ABSTRACT
A method using high-performance anion-exchange chromatography (HPAEC) with a pulsed amperometric detector (PAD) for the determination of mono- and disaccharides is described. The method was accepted by the International Dairy Federation and the Internal Organization for Standardization as a new work item for the determination of sugars in dairy matrixes, and the Milk and Milk Products technical committee of ISO/TC 34/SC 5 accepted the topic "Milk and milk products - Determination of the sugar contents - High-performance anion-exchange chromatographic method (HPAEC-PAD)" as a new work item. The proposed method consists of an aqueous ethanol extraction of the sugars in the dairy sample, followed by clarification with Carrez I and II reagents. The clarified filtrate is diluted and then directly introduced in the HPAEC-PAD system for quantification of the sugars. A single-laboratory validation of the proposed method has been scheduled for spring 2017.
Subject(s)
Carbohydrates/analysis , Chromatography, High Pressure Liquid/standards , Chromatography, Ion Exchange/standards , Dairy Products/analysis , Milk/chemistry , AnimalsABSTRACT
In a paper presented at the Institute of Hospital Engineering Australia's (IHEA) 59th Annual National Conference in Canberra last year, Klaas Visser, of KAV Consulting, examines effective ways to reduce CO2 emissions and water use in hospitals.