ABSTRACT
Impact of choline alphoscerate on morphological hepatic indices in rats with hepatic cirrhosis and hepatic encephalopathy, caused by injection of 15% solution of CCl(4) during 4 weeks, in comparison to standard scheme of treatment, was studied. Reduction of the hepatic histological changes severity, of the injured hepatocytes volume, of stromalparenchymal ratio and enhancement of nucleocytoplasmic ratio in rats with hepatic cirrhosis under the choline alphoscerate impact were noted. The fibrosis and histological activity index in accordance to Knodell's scale witnessed a reduction of severity of hepatic affection in rats, which were treated, using choline alphoscerate.
Subject(s)
Glycerylphosphorylcholine/pharmacology , Hepatic Encephalopathy/drug therapy , Liver Cirrhosis/drug therapy , Liver/drug effects , Protective Agents/pharmacology , Animals , Carbon Tetrachloride , Cell Nucleus/drug effects , Cell Nucleus/ultrastructure , Cell Size , Cytoplasm/drug effects , Cytoplasm/ultrastructure , Dipeptides/pharmacology , Hepatic Encephalopathy/chemically induced , Hepatic Encephalopathy/pathology , Hepatocytes/drug effects , Hepatocytes/pathology , Hepatocytes/ultrastructure , Histocytochemistry , Lactulose/pharmacology , Liver/pathology , Liver/ultrastructure , Liver Cirrhosis/chemically induced , Liver Cirrhosis/pathology , Male , Rats , Rifamycins/pharmacology , RifaximinABSTRACT
Connection of the cytokins profile with experimental hepatic encephalopathy (HE) in rats was studied. Investigation was conducted on 20 laboratory rats, in which HE was simulated, using СCl4 injection. The Ñnterleukins (ÐL) level, including, ÐL1ß, ÐL4, ÐL10 and interferonγ(IFNγ), was determined using immunoassay method with the help of polyclonal antibodies. Enhancement of the proinflammatory ÐL1ß and ÐÐФγ content in the rats blood serum while induced HE by 57.9 and 39.5% accordingly (p <0.05), comparing with such in a control and the compensation enhancement of the anti'inflammatory IL4 and IL10 level by 34.6 and 75.9% (p < 0.05) was established. The results obtained confirms the cytokins role in pathogenesis of HE, determination of their level constitutes significant criterion for the posttransplantation complications prog' nostication. Inflammation and profile of cytokins constitute the main target in therapy of HE in patients, suffering liver cirrhosis
Subject(s)
Hepatic Encephalopathy/immunology , Interleukin-10/immunology , Interleukin-1beta/immunology , Interleukin-4/immunology , Animals , Carbon Tetrachloride , Disease Models, Animal , Enzyme-Linked Immunosorbent Assay , Gene Expression , Hepatic Encephalopathy/chemically induced , Hepatic Encephalopathy/genetics , Hepatic Encephalopathy/pathology , Humans , Inflammation , Interleukin-10/blood , Interleukin-10/genetics , Interleukin-1beta/blood , Interleukin-1beta/genetics , Interleukin-4/blood , Interleukin-4/genetics , RatsABSTRACT
It was studied the effect of probiotic strains of Bifidobacterium animalis VKL, Bifidobacterium animalis VKB and Lactobacillus casei IMVB-7280, and their mixtures on erosive and ulcerative lesions of the gastric mucosa (GM) of rats. GM was induced by water-immersion restraint stress. It was found that investigated probiotics did not have gastroprotective properties under a single and seven-day prophylactic administration. However, multiprobiotics (polyprobiotic Bifidobacterium animalis VKL and Bifidobacterium animalis VKB and composite probiotic Bifidobacterium animalis VKL, Bifidobacterium animalis VKB and Lactobacillus casei IMVB-7280) reduced the erosive and ulcerative lesions and the intensity of bleeding in rat GM when given within 14 days. It was shown that one of the mechanisms of antiulcer preventive effect of the multistrain probiotics is the restoration of pro/antioxidant balance in the GM under the stress action. The obtained results show the effectiveness of poly- and composite probiotics in the gastric ulcer prevention.
Subject(s)
Bifidobacterium/physiology , Gastrointestinal Hemorrhage/diet therapy , Immobilization/adverse effects , Lacticaseibacillus casei/physiology , Probiotics/pharmacology , Stomach Ulcer/diet therapy , Animals , Animals, Outbred Strains , Female , Gastric Mucosa/blood supply , Gastric Mucosa/drug effects , Gastric Mucosa/pathology , Gastrointestinal Hemorrhage/etiology , Gastrointestinal Hemorrhage/pathology , Rats , Stomach/blood supply , Stomach/drug effects , Stomach/pathology , Stomach Ulcer/etiology , Stomach Ulcer/pathology , Stress, PhysiologicalABSTRACT
The effect of a mixture of probiotic strains (2:1:1 Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, Bifidobacterium animalis VKB) on the development of experimental obesity in rats induced by neonatal administration of monosodium glutamate has been studied. It was shown that in rats of 4 months age, the injection of monosodium glutamate (4 mg/g) at 2, 4, 6, 8, 10 days after birth elicited abdominal obesity and metabolic syndrome. An intermittent administration of a probiotic mixture to rats treated with monosodium prevented the development of obesity. In the group of rats treated with probiotics, anthropometric parameters (weight and body length, Lee index, body mass index) did not differ from the level of intact rats. Visceral fat mass was decreased by probiotics by 38.5% (P < 0.05) compared to rats treated with water. Probiotics improved lipid metabolism: reduced the level of VLDL by 32.2% (P < 0,05), the level of LDL by 30.6% (P < 0.05), increased HDL by 25.7% (P <0,05) compared to obese control rats. Probiotic strains restored the secretion of adipocytes hormones (leptin and adiponectin) to the normal level of intact animals. The results show the effectiveness of probiotics for the prevention of obesity.
Subject(s)
Abdominal Fat/drug effects , Adipocytes/drug effects , Metabolic Syndrome/diet therapy , Obesity/diet therapy , Probiotics/administration & dosage , Sodium Glutamate/adverse effects , Abdominal Fat/metabolism , Abdominal Fat/pathology , Adipocytes/metabolism , Adipocytes/pathology , Adiponectin/biosynthesis , Adiponectin/metabolism , Alanine Transaminase/blood , Animals , Animals, Newborn , Aspartate Aminotransferases/blood , Bifidobacterium/physiology , Body Mass Index , Body Size , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Cholesterol, VLDL/blood , Lacticaseibacillus casei/physiology , Leptin/biosynthesis , Leptin/metabolism , Lipid Metabolism , Male , Metabolic Syndrome/chemically induced , Metabolic Syndrome/metabolism , Metabolic Syndrome/pathology , Obesity/chemically induced , Obesity/metabolism , Obesity/pathology , RatsABSTRACT
It was investigated the effect of probiotic strains Bifidobacterium animalis VKL and VKB and their mixture on erosive and ulcerative lesions in the gastric mucosa (GM) of rats induced by water immersion restraint stress. It was found that separate prophylactic introduction for 14 days of Bifidobacterium animalis VKL or Bifidobacterium animalis VKB didn't protect the GM from erosive and ulcerative lesions induced by stress. Contrary prophylactic introduction of Bifidobacterium animalis VKL and VKB mixture has been effective in protecting the GM from the lesions. One of the mechanisms of the gastroprotection of these probiotic strains is prevention of mucus barrier from degradation, which was evident in decrease of free fucose and hexuronic acids content. These results confirm the expediency ofprobiotics use for the prevention of stress-induced lesions in the GM.
