ABSTRACT
Methods for evaluating the potency of inactivated rabies vaccines are reviewed. Shortcomings of the traditional NIH method and advantages of modern rapid immunological in vitro methods (antibody binding test, radial immunodiffusion test, enzyme linked immunoadsorbent assay) for estimation of antigenic activity of vaccines are discussed.
Subject(s)
Rabies Vaccines/immunology , Vaccines, Inactivated/immunology , Binding Sites, Antibody , Enzyme-Linked Immunosorbent Assay , ImmunodiffusionABSTRACT
Subpopulation composition of 8 ASFV isolates and variants differing in virulence was evaluated comparatively by their haemadsorption capacity. The "quantitative haemadsorption marker" was shown to be useful for characterization of the strains, virus population phenotypic heterogeneity and structure. The marker expression was found to correlate with virulence: attenuated variants had low haemadsorption and more subpopulation components with that shift, and vice versa.