prophylaxis and treatment of cholera.

[Bactericidal properties of hemo-cytolysin from Vibrio cholerae non O1 P-11702 strain in a panel of indicator cultures for detection of vibriocins]. / [Bakteritsidnye svoistva gemotsitolizina iz shtamma Vibrio cholerae non O1 P-11702 na nabore indikatornykh kul'tur dlia vyiavleniia vibriotsinov.

The influence of the preparation of hemo-cytolysin, obtained from V. cholerae non O1 strain P-11702 and inducing lysis of both red blood cells and V. cholerae cultures using a panel of indicator cultures for the detection of vibriocins, was studied. The set of indicator cultures contained 2 Shigella flexneri strains, 1 S. dysenteriae strain, 3 S. sonnei strains, 3 Escherichia coli strains and 2 V. cholerae strains, one of them being atypical. Hemo-cytolysin exhibited lytic activity with respect to S. dysenteriae, S. sonnei strains and 1 V. cholerae strain. i.e. to 4 out of 11 indicator strains. V. cholerae atypical strain proved to be resistant to the preparation in contrast to 33 V. cholerae typical strains, studied previously.

[Bactericidal properties of the Vibrio cholerae non O1 bacteriolysin]. / Bakteritsidnye svoistva gemotsitolizina Vibrio cholerae non O1.

The influence of the preparation of hemocytolysin, obtained from V. cholerae non O1 (strain P-11702), on the growth of V. cholerae cells was studied. The study revealed that hemocytolysin is capable of inducing partial or complete bacterial lysis on the place of its application, depending on the protein load of the substance and the inoculation dose of microbes. Two electrophoretic fractions with molecular weights of 14 and 14.5 kD induced the cytolysis of sheep, rabbit, guinea-pig red blood cells and showed a bactericidal effect. The different sensitivity of Vct+ and Vct- strains of V. cholerae to different doses of hemocytolysin was studied.