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1.
Methods Mol Biol ; 2431: 429-449, 2022.
Article in English | MEDLINE | ID: mdl-35412291

ABSTRACT

The use of primary neuronal cultures generated from Drosophila tissue provides a powerful model for studies of transport mechanisms. Cultured fly neurons provide similarly detailed subcellular resolution and applicability of pharmacology or fluorescent dyes as mammalian primary neurons. As an experimental advantage for the mechanistic dissection of transport, fly primary neurons can be combined with the fast and highly efficient combinatorial genetics of Drosophila, and genetic tools for the manipulation of virtually every fly gene are readily available. This strategy can be performed in parallel to in vivo transport studies to address relevance of any findings. Here we will describe the generation of primary neuronal cultures from Drosophila embryos and larvae, the use of external fluorescent dyes and genetic tools to label cargo, and the key strategies for live imaging and subsequent analysis.


Subject(s)
Axonal Transport , Drosophila , Animals , Axonal Transport/physiology , Axons/metabolism , Drosophila/genetics , Fluorescent Dyes/metabolism , Kinesins , Mammals , Neurons
2.
PLoS Genet ; 17(7): e1009647, 2021 07.
Article in English | MEDLINE | ID: mdl-34228717

ABSTRACT

The formation and maintenance of microtubules requires their polymerisation, but little is known about how this polymerisation is regulated in cells. Focussing on the essential microtubule bundles in axons of Drosophila and Xenopus neurons, we show that the plus-end scaffold Eb1, the polymerase XMAP215/Msps and the lattice-binder Tau co-operate interdependently to promote microtubule polymerisation and bundle organisation during axon development and maintenance. Eb1 and XMAP215/Msps promote each other's localisation at polymerising microtubule plus-ends. Tau outcompetes Eb1-binding along microtubule lattices, thus preventing depletion of Eb1 tip pools. The three factors genetically interact and show shared mutant phenotypes: reductions in axon growth, comet sizes, comet numbers and comet velocities, as well as prominent deterioration of parallel microtubule bundles into disorganised curled conformations. This microtubule curling is caused by Eb1 plus-end depletion which impairs spectraplakin-mediated guidance of extending microtubules into parallel bundles. Our demonstration that Eb1, XMAP215/Msps and Tau co-operate during the regulation of microtubule polymerisation and bundle organisation, offers new conceptual explanations for developmental and degenerative axon pathologies.


Subject(s)
Axons/metabolism , Microtubule-Associated Proteins/metabolism , Microtubules/metabolism , Animals , Axons/physiology , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Microtubule-Associated Proteins/physiology , Microtubules/physiology , Neurons/metabolism , Polymerization , Xenopus Proteins/metabolism , Xenopus laevis/metabolism , tau Proteins/metabolism
3.
Acta Neuropathol Commun ; 8(1): 158, 2020 09 07.
Article in English | MEDLINE | ID: mdl-32894207

ABSTRACT

A large intronic hexanucleotide repeat expansion (GGGGCC) within the C9orf72 (C9orf72-SMCR8 Complex Subunit) locus is the most prevalent genetic cause of both Frontotemporal Dementia (FTD) and Motor Neuron Disease (MND). In patients this expansion is typically hundreds to thousands of repeat units in length. Repeat associated non-AUG translation of the expansion leads to the formation of toxic, pathological Dipeptide-Repeat Proteins (DPRs). To date there remains a lack of in vivo models expressing C9orf72 related DPRs with a repeat length of more than a few hundred repeats. As such our understanding of how physiologically relevant repeat length DPRs effect the nervous system in an ageing in vivo system remains limited. In this study we generated Drosophila models expressing DPRs over 1000 repeat units in length, a known pathological length in humans. Using these models, we demonstrate each DPR exhibits a unique, age-dependent, phenotypic and pathological profile. Furthermore, we show co-expression of specific DPR combinations leads to distinct, age-dependent, phenotypes not observed through expression of single DPRs. We propose these models represent a unique, in vivo, tool for dissecting the molecular mechanisms implicated in disease pathology, opening up new avenues in the study of both MND and FTD.


