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1.
Nucleic Acids Res ; 42(15): 9677-90, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25081210

ABSTRACT

The bacterial transcription terminator, Rho, terminates transcription at half of the operons. According to the classical model derived from in vitro assays on a few terminators, Rho is recruited to the transcription elongation complex (EC) by recognizing specific sites (rut) on the nascent RNA. Here, we explored the mode of in vivo recruitment process of Rho. We show that sequence specific recognition of the rut site, in majority of the Rho-dependent terminators, can be compromised to a great extent without seriously affecting the genome-wide termination function as well as the viability of Escherichia coli. These terminators function optimally only through a NusG-assisted recruitment and activation of Rho. Our data also indicate that at these terminators, Rho-EC-bound NusG interaction facilitates the isomerization of Rho into a translocase-competent form by stabilizing the interactions of mRNA with the secondary RNA binding site, thereby overcoming the defects of the primary RNA binding functions.


Subject(s)
RNA-Binding Proteins/metabolism , Rho Factor/metabolism , Transcription Termination, Genetic , Adenosine Triphosphate/metabolism , Binding Sites , Escherichia coli Proteins/genetics , Mutation , Peptide Elongation Factors/genetics , Prophages/genetics , RNA, Messenger/chemistry , RNA, Messenger/metabolism , RNA-Binding Proteins/genetics , Rho Factor/genetics , Transcription Factors/genetics , Transcriptome
2.
EcoSal Plus ; 6(1)2014 May.
Article in English | MEDLINE | ID: mdl-26442934

ABSTRACT

The highly conserved Nus factors of bacteria were discovered as essential host proteins for the growth of temperate phage λ in Escherichia coli. Later, their essentiality and functions in transcription, translation, and, more recently, in DNA repair have been elucidated. Close involvement of these factors in various gene networks and circuits is also emerging from recent genomic studies. We have described a detailed overview of their biochemistry, structures, and various cellular functions, as well as their interactions with other macromolecules. Towards the end, we have envisaged different uncharted areas of studies with these factors, including their participation in pathogenicity.

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