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1.
Radiats Biol Radioecol ; 33(4): 521-4, 1993.
Article in Russian | MEDLINE | ID: mdl-8401872

ABSTRACT

Chronic gamma-irradiation (1 Gy, 100 mR/hr) increases cell titre in rat thymus and decreases glucose transport activation by phorbol ester. Animals kept in the Chernobyl evacuation zone over a period of 30 days exhibit reduced thymus cellularity and decreased PMA (200 nM) stimulation of glucose uptake by thymus cells.


Subject(s)
Accidents, Occupational , Environmental Exposure , Glucose/pharmacokinetics , Nuclear Reactors , Thymus Gland/radiation effects , Animals , Biological Transport/radiation effects , Male , Models, Biological , Phorbol Esters/pharmacology , Protein Kinase C/metabolism , Rats , Thymus Gland/cytology , Thymus Gland/metabolism , Time Factors
3.
Gematol Transfuziol ; 35(12): 20-3, 1990 Dec.
Article in Russian | MEDLINE | ID: mdl-2083828

ABSTRACT

The method of luminol-dependent chemiluminescence was used to detect oxygen activation during interaction of HLA antibodies with HLA antigens expressed on lymphocyte surface. Introduction of specific anti-HLA serum into the lymphocyte suspension leads to a rapid decrease of intensity in chemiluminescence intensity follows. The maximum yield of induced chemiluminescence in case of using a specific antiserum is significantly lower than in the control. The method could be used for detection of HLA antigens on lymphocyte surface.


Subject(s)
Antigen-Antibody Reactions/physiology , HLA Antigens/immunology , Isoantibodies/immunology , Lymphocytes/metabolism , Oxygen/blood , Cell Membrane/immunology , Cell Membrane/metabolism , Culture Media , Humans , In Vitro Techniques , Luminescent Measurements , Luminol/pharmacology , Lymphocytes/drug effects , Lymphocytes/immunology
4.
Radiobiologiia ; 30(1): 54-6, 1990.
Article in Russian | MEDLINE | ID: mdl-2156281

ABSTRACT

Using the fluorescent probe and membrane filtration methods it has been shown that 3 days after gamma-irradiation of rats with a dose of 1 Gy microviscosity of membranes increases and their Ca2(+)-transport function decreases with a simultaneous increase in the affinity of Ca2(+)-pump for calcium. In 15 days, the characteristics under study do not differ from those of the intact controls.


Subject(s)
Muscle, Smooth, Vascular/radiation effects , Animals , Calcium Channels/physiology , Calcium Channels/radiation effects , Calcium Channels/ultrastructure , Cell Membrane/physiology , Cell Membrane/radiation effects , Cell Membrane/ultrastructure , Dose-Response Relationship, Radiation , Gamma Rays , Male , Membrane Fluidity/physiology , Membrane Fluidity/radiation effects , Muscle, Smooth, Vascular/physiology , Muscle, Smooth, Vascular/ultrastructure , Rats , Rats, Inbred Strains , Time Factors , Viscosity
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