ABSTRACT
The impact of gynecological operations on the rate of lipid peroxidation (LPO) and erythrocytic antioxidant defense (EAOD) was studied in women with uterine myoma. The higher rate of LPO and the decreased level of vitamin E were preoperatively found. A further increase in LPO activity and a reduction in vitamin E concentrations were observed 1-4 days after surgery. Recovery of the study parameters was seen on days 5-7 postoperatively. The enzyme glutathione-S-transferase was ascertained to play a pivotal role in the EAOD system.
Subject(s)
Antioxidants/metabolism , Hysterectomy , Laparoscopy , Leiomyoma/blood , Lipid Peroxidation , Uterine Neoplasms/blood , Uterus/surgery , Adult , Erythrocytes/enzymology , Erythrocytes/metabolism , Female , Glutathione Transferase/blood , Humans , Leiomyoma/pathology , Leiomyoma/surgery , Malondialdehyde/blood , Superoxide Dismutase/blood , Uterine Neoplasms/pathology , Uterine Neoplasms/surgery , Uterus/metabolism , Uterus/pathology , Vitamin E/bloodABSTRACT
Investigation of 213 rats has shown that exposition containing normal plasma of erythrocytes, neutrophils or monocytes (physiological concentration) taken from the rats treated with prooxidant (lead acetate) or antioxidant (selmevite) causes corresponding increase or decrease of: a) both spontaneous aggregation and ATP-induced aggregation of thrombocytes, b) releasing P3 and P4 factors, c) their lipoperoxidation (LPO) intensity. The effect is released by LPO products released by erythrocytes and leucocytes into their environment. The efficacy of antioxidants (vitamins with antioxidant properties in particular) used for correcting hypercoagulative changes in oxidative stress in clinics is supposed to be due to their capacity for limiting lipidoxidation in erythrocytes, leukocytes and thrombocytes.
Subject(s)
Erythrocytes/metabolism , Homeostasis/drug effects , Lipid Peroxidation/drug effects , Monocytes/metabolism , Neutrophils/metabolism , Adenosine Diphosphate/pharmacology , Animals , Antioxidants/pharmacology , Blood Platelets/metabolism , Organometallic Compounds/pharmacology , Organophosphorus Compounds/pharmacology , Platelet Aggregation/drug effects , RatsABSTRACT
Experiments on a group of 308 rats showed that prooxidant (lead acetate) introduced per os with daily meals decreases the antioxidant potential (increases lipid peroxidation, LPO) and reduces tolerance with respect to thrombin (increases the level of intravascular blood coagulation markers). For the same treatment on the background of a vitamin - antioxidants preparations (complivit or selmevit), the level of changes was significantly decreased and the resistance to thrombin was increased. Selmevit (containing selenium) was more effective. It is concluded that complex vitamin--antioxidants preparations can be used for correcting hemostatic changes in cases of hyperoxidation.
