ABSTRACT
Chronic gamma-irradiation (1 Gy, 100 mR/hr) increases cell titre in rat thymus and decreases glucose transport activation by phorbol ester. Animals kept in the Chernobyl evacuation zone over a period of 30 days exhibit reduced thymus cellularity and decreased PMA (200 nM) stimulation of glucose uptake by thymus cells.
Subject(s)
Accidents, Occupational , Environmental Exposure , Glucose/pharmacokinetics , Nuclear Reactors , Thymus Gland/radiation effects , Animals , Biological Transport/radiation effects , Male , Models, Biological , Phorbol Esters/pharmacology , Protein Kinase C/metabolism , Rats , Thymus Gland/cytology , Thymus Gland/metabolism , Time FactorsSubject(s)
Amiloride/pharmacology , Cell Death/radiation effects , Interphase/radiation effects , Tetradecanoylphorbol Acetate/pharmacology , Thymus Gland/radiation effects , Animals , Cell Death/drug effects , Chromatin , DNA/metabolism , Interphase/drug effects , Rats , Thymus Gland/cytology , Thymus Gland/drug effectsABSTRACT
The method of luminol-dependent chemiluminescence was used to detect oxygen activation during interaction of HLA antibodies with HLA antigens expressed on lymphocyte surface. Introduction of specific anti-HLA serum into the lymphocyte suspension leads to a rapid decrease of intensity in chemiluminescence intensity follows. The maximum yield of induced chemiluminescence in case of using a specific antiserum is significantly lower than in the control. The method could be used for detection of HLA antigens on lymphocyte surface.
Subject(s)
Antigen-Antibody Reactions/physiology , HLA Antigens/immunology , Isoantibodies/immunology , Lymphocytes/metabolism , Oxygen/blood , Cell Membrane/immunology , Cell Membrane/metabolism , Culture Media , Humans , In Vitro Techniques , Luminescent Measurements , Luminol/pharmacology , Lymphocytes/drug effects , Lymphocytes/immunologyABSTRACT
Using the fluorescent probe and membrane filtration methods it has been shown that 3 days after gamma-irradiation of rats with a dose of 1 Gy microviscosity of membranes increases and their Ca2(+)-transport function decreases with a simultaneous increase in the affinity of Ca2(+)-pump for calcium. In 15 days, the characteristics under study do not differ from those of the intact controls.