ABSTRACT
Sera of 30 patients with disseminated processes in the lungs of various etiology were examined for the presence of specific IgG to antigens of Aspergillus fumigatus (A.f.) and Candida albicans (C.a.) using solid phase enzyme immunoassay. Elevated concentrations of IgG to A. f. and C. a. were found in 6 and 12 patients, respectively. Serodiagnosis was confirmed by a direct detection of the fungi in the bronchi (cytology of the lavage, culturing). It is highly probable that opportunistic fungi may be involved in pulmonary dissemination. Therefore, patients with disseminated pulmonary diseases should be examined for opportunistic fungi.
Subject(s)
Aspergillosis/diagnosis , Aspergillus fumigatus , Lung Diseases, Fungal/diagnosis , Opportunistic Infections/diagnosis , Adolescent , Adult , Aged , Antibodies, Fungal/blood , Antibody Specificity , Aspergillosis/etiology , Aspergillus fumigatus/immunology , Candida albicans/immunology , Female , Humans , Immunoglobulins/blood , Lung/diagnostic imaging , Lung Diseases, Fungal/etiology , Male , Middle Aged , Opportunistic Infections/etiology , RadiographyABSTRACT
Enzyme-linked immunoassay (ELISA) was employed for determination of specific immunoglobulin G to Aspergillus fumigatus (A.f.). Culture filtrate of A.f. was used as antigen. Optimal conditions of ELISA were found. Serum of 138 asthmatic patients with total IgE level more than 500 PRU/ml were analysed for determining the limits of IgG to A.f. concentrations.
Subject(s)
Antibodies, Fungal/analysis , Aspergillosis/diagnosis , Aspergillus fumigatus/immunology , Enzyme-Linked Immunosorbent Assay , Immunoglobulin G/analysis , Lung Diseases, Fungal/diagnosis , Adolescent , Adult , Aged , Aspergillosis/immunology , Asthma/immunology , Female , Humans , Lung Diseases, Fungal/immunology , Male , Middle AgedABSTRACT
Fifteen bronchial asthma patients were followed-up for 6 months. They were administered beclomet-250 in a daily dose 1000 micrograms. The patients' clinical status was characterized by positive changes, ventilation function of the lungs improved. The drug was discovered to produce no suppressive action on adrenal function. Besides, the patients did not develop oropharyngeal candidiasis.