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1.
Bull Exp Biol Med ; 174(6): 707-710, 2023 Apr.
Article in English | MEDLINE | ID: mdl-37157044

ABSTRACT

We studied the effects of age and suntan on the expression of necroptosis signaling molecules (RIPK1, RIPK3, and MLKL kinases) and first TNF receptor (TNFR1) in isolated skin cells from women undergoing facelift surgery. In women above 50 years, the expression of the TNFR1, kinases RIPK1, RIPK3, and MLKL, the phosphorylated forms of these kinases was significantly (p<0.05) increased in comparison with the corresponding parameters in women under 30 years. The expression of all necroptosis proteins and TNFR1 in women with suntan was significantly (p<0.05) higher than in those without tan. Cells from the surgical material were incubated with TNFα to determine the level of induced necroptosis. In women aged >50 years and women with suntan, the expression of phosphorylated forms of kinases was significantly increased, which attested to necroptosis activation. This study allowed identifying the targets on skin cells for prevention of necrosis and inflammation after facelift surgery.


Subject(s)
Protein Kinases , Suntan , Female , Humans , Protein Kinases/metabolism , Protein Kinases/pharmacology , Receptors, Tumor Necrosis Factor, Type I/genetics , Receptors, Tumor Necrosis Factor, Type I/metabolism , Necroptosis , Signal Transduction , Second Messenger Systems , Apoptosis
2.
Bull Exp Biol Med ; 167(6): 728-731, 2019 Oct.
Article in English | MEDLINE | ID: mdl-31655991

ABSTRACT

The study was aimed at assessing the role of chemokines in inflammatory changes in tissue following abdominoplasty. The levels of neutrophil-coupled chemokines and their receptors in the serum and blood cells, as well as in cells isolated from the subcutaneous adipose tissue sampled during abdominoplasty were compared in patients with obesity and normal body weight. The levels of chemokines CCL3, CCL3, and CCL5 in blood serum and expression of chemokine receptor CXCR2 and CXCR6 on blood neutrophils were significantly higher (p<0.05) in obese patients in comparison with patients with normal body weight. Elevated expression of chemokines CCL2, CCL3, CCL4, CCL5, CCL18, and CCL20 (p<0.05) was detected in subcutaneous adipose tissue cells isolated obese patients in comparisons with persons with normal body weight. These findings attest to favorable conditions for enhanced neutrophil migration to the adipose tissue in patients with obesity, which can promote leukocyte infiltration of the suture site after abdominoplasty and serves as additional risk factor for the development of postoperative complications associated with activity of neutrophil-derived proteolytic enzymes.


Subject(s)
Chemokine CCL2/metabolism , Chemokine CCL3/metabolism , Chemokine CCL5/metabolism , Ideal Body Weight , Neutrophils/metabolism , Obesity/metabolism , Subcutaneous Fat/metabolism , Abdominoplasty , Adolescent , Adult , Aged , Body Mass Index , Case-Control Studies , Chemokine CCL2/blood , Chemokine CCL3/blood , Chemokine CCL5/blood , Female , Humans , Ideal Body Weight/physiology , Male , Middle Aged , Obesity/pathology , Obesity/surgery , Subcutaneous Fat/pathology , Young Adult
3.
Article in Russian | MEDLINE | ID: mdl-26411168

ABSTRACT

The article considers experience of application of focus-group technique for both analyzing social psychological conceptions about harm of alcohol consumption during pregnancy and development of prevention programs concerning fetal alcoholic syndrome, an incurable children disease developing because of mother's consumption of alcohol during her pregnancy. The development of preventive program is possible only on the basis the study results allowing getting reliable information about existing in different population groups (first of all in pregnant women, women of childbearing age and medical workers) attitudes concerning alcohol consumption during pregnancy and factors effecting decrease or stopping alcohol consumption by pregnant women as well. The study was carried out involving 7 focus groups: three with physicians (n=23) and four with women (n=23) and their husbands (n=5). The protocols of focus groups were analyzed using software A TLAS-ti 5.0. The most typical attitudes concerning alcohol consumption during pregnancy were established. It was discovered that neither women nor physicians have no essential knowledge about effect of alcohol on fetus and about fetal alcoholic syndrome.


