ABSTRACT
The levels of adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), lactic dehydrogenase (LDH), and LDH isoenzyme patterns (LD1 to LD5) have been measured in lymphocyte extract from 28 patients with B-chronic lymphocytic leukemia (B-CLL). The activities of ADA, PNP, and LDH have been correlated with two morphological groups of B-CLL classified according to the percentage of large, nongranular, atypical lymphocytes (AL) in peripheral blood: "typical" B-CLL (less than 10% of AL, 21 cases) and "atypical" B-CLL (10-25% of AL, seven cases). Patients with atypical B-CLL had significantly (P less than 0.001) higher activities of ADA (0.46 +/- 0.17 U/10(9) cells), PNP (1.74 +/- 1.0 U/10(9) cells), and LDH (48.3 +/- 9.7 U/10(9) cells) than patients with typical B-CLL (ADA, 0.29 +/- 0.1 U/10(9) cells; PNP, 0.58 +/- 0.23 U/10(9) cells; and LDH, 29 +/- 10 U/10(9) cells). In addition, the "treatment-free period" was also significantly (P less than 0.025) shorter in the group of atypical B-CLL compared with the typical B-CLL group. No clear-cut statistical differences in lymphocyte surface markers or in several other prognostic factors between the two subgroups of B-CLL were found. The present study supports the idea that in B-CLL the simultaneous determination of ADA, PNP, and LDH might be helpful in better understanding the pathophysiology, prognosis, and natural history of the disease.
Subject(s)
Adenosine Deaminase/analysis , L-Lactate Dehydrogenase/analysis , Leukemia, Lymphoid/enzymology , Nucleoside Deaminases/analysis , Pentosyltransferases/analysis , Purine-Nucleoside Phosphorylase/analysis , Antigens, Differentiation/analysis , B-Lymphocytes , Biopsy , Humans , Leukemia, Lymphoid/pathology , Lymphocytes/immunology , PrognosisSubject(s)
Anemia, Aplastic/therapy , Bone Marrow Transplantation , Adolescent , Adult , Blood Transfusion , Child , Female , Graft Rejection , Graft vs Host Reaction , Humans , MaleABSTRACT
Abnormally high levels of IgE are produced in various diseases, including atopic eczema, and a functional decrease in the T-lymphocyte population occurs at the same time. T-cell function has been studied in a group of asthma patients with high, normal and low IgE levels and in a control group. T-cell function appears to be reduced in asthmatic patients with high concentrations of IgE when skin tests are performed with Candida antigen (P less than 0.05). There was also a decline in the lymphocyte transformation tests with candida antigen in autologous serum (P less than 0.05), although not in foetal calf serum. The responses to in vivo tests with streptokinase-streptodornase were normal, as was the response of the lymphocytes to PHA.
