[Antiviral activity of vegetable triterpens, their derivatives and ribavirin phosphonates]. / Protivovirusnaia aktivnost' triterpenov rastitel'nogo proiskhozhdeniia, ikh proizvodnykh i fosfonatov ribavirina.

The purpose of the study was to investigate, in the Vero cell culture, the antiviral activity of vegetable tritrpens derivatives and ribavirin analogues against the viruses of measles, herpes simple (type 1), cytomegaloviruses and filoviruses. The toxicity and antiviral activity of compounds were determined after coloring of cells with crystal violate. Additionally, the combined action of triterpens' derivatives and ribavirin was investigated. The studied compounds showed relatively low antiviral activity, nonetheless, further research of vegetable triterpens and their derivatives as well as ribavirin analogues would be promising.

[The detection of antibodies to the trophozoite antigens of Lamblia by an immunoenzyme method]. / Vyiavlenie antitel k antigenam trofozoitov liamblii immunofermentnym metodom.

Whether purified antigens of Lamblia intestinalis trophozoites can be used to detect these antibodies by immunoassay. The drugs of immunodominant Lamblia antigens were prepared by anion-exchange chromatography of solubilized trophozoite components and they are mainly presented by proteins having molecular weights of 70, 56, and 49 kD. Immunoassay using these antigens revealed antibodies to Lamblia trophozoite antigens in sera of 87.6% of patients with lambliasis (its diagnosis was established on the basis of microscopic data on the duodenal content) and only in 16.2% of clinically healthy blood donors. Twenty six sera from patients with trichomoniasis having high levels of antibodies to trichomonad antigens were studied to evaluate the specificity of this method for detection of antibodies. It has been found that the proportion of subjects in this group who have also antibodies to Lamblia antigens does not greatly differ from that of healthy blood donors (19.2 and 16.2, respectively).

[The seroepidemiology of toxocariasis in an opisthorchiasis focus in the western Siberian region]. / Seroepidemiologiia toksokaroza v ochage opistorkhoza zapadno-sibirskogo regiona.

Seroprevalence to Toxocara antigens was studied among Novosibirsk inhabitants and a focus of toxocariasis was found in the southern regions of west Siberia. Analyzing the sera of opisthorchiasis patients revealed antibodies to Toxocara antigens, with high titers (1:800 or more) in 1.80% of cases. Immunoblotting of these sera and those from patients with toxocariasis and opisthorchiasis, which were seropositive in one of the above helminths showed that there were mixed human infections with Toxocara and Opisthorchis in this area.