Subject(s)
DNA Repeat Expansion/genetics , Dipeptides/genetics , Disease Models, Animal , Frontotemporal Dementia , Motor Neuron Disease , Animals , C9orf72 Protein/genetics , Drosophila , Phenotype
4.
Elife ; 82019 11 13.
Article in English | MEDLINE | ID: mdl-31718774

ABSTRACT

Cortical collapse factors affect microtubule (MT) dynamics at the plasma membrane. They play important roles in neurons, as suggested by inhibition of axon growth and regeneration through the ARF activator Efa6 in C. elegans, and by neurodevelopmental disorders linked to the mammalian kinesin Kif21A. How cortical collapse factors influence axon growth is little understood. Here we studied them, focussing on the function of Drosophila Efa6 in experimentally and genetically amenable fly neurons. First, we show that Drosophila Efa6 can inhibit MTs directly without interacting molecules via an N-terminal 18 amino acid motif (MT elimination domain/MTED) that binds tubulin and inhibits microtubule growth in vitro and cells. If N-terminal MTED-containing fragments are in the cytoplasm they abolish entire microtubule networks of mouse fibroblasts and whole axons of fly neurons. Full-length Efa6 is membrane-attached, hence primarily blocks MTs in the periphery of fibroblasts, and explorative MTs that have left axonal bundles in neurons. Accordingly, loss of Efa6 causes an increase of explorative MTs: in growth cones they enhance axon growth, in axon shafts they cause excessive branching, as well as atrophy through perturbations of MT bundles. Efa6 over-expression causes the opposite phenotypes. Taken together, our work conceptually links molecular and sub-cellular functions of cortical collapse factors to axon growth regulation and reveals new roles in axon branching and in the prevention of axonal atrophy. Furthermore, the MTED delivers a promising tool that can be used to inhibit MTs in a compartmentalised fashion when fusing it to specifically localising protein domains.


Subject(s)
Axons/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Guanine Nucleotide Exchange Factors/metabolism , Membrane Proteins/metabolism , Microtubules/metabolism , Polymerization , Amino Acid Motifs , Animals , Cell Membrane/metabolism , Cells, Cultured , Drosophila Proteins/chemistry , Fibroblasts/metabolism , Green Fluorescent Proteins/metabolism , Growth Cones/metabolism , Guanine Nucleotide Exchange Factors/chemistry , Membrane Proteins/chemistry , Mice , NIH 3T3 Cells , Peptides/metabolism , Protein Domains , Pseudopodia/metabolism
5.
Neural Dev ; 14(1): 11, 2019 11 09.
Article in English | MEDLINE | ID: mdl-31706327

ABSTRACT

Axons are the slender, cable-like, up to meter-long projections of neurons that electrically wire our brains and bodies. In spite of their challenging morphology, they usually need to be maintained for an organism's lifetime. This makes them key lesion sites in pathological processes of ageing, injury and neurodegeneration. The morphology and physiology of axons crucially depends on the parallel bundles of microtubules (MTs), running all along to serve as their structural backbones and highways for life-sustaining cargo transport and organelle dynamics. Understanding how these bundles are formed and then maintained will provide important explanations for axon biology and pathology. Currently, much is known about MTs and the proteins that bind and regulate them, but very little about how these factors functionally integrate to regulate axon biology. As an attempt to bridge between molecular mechanisms and their cellular relevance, we explain here the model of local axon homeostasis, based on our own experiments in Drosophila and published data primarily from vertebrates/mammals as well as C. elegans. The model proposes that (1) the physical forces imposed by motor protein-driven transport and dynamics in the confined axonal space, are a life-sustaining necessity, but pose a strong bias for MT bundles to become disorganised. (2) To counterbalance this risk, MT-binding and -regulating proteins of different classes work together to maintain and protect MT bundles as necessary transport highways. Loss of balance between these two fundamental processes can explain the development of axonopathies, in particular those linking to MT-regulating proteins, motors and transport defects. With this perspective in mind, we hope that more researchers incorporate MTs into their work, thus enhancing our chances of deciphering the complex regulatory networks that underpin axon biology and pathology.


