[The design of a diagnostic test system based on magnetic sorbents and liposomes]. / Konstruirovanie diagnosticheskoi test-sistemy na osnove magnosorbentov i liposom.

The results obtained in the study of the possibility of using magnetic sorbents for the construction of a diagnostic assay system based on the antigen-antibody interaction are presented. As a model, Yersinia pestis capsular antigen and immunoglobulins to it have been used. A solid-phase immunofluorescent liposomal assay method has been developed; this method can be used for the detection of biopolymers in the sample under study and for the determination of their activity.

[Immunofluorescence method of detecting cholera enterotoxin]. / Immunofliuorestsentnyi metod obnaruzheniia kholernogo énterotoksina.

The quantitative immunofluorescent assay for the determination of cholera enterotoxin is proposed. The assay is based on the selective sorption of cholera enterotoxin by gangliosides incorporated into polyacrylamide granules. The preliminary treatment of gangliosides with neuraminidase enhances the sensitivity of this assay. The assay permits the detection of cholerigen in an amount of 20 ng.

[Use of neuraminidase for improving the properties of the erythrocytic ganglioside diagnosticum]. / Ispol'zovanie neiraminidazy dlia uluchsheniia svoistv eritrotsitarnogo gangliozidnogo diagnostikuma.

In this work the possibility of using neuraminidase for increasing the content of ganglioside GM1 in the mixture of gangliosides used for the sensitization of erythrocytes has been studied. The study has revealed that the treatment of gangliosides with neuraminidase is sufficient for obtaining active hemosensitin; there is no need for the purification of the preparation by gel filtration.

[Production of an erythrocytic ganglioside diagnostic reagent for detecting cholera enterotoxin and toxoid in indirect hemagglutination tests]. / Poluchenie éritrotsitarnogo gangliozidnogo diagnostikuma dlia vyiavleniia kholernogo énterotoksina i toksoida v reaktsii nepriamoi gemagglutinatsii.

The studies described in this work have indicated that cholera enterotoxin and its components (cholerogenoid and subunit B) can be detected in amounts of 0.25, 0.28 and 0.6 microgram of protein per 1 ml, respectively, by means of erythrocytes sensitized with gangliozide-containing complex. The conditions for erythrocyte sensitization have been established. The methods of cholerogen titration in the passive hemagglutination test by means of the erythrocytic gangliozide diagnostic reagent and in Craig's skin test have been shown to correlate. Similarly, the passive hemagglutination test is supposed to be suitable for detecting other bacterial toxins interacting with gangliozides.

[Method of extracting cholera toxin from solid media containing cultured vibrios]. / Sposob izvlecheniiia kholernogo toksina iz plotnykh sred pri vyrashchivanii na nikh vibrionov.

The possibility of obtaining biologically active cholerogen by extracting it from solid culture media used for the cultivation of vibrios is shown. V. cholerae, strain 569B, cultivated on solid media produced about 3 times more toxin per 1 ml of the culture medium than during the process of its cultivation with the use of liquid culture media. The inculation temperature proved to have no essential influence on the toxin production by vibrios.