ABSTRACT
On the basis of data obtained by the biological characterization and electrophoretic typing of Acinetobacter sp. cultures by the spectra of membrane proteins epidemiological relationships, routes of transmission and specific features of the circulation of strains causing purulent inflammatory diseases have been determined.
Subject(s)
Acinetobacter Infections/epidemiology , Acinetobacter/chemistry , Bacterial Proteins/analysis , Electrophoresis, Polyacrylamide Gel/methods , Acinetobacter/classification , Acinetobacter/drug effects , Acinetobacter/isolation & purification , Acinetobacter Infections/diagnosis , Acinetobacter Infections/etiology , Acinetobacter Infections/microbiology , Anti-Bacterial Agents/pharmacology , Burns/complications , Burns/microbiology , Humans , Microbial Sensitivity Tests , Prospective Studies , Russia/epidemiologyABSTRACT
Glucose nonfermenting gram-negative bacteria were electrophoretically typed for the extracellular protein spectra. The method permits differentiation on the generic level the cultures of such bacteria, characterized by atypical biochemistry, within 10 hours.
Subject(s)
Gram-Negative Bacteria/classification , Electrophoresis , Glucose/metabolism , Gram-Negative Bacteria/enzymologyABSTRACT
A high degree of similarity (exceeding 90%) between the electrophoregrams of the exoproducts of 31 Pseudomonas strains, including the standard species P. aeruginosa, P. maltophilia, P. putida, P. alcaligenes, P. testosteroni, P. diminuta, P. stutseri, P. fluorescens, has been shown by the method of electrophoresis in polyacrylamide gel.
Subject(s)
Bacterial Outer Membrane Proteins/analysis , Pseudomonas/analysis , Bacterial Outer Membrane Proteins/classification , Bacterial Outer Membrane Proteins/isolation & purification , Electrophoresis, Polyacrylamide Gel , Peptide Hydrolases/analysis , Pseudomonas/classification , Serotyping , SpectrophotometryABSTRACT
Outer membrane proteins have been studied in 76 Salmonella strains isolated from various sources and differing in their sensitivity to antibiotics, the presence of R-plasmids and a number of enzymatic properties. The outer membranes have been isolated by the modified method of L. W. Coulton and D. T. F. Wan. The study of the isolated proteins has been carried out by means of gel and disc electrophoresis in polyacrylamide gel with sodium dodecyl sulfate according to K. Weber and M. Osborn. The composition of the proteinograms thus obtained has revealed the presence of essential differences between hospital strains and cultures isolated from animals and from the environment in sporadic infections, as well as between strains belonging to different serovars. The possibility of using the characterization of outer membrane proteins of salmonellae in epidemiological investigations is discussed.