Subject(s)
Axons/pathology , Axons/physiology , Homeostasis/physiology , Microtubules/physiology , Animals
6.
Cell Rep ; 22(4): 967-978, 2018 01 23.
Article in English | MEDLINE | ID: mdl-29386138

ABSTRACT

Maintenance of metabolic homeostasis requires adaption of gene regulation to the cellular energy state via transcriptional regulators. Here, we identify a role of ceramide synthase (CerS) Schlank, a multiple transmembrane protein containing a catalytic lag1p motif and a homeodomain, which is poorly studied in CerSs, as a transcriptional regulator. ChIP experiments show that it binds promoter regions of lipases lipase3 and magro via its homeodomain. Mutation of nuclear localization site 2 (NLS2) within the homeodomain leads to loss of DNA binding and deregulated gene expression, and NLS2 mutants can no longer adjust the transcriptional response to changing lipid levels. This mechanism is conserved in mammalian CerS2 and emphasizes the importance of the CerS protein rather than ceramide synthesis. This study demonstrates a double role of CerS Schlank as an enzyme and a transcriptional regulator, sensing lipid levels and transducing the information to the level of gene expression.


Subject(s)
Ceramides/metabolism , Drosophila Proteins/genetics , Drosophila melanogaster/genetics , Gene Expression Regulation/genetics , Sphingosine N-Acyltransferase/genetics , Animals
7.
Dev Biol ; 430(1): 52-68, 2017 10 01.
Article in English | MEDLINE | ID: mdl-28821389

ABSTRACT

Scavenger receptors class B (SR-B) are multifunctional transmembrane proteins, which in vertebrates participate in lipid transport, pathogen clearance, lysosomal delivery and intracellular sorting. Drosophila has 14 SR-B members whose functions are still largely unknown. Here, we reveal a novel role for the SR-B family member Debris buster (Dsb) in Drosophila airway physiology. Larvae lacking dsb show yeast avoidance behavior, hypoxia, and severe growth defects associated with impaired elongation and integrity along the airways. Furthermore, in dsb mutant embryos, the barrier function of the posterior spiracles, which are critical for gas exchange, is not properly established and liquid clearance is locally impaired at the spiracular lumen. We found that Dsb is specifically expressed in a group of distal epithelial cells of the posterior spiracle organ and not throughout the entire airways. Furthermore, tissue-specific knockdown and rescue experiments demonstrate that Dsb function in the airways is only required in the posterior spiracles. Dsb localizes in intracellular vesicles, and a subset of these associate with lysosomes. However, we found that depletion of proteins involved in vesicular transport to the apical membrane, but not in lysosomal function, causes dsb-like airway elongation defects. We propose a model in which Dsb sorts components of the apical extracellular matrix which are essential for airway physiology. Since SR-B LIMP2-deficient mice show reduced expression of several apical plasma membrane proteins, sorting of proteins to the apical membrane is likely an evolutionary conserved function of Dsb and LIMP2. Our data provide insights into a spatially confined function of the SR-B Dsb in intracellular trafficking critical for the physiology of the whole tubular airway network.


Subject(s)
Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Receptors, Scavenger/metabolism , Respiratory Physiological Phenomena , Respiratory System/metabolism , Animals , Drosophila Proteins/genetics , Drosophila melanogaster/cytology , Drosophila melanogaster/embryology , Embryo, Nonmammalian/metabolism , Extracellular Matrix/metabolism , Gene Expression Regulation , Hypoxia/metabolism , Intracellular Space/metabolism , Larva/metabolism , Mutation/genetics , Protein Transport , RNA Interference , Receptors, Scavenger/genetics , Water
8.
Semin Cell Dev Biol ; 69: 40-57, 2017 09.
Article in English | MEDLINE | ID: mdl-28579450

