ABSTRACT
As the result of our investigations, newly isolated B. pertussis and B. bronchiseptica strains were studied. The results of these investigations showed that B. pertussis strains isolated under the conditions of immunoprophylaxis were characterized by sufficient stability of the main phenotypical properties which determined their pathogenicity: B. pertussis toxin, fimbrial agglutinogens and filamentous hemagglutinin. At the same time B. bronchiseptica strains isolated from animals proved to be phenotypically variable both in vivo and in the process of in vitro passage.
Subject(s)
Bordetella/isolation & purification , Adolescent , Agglutinins/analysis , Animal Husbandry , Animals , Bordetella/analysis , Bordetella/pathogenicity , Bordetella pertussis/analysis , Bordetella pertussis/isolation & purification , Bordetella pertussis/pathogenicity , Child , Child, Preschool , Fimbriae, Bacterial/chemistry , Hemagglutinins/analysis , Humans , Infant , Pertussis Toxin , Phenotype , Species Specificity , Swine/microbiology , Ukraine , Virulence Factors, Bordetella/analysisABSTRACT
A number of repeated sequences was identified in the chromosome of Bordetella pertussis by the electron microscopic analysis of the chromosomal DNA of this microorganism. One of the sequences was cloned on the vector plasmid pHC79. It is shown to consist of two elements RSBP1 and RSBP2. The first elements is probably identical to an RS-element described previously. The cloned RSBP1 element is shown to stimulate the deletion formation in the genome of the plasmid pMKII and is able to transpose into the chromosome of Escherichia coli. The latter properties permit one to classify RSBP1 as an element belonging to a class of migrating genetical elements.
Subject(s)
Bordetella pertussis/genetics , DNA Transposable Elements , Chromosomes, Bacterial/ultrastructure , Cloning, Molecular , Escherichia coli/genetics , Genes, Bacterial , Nucleic Acid Hybridization , Plasmids , Repetitive Sequences, Nucleic Acid , Restriction MappingABSTRACT
To study the structural arrangement of the chromosomal region containing vir genes of Bordetella pertussis the corresponding 15 kb fragment of Bordetella pertussis chromosomal DNA has been cloned. The sequence homology to an earlier characterized Bordetella pertussis genetical element RSBP1 and flanked by two 400 bp inverted repeats has been shown to be located at an end of a BamHI fragment. The restriction map of Bordetella pertussis 475 coincides with the previously published maps of Bordetella pertussis Tohama and 18323 permitting one to conclude the definite conservatism of the cloned sequence. The preliminary data obtained make possible mapping of the RSBP1 homologous sequence adjacent to adenylate cyclase, agglutinin 2 and pertussis toxin genes. The possible role of RSBP1 elements in the regulation of Bordetella virulence is suggested.