Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 4 de 4
Filter
Add more filters










Database
Language
Publication year range
1.
Vopr Virusol ; 60(6): 41-5, 2015.
Article in Russian | MEDLINE | ID: mdl-27024917

ABSTRACT

The goal of this work was to demonstrate the results of the development of the enzyme-linked immunosorbent tests with chemiluminescence detection and colorimetric detection of specific viral antigens and antibodies for identifying the avian influenza and the Newcastle disease viruses: high sensitivity and specificity of the immuno- chemiluminescence assay, which are 10-50 times higher than those of the ELISA colorimetric method. The high effectiveness of the results and the automation of the process of laboratory testing (using a luminometer) allow these methods to be recommended for including in primary screening tests for these infectious diseases.


Subject(s)
Antibodies, Viral/blood , Antigens, Viral/blood , Biological Assay , Influenza in Birds/diagnosis , Newcastle Disease/diagnosis , Poultry Diseases/diagnosis , Animals , Chickens , Colorimetry , Enzyme-Linked Immunosorbent Assay , Hemagglutination Inhibition Tests , Influenza A virus/immunology , Influenza in Birds/blood , Influenza in Birds/immunology , Influenza in Birds/virology , Luminescence , Newcastle Disease/blood , Newcastle Disease/immunology , Newcastle Disease/virology , Newcastle disease virus/immunology , Poultry Diseases/blood , Poultry Diseases/immunology , Poultry Diseases/virology , Sensitivity and Specificity
2.
Vopr Virusol ; 53(5): 4-8, 2008.
Article in Russian | MEDLINE | ID: mdl-19069785

ABSTRACT

The epizootic etiologically associated with highly pathogenic avian influenza H5N1 genotype 2.3.2 that is new for Russia among wild and domestic birds in the south of the Primorye Territory during spring migration in April 2008 has been decoded. About 25% of the wild birds of a water complex, which include European teals (Anas crecca), mallard ducks (Anas platyrhynchos), great-crested grebes (Podiceps cristatus), are involved in viral circulation in the area of the Suifun-Khankai plain. Chicken embryos and the cell lines MDCK, SPEV, BHK-21, SW-13 were used to isolate 3 strains from recently deceased hens (A/chicken/Primorje/1/08, A/chicken/Primorje/11/08, and A/chicken/Primorje/12/08) and one strain from a European teal (A/Anas crecca/Primorje/8/08). The strains were deposited in the State Collection of Viruses of the Russian Federation, D. I. Ivanovsky Research Institute of Virology, Russian Academy of Medical Sciences. The nucleotide sequences of the full-sized genomes of A/chicken/Primorje/1/08 and A/Anas crecca/Primorje/8/08 were sent to the International databank GenBank. The strains from domestic and wild birds were shown to be identical. The isolated strains are most close to the strains Alchicken/Viet Nam/10/05, A/chicken/Guangdong/178/04, and A/duck/Viet Nam/12/05. Molecular genetic analysis has indicated that the strains isolated are susceptible to rimantadine and ozeltamivir and less adapted to mammalian cells (particularly, they contain E627 in RV2, which agrees with the biological properties of these strains in vitro). Penetration of the newly isolated virus into the Far East ecosystem provides in the foreseeable future a way for infecting the birds wintering in America and Australia in the nesting places, with further carriage of viral populations there in the period of autumn migrations.


Subject(s)
Disease Outbreaks , Influenza A Virus, H5N1 Subtype/classification , Influenza A Virus, H5N1 Subtype/genetics , Influenza in Birds/epidemiology , Influenza in Birds/virology , Animal Migration , Animals , Antiviral Agents/pharmacology , Birds/virology , Chickens/virology , Genome, Viral , Genotype , Influenza A Virus, H5N1 Subtype/drug effects , Influenza A Virus, H5N1 Subtype/isolation & purification , Molecular Sequence Data , Oseltamivir/pharmacology , Phylogeny , Rimantadine/pharmacology , Siberia/epidemiology
3.
Vopr Virusol ; 53(2): 14-9, 2008.
Article in Russian | MEDLINE | ID: mdl-18450104

ABSTRACT

Isolation, followed by the sequencing the full-size genome of strains of A/chicken/Krasnodarl300/07 and A/Cygnus cygnus/Krasnodar/329/07, has shown that they belong to genotype 2.2 (Qinghai-Siberian). The strains were deposited at the State Virus Collection of the Russian Federation and nucleotide consequences were at the International databank GenBank. The strains contained 10 unique amino acid replacements in reference to the consensus of the Qinghai-Siberian genotype in the PB2, PA, HA, NA, and NS1, which suggests that regional variants may form in different parts of an area.


Subject(s)
Animals, Wild/virology , Birds/virology , Disease Outbreaks/veterinary , Influenza A Virus, H5N1 Subtype/classification , Influenza in Birds/epidemiology , Influenza in Birds/virology , Amino Acid Substitution , Animals , Cell Line , Chick Embryo , Dogs , Genetic Variation , Genome, Viral , Influenza A Virus, H5N1 Subtype/genetics , Influenza A Virus, H5N1 Subtype/isolation & purification , Molecular Sequence Data , Phylogeny , Poultry/virology , Russia/epidemiology , Swine , Viral Proteins/genetics , Zoonoses
4.
Vopr Virusol ; 52(6): 40-7, 2007.
Article in Russian | MEDLINE | ID: mdl-18050717

ABSTRACT

Among agricultural birds in the near-Moscow Region (February 2007), local epizootics caused by the highly pathogenic avian influenza A/H5N1 virus seem to be of unintended manual origin. Such a situation may be considered to be model when the source of inoculation is elucidated in cases of potentially possible acts of bioterrorism. Molecular genetic analysis of isolated A/chicken/Moscow/2/2007 strain established its genetic similarity with the highly pathogenic strains detected in the Black-and-Caspian Sea region in 2006. At the same time, comparison of nucleotide sequences of the strain A/chicken/Moscow/2/2007 with the strains of Qinghai-Siberian genotype (CSG) for which the sequences of full-sized genomes are known in the international databases revealed a significant distinction of the near-Moscow strain from the earlier known analogues. The uniqueness of the primary structure of the PB1 gene is shown. The paper discusses the functional value of amino acid substitutions in the proteins of the strain A/chicken/Moscow/2/2007 and in other variants of CSG of the subtype H5N1.


Subject(s)
Disease Outbreaks , Influenza A Virus, H5N1 Subtype/genetics , Influenza in Birds/epidemiology , Influenza in Birds/virology , Zoonoses/virology , Amino Acid Substitution , Animals , Chick Embryo , Cricetinae , Dogs , Influenza A Virus, H5N1 Subtype/classification , Influenza A Virus, H5N1 Subtype/isolation & purification , Phylogeny , Poultry/virology , Russia/epidemiology , Sequence Homology , Viral Proteins/genetics
SELECTION OF CITATIONS
SEARCH DETAIL
...