ABSTRACT
The efficacy of a new bioantioxidant histochrome is studied in 92 patients (98 eyes). The drug was injected subconjunctivally or parabulbarly in a dose of 0.5 ml 0.02% solution daily, 7-10 injections per course. The clinical effect of histochrome is determined by the localization, duration, and extent of the process. The drug was the most effective in the treatment of intraocular hemorrhages which occurred no more than 7 days before. Electroretinography demonstrated pronounced retinoprotective properties of the drug which improved the electrophysiological parameters in degenerative processes in the retina and optic nerve. Hence, histochrome is characterized by hemoresorption, retinoprotective, and antioxidant properties and is recommended for the treatment of ocular diseases involving metabolic disorders in the retina, vascular membrane, and cornea.
Subject(s)
Antioxidants/therapeutic use , Eye Diseases/drug therapy , Naphthoquinones/therapeutic use , Adolescent , Adult , Aged , Aged, 80 and over , Antioxidants/administration & dosage , Conjunctiva , Female , Humans , Injections , Male , Middle Aged , Naphthoquinones/administration & dosage , Orbit , Treatment OutcomeABSTRACT
Side effects of laser on the posterior corneal epithelium and the retina during intracapsular YAG laser fragmentation of transparent lens fibers in various modes of laser operation were experimentally studied. The pulse power varied from 3 to 7.7 mJ, number of pulses from 55 to 160, length of exposure 10(-8) sec, diameter of the spot in the focus 30 microns. Animal eyes were enucleated on days 3-6 after the procedure. Posterior corneal epithelium was examined in the Hitachi scanning electron microscope, the retina in a light microscope. The findings evidence that injury to the posterior corneal epithelium in intracapsular YAG laser fragmentation of the lens depends mostly on the number of pulses and not on the exposure energy. A safe number of coagulates should not surpass 100, this being quite sufficient for fragmentation of the lens fibers at exposure power of 5-6 mJ. Such operation mode is safe for the retina as well, this recommending it for clinical use.
Subject(s)
Eye/radiation effects , Laser Therapy , Lens, Crystalline/surgery , Animals , Eye/cytology , Laser Therapy/adverse effects , Light Coagulation/adverse effects , Microscopy, Electron, Scanning , RabbitsABSTRACT
The localization of anionic sites on the surface of corneal endothelium was investigated using colloidal nickel. The distribution of the binding sites of colloidal nickel along the cell surface was examined with the scanning electron microscope. The application a special detector of high sensitivity reflection allows to register a composed contrast determined by colloidal nickel. Colloidal nickel is bound with the surface of endothelium in areas of cell junctions only. A 1 X 10(-4)% sol. adrenaline injection into the anterior rabbit eye chamber in vivo changed the distribution of colloidal nickel. These findings demonstrate that colloidal nickel allows to mark the anionic sites on the cell surface for scanning electron microscopic studies.
