ABSTRACT
In vitro experiments were shown that native ecologically pure non-starch polysaccharide crylan bind the bile acid. Crylan was found to decrease hyperlipidemia induced in rats by means of diet enriched with cholesterol and 6-methylthiouracil. This effect of crylan was more pronounced as compare with the action of native nonspecific enterosorbent polyfepan.
Subject(s)
Hyperlipidemias/drug therapy , Hypolipidemic Agents/therapeutic use , Polysaccharides/therapeutic use , Animals , Antithyroid Agents/administration & dosage , Cholesterol, Dietary/administration & dosage , Diet, Atherogenic , Enterosorption , Hyperlipidemias/etiology , Lignin/administration & dosage , Lipids/analysis , Lipids/blood , Liver/chemistry , Male , Methylthiouracil/administration & dosage , RatsABSTRACT
Mannan sulphates (MS) synthesized on the basis of extracellular linear mannan (LM) of Rhodotorula rubra induce resistance of Immune-580 tobacco and thornapple (Datura stramonium) to tobacco mosaic virus (TMV). The resistance is manifested in a decrease in the number and/or size of viral local lesions (LL) in MS-treated (mg/ml) leaf halves. The reduction in the LL size does not seem to be due to the direct inhibition of TMV multiplication by the polysaccharide as the virus accumulation in the tissues of the systemic host (Samsun tobacco) does not decrease when MS is used 15 min after infection. The induced resistance is partially inhibited by actinomycin D and completely by heating (32 degrees C). Unlike MS, neutral LM decreases only the number of LL on the treated parts of the tissue but does not influence their size. The antiviral activity of LM does not change in the presence of actinomycin D. The possible mechanisms of the LM and MS protective effect in plants are discussed.
Subject(s)
Mannans/therapeutic use , Plant Diseases , Dactinomycin/pharmacology , Drug Evaluation, Preclinical , Immunity, Innate/drug effects , Temperature , Time Factors , Tobacco Mosaic VirusABSTRACT
The effect of various carbon and nitrogen sources on the synthesis of the branched exopolysaccharide produced by Cryptococcus laurentii st 1803-K. was studied. The conditions favourable for a higher yield of the polymer were found. The carbon and nitrogen sources used do not affect the qualitative monosaccharide composition of the polysaccharide, however, the introduction of ammonium salts in the medium can cause a change in the monosaccharide ratio in the side chains of the polymer.
