[Reactivity of the hemostatic system depending on the model of the behavior and personality characteristics of hypertensive patients under emotional stress]. / Reaktivnost' sistemy gemostaza v zavisimosti ot modeli povedeniia i lichnostnykh osobennostei bol'nykh gipertonicheskoi bolezn'iu v usloviiakh émotsional'noi napriazhennosti.

The results of investigating the effect of emotional stress on the blood coagulation and anticoagulation systems are discussed. It was found that similar conditions of emotional stress cause three types of reactions in different people: functional hypercoagulation, functional hypocoagulation and the absence of any noticeable reaction according to the findings of thrombelastography. The degree of activation of the blood fibrinolytic system depends on the extent of involvement of the patients in interpersonality relationships. Patients with hypertensive disease are characterized by neuroticism, heightened level of anxiety and dependence, and increased emotional aggressiveness. Signs of introversion also prevailed in these patients, which was attributed to the forced formation of new behavior models due to the disease.

Intervesicular phospholipid exchange. An NMR study.

Phospholipid exchange between phosphatidylinositol and phosphatidylcholine ?vesicles has been studied by NMR spectroscopy with use of hydrophilic paramagnetic lanthanide probes (Pr-3+ and Eu-3+ ions). The dependence of the lanthanide induced shifts in the1-H and 31-P NMR spectra on the phospholipid composition of the vesicles could be used for its quantitative evaluation. The method has been proved to be applicable for studying phospholipid exchange stimulated by soluble proteins (postmicrosomal supernatant fraction) from rat liver. Furthermore it has been shown that the phospholipid molecules newly introduced by protein-stimulated exchange are predominantly incorporated into the outermonolayer of the vesicular bilayer membrane. This makes it possible to produce liposomes with asymmetric distribution of the phospholipids across the bilayer.

13-C NMR investigation of phospholipid membranes with the aid of shift reagents.

13-C NMR spectra of sonicated phospholipid dispersions have been investigated with the aid of paramagnetic ions (Pr-3+) as shift reagents. This method permits discrimination between the outer and the inner surfaces of the vesicular membrane and gives a larger variety of signals than 1-H NMR. The lanthanide induced shifts have been found to depend on other ions present and on the lipid composition of the membrane.