ABSTRACT

Spectraplakins are evolutionarily well conserved cytoskeletal linker molecules that are true members of three protein families: plakins, spectrins and Gas2-like proteins. Spectraplakin genes encode at least 7 characteristic functional domains which are combined in a modular fashion into multiple isoforms, and which are responsible for an enormous breadth of cellular functions. These functions are related to the regulation of actin, microtubules, intermediate filaments, intracellular organelles, cell adhesions and signalling processes during the development and maintenance of a wide variety of tissues. To gain a deeper understanding of this enormous functional diversity, invertebrate genetic model organisms, such as the fruit fly Drosophila, can be used to develop concepts and mechanistic paradigms that can inform the investigation in higher animals or humans. Here we provide a comprehensive overview of our current knowledge of the Drosophila spectraplakin Short stop (Shot). We describe its functional domains and isoforms and compare them with those of the mammalian spectraplakins dystonin and MACF1. We then summarise its roles during the development and maintenance of the nervous system, epithelia, oocytes and muscles, taking care to compare and contrast mechanistic insights across these functions in the fly, but especially also with related functions of dystonin and MACF1 in mostly mammalian contexts. We hope that this review will improve the wider appreciation of how work on Drosophila Shot can be used as an efficient strategy to promote the fundamental concepts and mechanisms that underpin spectraplakin functions, with important implications for biomedical research into human disease.


Subject(s)
Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Microfilament Proteins/metabolism , Animals , Axon Guidance , Drosophila Proteins/chemistry , Drosophila Proteins/genetics , Mammals/metabolism , Microfilament Proteins/chemistry , Microfilament Proteins/genetics , Sequence Homology, Amino Acid , Synapses/metabolism
9.
J Biol Chem ; 291(48): 25120-25132, 2016 Nov 25.
Article in English | MEDLINE | ID: mdl-27702999

ABSTRACT

Cereblon (CRBN) is a substrate receptor of the E3 ubiquitin ligase complex that is highly conserved in animals and plants. CRBN proteins have been implicated in various biological processes such as development, metabolism, learning, and memory formation, and their impairment has been linked to autosomal recessive non-syndromic intellectual disability and cancer. Furthermore, human CRBN was identified as the primary target of thalidomide teratogenicity. Data on functional analysis of CRBN family members in vivo, however, are still scarce. Here we identify Ohgata (OHGT), the Drosophila ortholog of CRBN, as a regulator of insulin signaling-mediated growth. Using ohgt mutants that we generated by targeted mutagenesis, we show that its loss results in increased body weight and organ size without changes of the body proportions. We demonstrate that ohgt knockdown in the fat body, an organ analogous to mammalian liver and adipose tissue, phenocopies the growth phenotypes. We further show that overgrowth is due to an elevation of insulin signaling in ohgt mutants and to the down-regulation of inhibitory cofactors of circulating Drosophila insulin-like peptides (DILPs), named acid-labile subunit and imaginal morphogenesis protein-late 2. The two inhibitory proteins were previously shown to be components of a heterotrimeric complex with growth-promoting DILP2 and DILP5. Our study reveals OHGT as a novel regulator of insulin-dependent organismic growth in Drosophila.


Subject(s)
Drosophila Proteins , Insulins , Signal Transduction/physiology , Adaptor Proteins, Signal Transducing , Animals , Cell Line , Drosophila Proteins/genetics , Drosophila Proteins/metabolism , Drosophila melanogaster , Gene Knockdown Techniques , Humans , Insulins/genetics , Insulins/metabolism , Peptide Hydrolases/genetics , Peptide Hydrolases/metabolism , Ubiquitin-Protein Ligases
10.
Elife ; 52016 08 08.
Article in English | MEDLINE | ID: mdl-27501441

ABSTRACT

The mechanisms regulating synapse numbers during development and ageing are essential for normal brain function and closely linked to brain disorders including dementias. Using Drosophila, we demonstrate roles of the microtubule-associated protein Tau in regulating synapse numbers, thus unravelling an important cellular requirement of normal Tau. In this context, we find that Tau displays a strong functional overlap with microtubule-binding spectraplakins, establishing new links between two different neurodegenerative factors. Tau and the spectraplakin Short Stop act upstream of a three-step regulatory cascade ensuring adequate delivery of synaptic proteins. This cascade involves microtubule stability as the initial trigger, JNK signalling as the central mediator, and kinesin-3 mediated axonal transport as the key effector. This cascade acts during development (synapse formation) and ageing (synapse maintenance) alike. Therefore, our findings suggest novel explanations for intellectual disability in Tau deficient individuals, as well as early synapse loss in dementias including Alzheimer's disease.