Subject(s)
Alcohol Drinking/adverse effects , Fetal Alcohol Spectrum Disorders/prevention & control , Health Knowledge, Attitudes, Practice , Pregnancy Complications/chemically induced , Adult , Female , Humans , Male , Pregnancy
4.
Patol Fiziol Eksp Ter ; (1): 8-12, 2014.
Article in Russian | MEDLINE | ID: mdl-25051676

ABSTRACT

It was investigated the activity of leukocyte elastase, alpha 1-proteinase inhibitor and C-reactive protein in patients with delayed negativation of serological reactions after a treatment of syphilis. The patients were divided into three subgroups. The first subgroup consisted of patients who did not have the signs of non-specific immune system response. Second subgroup consisted of patients with clear signs of infection process. The patients this subgroup had elevated values of the contents of inflammatory mediators. The remaining patients with questionable results were attributed in the third subgroup. The obtained results can be of great value to determine further treatment strategy in patients with delayed negativation of serological reactions.


Subject(s)
C-Reactive Protein/analysis , Leukocyte Elastase/blood , Syphilis/blood , alpha 1-Antitrypsin/blood , Adult , Case-Control Studies , Humans , Middle Aged , Penicillins/therapeutic use , Serologic Tests , Syphilis/drug therapy , Syphilis/immunology
6.
Klin Lab Diagn ; (6): 26-32, 2011 Jun.
Article in Russian | MEDLINE | ID: mdl-21851007

ABSTRACT

Basic hemogram parameters were analyzed in 835 blood donors (421 men and 414 women) aged 18 to 67 years) on an ADVIA 120 hematology analyzer. Reference ranges were calculated for each parameter in relation to their distribution (as X mean +/- 2alpha for normally distributed data and 2.5-97.5 per thousand for others). The findings suggest that the normal range should be extended for platelets, red blood cells, hemoglobin, and packed cell volume as compared with the earlier unified normal values, which must be taken into account when deciding whether a subject may be allowed to donate blood.


Subject(s)
Blood Cell Count/standards , Blood Donors , Hematocrit/standards , Hemoglobins/standards , Adolescent , Adult , Aged , Female , Humans , Male , Middle Aged , Reference Values
7.
Radiats Biol Radioecol ; 49(2): 207-11, 2009.
Article in Russian | MEDLINE | ID: mdl-19507690

ABSTRACT

The rate of decrease of 137Cs content in hydrobionts from water-bodies of Ukraine that contaminated as result of Chernobyl accident has been calculated. The retrospective analysis of 137Cs content in fish of water-bodies has been transmitted.


Subject(s)
Cesium Radioisotopes/analysis , Chernobyl Nuclear Accident , Fishes/growth & development , Fresh Water/analysis , Radiation Monitoring/methods , Water Pollutants, Radioactive/analysis , Animals , Cesium Radioisotopes/pharmacokinetics , Fishes/metabolism , Ukraine , Water Pollutants, Radioactive/pharmacokinetics
8.
Biomed Khim ; 49(6): 613-22, 2003.
Article in Russian | MEDLINE | ID: mdl-16119090

ABSTRACT

Sera of 80 patients with pyodermias of varius etiology were analysed for interferon-gamma (IF-gamma), antiinterferon autoantibodies (IF-a-AB), corresponding antiidiotypic antibodies (IF-AIAB) and the ratio IF-a-AB/IF-AIAB using ELISA assay. There was no correlation between serum IF-gamma and IF-a-AB. Progression of this disease was characterized by an increase of serum IF-a-AB and a decrease of IF-AIAB. Prolonged duration of this disease also caused the decrease of serum anti IF-a-AB and IF-AIAB. The ratio of IF-a-AB/IF-AIAB increased with severity and duration of this disease.


Subject(s)
Antibodies, Anti-Idiotypic/blood , Autoantibodies/blood , Interferon-gamma/blood , Pyoderma/immunology , Chromatography, Affinity , Humans , Immunoenzyme Techniques , Pyoderma/blood , Pyoderma/etiology , Severity of Illness Index
9.
Genetika ; 32(6): 810-4, 1996 Jun.
Article in Russian | MEDLINE | ID: mdl-8964467

ABSTRACT

It is shown that in vitro infection of human peripheral blood lymphocytes by mycoplasmas (Acholeplasma laid-lawii PG8 and Mycoplasma hominis PG21) leads to multiple chromosome aberrations, predominantly to chromatid gaps and breaks. Oxidative stress in the infected cells is regarded as one of the factors causing chromosome damage.


Subject(s)
Acholeplasma , Chromosome Aberrations , Lymphocytes/microbiology , Mycoplasma Infections/genetics , Mycoplasma , DNA Damage , Humans , Oxidative Stress/genetics
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