ABSTRACT
BACKGROUND: Oral leukoplakia is the most common precancerous lesion. Various grading systems based on histological findings have been proposed for dysplasia. Recently, computer-assisted morphometric analysis has been established to reduce the interobserver and intraobserver variability during the histological grading of epithelial dysplasia. This study was undertaken to establish the morphometric changes in the suprabasal cell layer of different grades of oral epithelial dysplasia. MATERIALS AND METHODS: Forty paraffin-embedded tissue sections (10 normal mucosa, 10 mild dysplasia, 10 moderate dysplasia, and 10 severe dysplasia cases) were stained using hematoxylin and eosin stain, and analyzed for cellular and nuclear morphometry using binocular microscope. RESULTS: Our results showed that values of nuclear area, nuclear perimeter, nuclear volume density, and nuclear/cytoplasmic (N/C) ratio were increased gradually in dysplasia compared to control groups and the values were statistically significant (P = 0.001). Nuclear diameter and cellular area were increased in dysplasia when compared to control group (P = 0.001). Mild and moderate dysplasia showed decreased value of nuclear form factor compared to control group, whereas severe dysplasia showed highest value. A fair correlation was found when comparing histological grading and grouping based on nuclear area, nuclear perimeter, N/C ratio, and nuclear volume density. CONCLUSION: Nuclear features reflect cell behavior, and its morphometric analysis can be considered as a reliable tool for differentiating various grades of epithelial dysplasia.
ABSTRACT
INTRODUCTION: Endodontically treated teeth have significantly differentphysical properties compared to vital teeth. To ensure functional longevity,endodontically treated teeth must have at least 5 mm of tooth structure coronalto the crestal bone, 2 mm of coronal tooth structure incisal to the preparationfinish line are necessary to ensure functional integrity. AIM AND OBJECTIVES: To evaluate fracture resistance of endodontically treated teeth restored withcustom cast post, custom cast post with variable ferrule height and teethwithout ferrule, prefabricated post with variable ferrule height and teethwithout ferrule. To compare fracture resistance of teeth restored with customcast post and prefabricated post with variable ferrule heights. MATERIALS AND METHODS: Methodology includes selection of teeth, root canal preparation,post space preparation, grouping of samples, Group A-custom cast post andcore with sub groups of variable ferrule height, pattern fabrication, Group A- pattern fabrication for customized cast post. Group B-pattern fabricationfor prefabricated post and core, investing and casting, cementation, custommade acrylic jig preparation, testing of specimens. RESULTS: The differencein the fracture load between the samples of Group A was highly significantat the 0.001 level. Subgroup A4 had highest fracture resistance while GroupA, possessed the least fracture resistance. Subgroups A2, A3 had fractureresistance value intermediate between A1, A4. CONCLUSION: Increasing theferrule height significantly increases (P < 0.001) the fracture resistance ofendodontically treated teeth restored with both custom made cast post andcore and prefabricated post with metal core. Comparatively the custom madecast post and core with variable ferrule height, especially 2 mm ferrule showedsignificant fracture resistance than prefabricated post with metal core. Thepresence of 2 mm ferrule height significantly increases (P < 0.001).