Subject(s)
Drosophila Proteins/genetics , Drosophila melanogaster/genetics , JNK Mitogen-Activated Protein Kinases/genetics , Kinesins/genetics , Microfilament Proteins/genetics , Synapses/genetics , tau Proteins/genetics , Alzheimer Disease/genetics , Alzheimer Disease/metabolism , Alzheimer Disease/pathology , Animals , Axonal Transport , Brain/cytology , Brain/growth & development , Brain/metabolism , Cell Movement , Dementia/genetics , Dementia/metabolism , Dementia/pathology , Disease Models, Animal , Drosophila Proteins/metabolism , Drosophila melanogaster/growth & development , Drosophila melanogaster/metabolism , Gene Expression Regulation, Developmental , Humans , JNK Mitogen-Activated Protein Kinases/metabolism , Kinesins/metabolism , Microfilament Proteins/metabolism , Microtubules/metabolism , Microtubules/ultrastructure , Neurogenesis/genetics , Neurons/metabolism , Neurons/ultrastructure , Protein Transport , Signal Transduction , Synapses/metabolism , Synapses/ultrastructure , tau Proteins/metabolism
11.
Brain Res Bull ; 126(Pt 3): 226-237, 2016 09.
Article in English | MEDLINE | ID: mdl-27530065

ABSTRACT

Axons are the cable-like protrusions of neurons which wire up the nervous system. Polar bundles of microtubules (MTs) constitute their structural backbones and are highways for life-sustaining transport between proximal cell bodies and distal synapses. Any morphogenetic changes of axons during development, plastic rearrangement, regeneration or degeneration depend on dynamic changes of these MT bundles. A key mechanism for implementing such changes is the coordinated polymerisation and depolymerisation at the plus ends of MTs within these bundles. To gain an understanding of how such regulation can be achieved at the cellular level, we provide here an integrated overview of the extensive knowledge we have about the molecular mechanisms regulating MT de/polymerisation. We first summarise insights gained from work in vitro, then describe the machinery which supplies the essential tubulin building blocks, the protein complexes associating with MT plus ends, and MT shaft-based mechanisms that influence plus end dynamics. We briefly summarise the contribution of MT plus end dynamics to important cellular functions in axons, and conclude by discussing the challenges and potential strategies of integrating the existing molecular knowledge into conceptual understanding at the level of axons.


Subject(s)
Axons/metabolism , Microtubules/metabolism , Animals , Humans
12.
FEBS Lett ; 590(7): 971-81, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26950647

ABSTRACT

Drosophila Ceramide Synthase (CerS) Schlank regulates both ceramide synthesis and fat metabolism. Schlank contains a catalytic lag1p motif and, like many CerS in other species, a homeodomain of unknown function. Here, we show that the Drosophila CerS Schlank is imported into the nucleus and requires two nuclear localization signals (NLSs) within its homeodomain and functional Importin-ß import machinery. Expression of Schlank variants containing the homeodomain without functional lag1p motif rescued the fat metabolism phenotype of schlank mutants whereas a variant with a mutated NLS site did not rescue. Thus, the homeodomain of Schlank is involved in the regulation of lipid metabolism independent of the catalytic lag1p motif.


