Internally gas-cooled radiofrequency applicators as an alternative to conventional radiofrequency and microwave ablation devices: an in vivo comparison.

PURPOSE: To test the efficacy of internally CO2-cooled radiofrequency (RF) ablation in vivo and to compare its effectiveness to a standard water-cooled RF probe and to a gas-cooled microwave (MW) device. METHOD AND MATERIALS: 49 ablations were performed on 15 pigs under general anesthesia using 15G monopolar CO2-cooled RF applicators, 17G monopolar water-cooled RF applicators and 15G internally CO2-cooled microwave devices. The power of the MW device was 45W, the current of the gas-cooled RF device was 1200-1600mA. At the water-cooled RF probe, maximum power of 200W was set. Ablation time was 15min. The short and long axes of the ablation zone were measured. Histological analyses and NADH-staining were performed. The diameters and the ablation volumes were compared using an analysis of variance. RESULTS: No spots of untreated tissue were observed close to the cooled needle track in any of the ablation zones. The largest short axis diameter was 3.4±0.5cm achieved with the gas-cooled monopolar applicator. With the water-cooled applicators, short axis diameter was significantly smaller, reaching 2.5±0.4cm. Gas-cooled MW probes achieved 2.9±1.0cm. The largest ablation volume was 31.5±12ml (gas-cooled RF), and the smallest was 12.7±4ml (water-cooled RF). Short/long axis ratio was largest for gas-cooled RF probes with 0.73±0.08 versus 0.64±0.04 for the water-cooled probes and 0.49±0.25 for the microwave applicator. CONCLUSION: Gas-cooled RF applicators may have a higher potential for effective destruction of liver lesions than comparable water-cooled RF systems, and may be an alternative to standard RF and MW ablation devices.

Evaluation of the hemostatic and coagulation effects of AUTO CUT and DRY CUT using a computer-controlled cutting system.

OBJECTIVES: To evaluate a newly developed computer-controlled cutting system for the generation of standardized resections and to systematically compare the hemostatic properties and tissue effect of 2 cutting modes, namely, AUTO CUT and DRY CUT used in urologic procedures. METHODS: An isolated perfused kidney model was used to assess blood loss and coagulation depth after resection of tissue specimens of standardized geometry, size, and cutting velocity with a resection loop. Three different effect settings (E1, E3, and E6; 200 W) of the electrosurgical modes AUTO CUT and DRY CUT were compared. Blood loss was determined semiquantitatively by weighing a swab before and after placing it onto the resection area. The coagulation depth was estimated microscopically on cross sections. RESULTS: The computer-controlled cutting system creates resections of standardized geometry and size with a high reproducibility. An effect level-dependent increase in hemostasis and coagulation depth could be demonstrated with the cutting modes DRY CUT and AUTO CUT using this computer-controlled cutting system. The hemostatic effect with DRY CUT is significantly more pronounced than with AUTO CUT (E1, E3: P < .0001, E6: P = .004), and the coagulation is significantly deeper (E1, E3, E6: P < .0001). CONCLUSIONS: The computer-controlled cutting system creating reproducible resections in combination with the isolated perfused kidney model offers the possibility to systematically investigate bleeding rate and coagulation depth. The stronger hemostatic properties of the DRY CUT mode are more favorable for urologic interventions requiring a higher hemostatic effect than the AUTO CUT mode.

Increased ablation zones using a cryo-based internally cooled bipolar RF applicator in ex vivo bovine liver.

PURPOSE: To evaluate the feasibility of ex vivo ablation implementing a cryo-based internally cooled bipolar radiofrequency (RF) applicator and to determine the influence of power and gas pressure on the size and shape of the resulting ablation zones. MATERIALS AND METHODS: Two hundred twenty-five ablations were performed using a custom-built internally CO2-cooled bipolar cryo RF applicator in ex vivo bovine livers. The active tip of the applicator was 55 mm long. RF power (32-50 watts) and gas pressure of cooling medium (500-600 psi) were varied independently. Power was applied in continuous mode. Control group experiments were carried out solely using the RF function at 32, 40, and 50 watts. Ablation duration was 15 minutes for all applications. Experiments were repeated 5 times for all parameter combinations. Short and long axes of the induced white ablation zone were macroscopically assessed. The ablation zone was referred to as homogeneous if complete ablation was observed without spots of untreated tissue. The short axis diameters for the simultaneous application of cryo and RF function were analyzed using a multiple linear regression analysis. An unpaired Mann-Whitney U test was used to analyze the differences between the short axes with RF alone and RF using cryo cooling. RESULTS: All ablation zones were homogeneous. Using simultaneous RF ablation function and gas cooling with a single applicator, the long axes of the ablation zones ranged between 42 +/- 2 mm (mean +/- SD) and 59 +/- 5 mm, the short axes between 24 +/- 1 and 44 +/- 1 mm, depending on the parameter combination. At a stable gas pressure level, short axes increased with rising power levels and decreased after reaching a maximum. The maxima of the short axis increased with higher gas pressure levels and were shifted to higher power values. Optimal parameter settings were 46 to 50 watts and 600 psi gas pressure, resulting in a short axis of 44 +/- 1 mm. Short axis weakly correlated with gas pressure (r2 = 0.10) and power (r2 = 0.34) alone, whereas the correlation was r2 = 0.76 for the combined factors. Without cooling, short axis diameters were significantly shorter (P < 0.05), ranging between 13 +/- 2 mm at 50 watts and 15 +/- 2 mm at 32 watts. CONCLUSION: The results of this initial ex vivo study show that the combined cryo RF ablation device allows for large ablation volumes using a single needle, which is superior to RF ablation alone.