ABSTRACT
The optimal conditions for isolation of naphazoline from naphthyzin preparations and biological fluids with chloroform at pH 9.18 are described. The compound of interest was identified with the use of color and precipitation reactions, IR and UV spectroscopy, thin-layer and gas chromatography, and chemical methods including high performance liquid chromatography, chromatodensitometry, and UV spectroscopy. The results obtained by the three methods are comparable.
Subject(s)
Naphazoline , Substance-Related Disorders/diagnosis , Adrenergic alpha-Agonists/blood , Adrenergic alpha-Agonists/pharmacology , Adrenergic alpha-Agonists/urine , Animals , Body Fluids , Chromatography, High Pressure Liquid/methods , Densitometry/methods , Forensic Toxicology/methods , Gas Chromatography-Mass Spectrometry/methods , Humans , Mice , Naphazoline/blood , Naphazoline/pharmacology , Naphazoline/urine , Substance Abuse Detection/methodsABSTRACT
The objective of the present work was to study conditions for isolation of ketorolac and diclofenac from biological fluids. A method of their extraction with a mixture of organic solvents has been developed and the conditions for the identification of these compounds are proposed with the use of high performance liquid chromatography (HPLC), UV spectroscopy, and gas chromatography with electron capture detection (GC/ECD). The possibilities of using HPLC, UV spectrometry, and GC/ECD for quantitative determination of ketorolac and diclofenac are illustrated.
Subject(s)
Diclofenac/blood , Ketorolac/blood , Chromatography, Gas/methods , Chromatography, High Pressure Liquid/methods , Humans , Sensitivity and Specificity , Spectrophotometry, Ultraviolet/methodsABSTRACT
Optimal conditions for the separation of diclofenac and ketorolac in the presence of other pharmaceutical products and narcotic substances are described with the help of gas and high performance liquid chromatography. The detection limits for individual compounds were established and the characteristic ions were identified. The possibility of application of IR- and UV-spectroscopy for the analysis of selected narcotic and non-narcotic analgesics was evaluated.
Subject(s)
Anti-Inflammatory Agents/analysis , Forensic Sciences/methods , Anti-Inflammatory Agents/chemistry , Chromatography, Gas/methods , Chromatography, High Pressure Liquid/methods , Mass Spectrometry/methods , Narcotics/analysis , Narcotics/chemistryABSTRACT
The conditions of butorphanol isolation from biological fluids were studied. The method of its extraction with the mix of organic solvents by pH 12 was proposed. How to identify butorphanol with the methods of thin-layer chromatography, ultraviolet spectrometry, high-performance liquid chromatography, gas chromatography with a detector of electron capture, chromato-mass spectrometry was developed. Possibility of use ultraviolet spectrometry for quantitative assessment of butorphanol was shown.
Subject(s)
Analgesics, Opioid , Butorphanol , Forensic Toxicology/methods , Pharmaceutical Preparations , Substance Abuse Detection/methods , Analgesics, Opioid/analysis , Analgesics, Opioid/blood , Analgesics, Opioid/urine , Animals , Butorphanol/analysis , Butorphanol/blood , Butorphanol/urine , Chromatography, High Pressure Liquid , Chromatography, Thin Layer , Pharmaceutical Preparations/chemistry , Pharmaceutical Preparations/standards , RatsABSTRACT
The conditions of doxilamine isolation from biological fluids are studied. The method of its extraction with a mixture of organic solvents in pH 9 is proposed. Identification of doxilamine with techniques of thin layer chromatography, UV-, IR-spectroscopy, chromato-mass-spectrometry, densitometry, gas-liquid chromatography is described. UV-spectrometry, gas chromatography and densitometry can be used for quantitation of doxilamine.