Subject(s)
Cell Nucleus/metabolism , Drosophila Proteins/metabolism , Drosophila melanogaster/metabolism , Fat Body/metabolism , Lipid Metabolism , Nuclear Localization Signals/metabolism , Sphingosine N-Acyltransferase/metabolism , Active Transport, Cell Nucleus , Amino Acid Motifs , Amino Acid Substitution , Animals , Animals, Genetically Modified , Catalytic Domain , Cell Line , Cell Nucleus/enzymology , Drosophila Proteins/antagonists & inhibitors , Drosophila Proteins/chemistry , Drosophila Proteins/genetics , Drosophila melanogaster/cytology , Drosophila melanogaster/genetics , Fat Body/cytology , Fat Body/enzymology , Green Fluorescent Proteins/genetics , Green Fluorescent Proteins/metabolism , Homeodomain Proteins/chemistry , Homeodomain Proteins/genetics , Homeodomain Proteins/metabolism , Mutation , Nuclear Localization Signals/chemistry , Nuclear Localization Signals/genetics , Peptide Fragments/antagonists & inhibitors , Peptide Fragments/chemistry , Peptide Fragments/genetics , Peptide Fragments/metabolism , Protein Interaction Domains and Motifs , RNA Interference , Recombinant Fusion Proteins/chemistry , Recombinant Fusion Proteins/metabolism , Sphingosine N-Acyltransferase/antagonists & inhibitors , Sphingosine N-Acyltransferase/chemistry , Sphingosine N-Acyltransferase/genetics , beta Karyopherins/antagonists & inhibitors , beta Karyopherins/genetics , beta Karyopherins/metabolism
13.
Dev Cell ; 28(6): 711-26, 2014 Mar 31.
Article in English | MEDLINE | ID: mdl-24697899

ABSTRACT

Calcineurin is a heteromeric Ca(2+)-dependent serine/threonine phosphatase. It dephosphorylates the transcription factor nuclear factor of activated T cells (NFAT) in the cytoplasm, which subsequently undergoes nuclear translocation. NFAT regulates numerous biological processes, including inflammatory T cell responses and cardiac development. Our study identifies the Cysteine-Rich with EGF-Like Domains 1 (Creld1) gene as a regulator of calcineurin/NFATc1 signaling. We show that Creld1 is sufficient to promote NFATc1 dephosphorylation and translocation to the nucleus. Creld1 is contained in a joint protein complex with the regulatory subunit of calcineurin, CnB, thereby controlling calcineurin function. Localization of Creld1 at the endoplasmic reticulum (ER) is important to exert its action on calcineurin. By using Creld1KO mice, we demonstrate that Creld1 is essential for heart development. Creld1 function is required for the VEGF-dependent proliferation of endocardial cells by promoting the expression of NFATc1 target-genes. Collectively, our study identifies Creld1 as an important regulator of calcineurin/NFATc1 signaling.


Subject(s)
Calcineurin/metabolism , Cell Adhesion Molecules/physiology , Endocardium/cytology , Extracellular Matrix Proteins/physiology , Heart/embryology , NFATC Transcription Factors/metabolism , Animals , Blotting, Western , Calcineurin/genetics , Calcium/metabolism , Cell Differentiation , Cell Nucleus/metabolism , Cell Proliferation , Cells, Cultured , Cytoplasm/metabolism , Endocardium/metabolism , Endoplasmic Reticulum/metabolism , Heart/physiology , Humans , Immunoprecipitation , Mice , Mice, Knockout , Mutation/genetics , NFATC Transcription Factors/genetics , NIH 3T3 Cells , Phosphorylation , Protein Transport , RNA, Messenger/genetics , Real-Time Polymerase Chain Reaction , Reverse Transcriptase Polymerase Chain Reaction , Signal Transduction , Vascular Endothelial Growth Factor A
14.
Gene Expr Patterns ; 11(8): 501-10, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21907829

ABSTRACT

Schlank is a member of the highly conserved ceramide synthase family and controls growth and body fat in Drosophila. Ceramide synthases are key enzymes in the sphingolipid de novo synthesis pathway. Ceramide synthase proteins and the (dihydro)ceramide produced are involved in a variety of biological processes among them apoptosis and neurodegeneration. The full extent of their involvement in these processes will require a precise analysis of the distribution and expression pattern of ceramide synthases. Paralogs of the ceramide synthase family have been found in all eukaryotes studied, however the mRNA and protein expression patterns have not yet been analysed systematically. In this study, we use antibodies that specifically recognize Schlank, a schlank mRNA probe and an endogenous schlank promoter driven LacZ reporter line to reveal the expression pattern of Schlank throughout embryogenesis. We found that Schlank is expressed in all embryonic epithelia during embryogenesis including the developing epidermis and the gastrointestinal tract. In addition, Schlank is upregulated in the developing central (CNS) and peripheral nervous system (PNS). Co-staining experiments with neuronal and glial markers revealed specific expression of Schlank in glial and neuronal cells of the CNS and PNS.


Subject(s)
Central Nervous System/embryology , Drosophila Proteins/biosynthesis , Embryo, Nonmammalian/embryology , Gene Expression Regulation, Developmental/physiology , Gene Expression Regulation, Enzymologic/physiology , Intestines/embryology , Nerve Tissue Proteins/biosynthesis , Oxidoreductases/biosynthesis , Peripheral Nervous System/embryology , Animals , Central Nervous System/cytology , Central Nervous System/enzymology , Drosophila Proteins/genetics , Drosophila melanogaster , Embryo, Nonmammalian/cytology , Embryo, Nonmammalian/enzymology , Embryonic Development/physiology , Intestines/cytology , Intestines/enzymology , Nerve Tissue Proteins/genetics , Organ Specificity/physiology , Oxidoreductases/genetics , Peripheral Nervous System/cytology , Peripheral Nervous System/enzymology , RNA, Messenger/biosynthesis , Sphingosine N-Acyltransferase
15.
Biomol Concepts ; 1(5-6): 411-22, 2010 Dec 01.
Article in English | MEDLINE | ID: mdl-25962014

ABSTRACT

The ceramide synthase (CerS) gene family comprises a group of highly conserved transmembrane proteins, which are found in all studied eukaryotes. The key feature of the CerS proteins is their role in ceramide synthase activity. Therefore, their original name 'longevity assurance gene (Lass) homologs', after the founding member, the yeast longevity assurance gene lag1, was altered to 'CerS'. All CerS have high sequence similarity in a domain called LAG1 motif and a subset of CerS proteins is predicted to contain a Homeobox (Hox) domain. These domains could be the key to the multiple roles CerS have. CerS proteins play a role in diverse biological processes such as proliferation, differentiation, apoptosis, stress response, cancer, and neurodegeneration. In this review, we focus on CerS structure and biological function with emphasis of biological functions in the widely used model systems Caenorhabditis elegans and Drosophila melanogaster. Also, we focus on the accumulating data suggesting a role for CerS in lipid homeostasis.

16.
EMBO J ; 28(23): 3706-16, 2009 Dec 02.
Article in English | MEDLINE | ID: mdl-19834458

ABSTRACT

Ceramide synthases are highly conserved transmembrane proteins involved in the biosynthesis of sphingolipids, which are essential structural components of eukaryotic membranes and can act as second messengers regulating tissue homeostasis. However, the role of these enzymes in development is poorly understood due to the lack of animal models. We identified schlank as a new Drosophila member of the ceramide synthase family. We demonstrate that schlank is involved in the de novo synthesis of a broad range of ceramides, the key metabolites of sphingolipid biosynthesis. Unexpectedly, schlank mutants also show reduction of storage fat, which is deposited as triacylglyerols in the fat body. We found that schlank can positively regulate fatty acid synthesis by promoting the expression of sterol-responsive element-binding protein (SREBP) and SREBP-target genes. It further prevents lipolysis by downregulating the expression of triacylglycerol lipase. Our results identify schlank as a new regulator of the balance between lipogenesis and lipolysis in Drosophila. Furthermore, our studies of schlank and the mammalian Lass2 family member suggest a novel role for ceramide synthases in regulating body fat metabolism.


Subject(s)
Drosophila Proteins/physiology , Drosophila melanogaster/enzymology , Drosophila melanogaster/growth & development , Multigene Family/physiology , Oxidoreductases/physiology , Adipose Tissue/enzymology , Adipose Tissue/growth & development , Adipose Tissue/metabolism , Animals , Animals, Genetically Modified , Conserved Sequence , Drosophila Proteins/genetics , Drosophila melanogaster/embryology , Drosophila melanogaster/genetics , Female , Larva/enzymology , Larva/genetics , Larva/growth & development , Larva/metabolism , Lipogenesis/physiology , Lipolysis/physiology , Male , Oxidoreductases/genetics
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