[Features of course of pain syndrome in patients with schwannomatosis]. / Osobennosti techeniya bolevogo sindroma u patsientov s shvannomatozami.

OBJECTIVE: To identify the characteristics of pain syndrome in patients with schwannomas depending on genetic predisposition. MATERIAL AND METHODS: The study included 46 patients with peripheral, spinal and intracranial schwannomas, corresponding to the schwannomatosis phenotype according to the 2022 clinical criteria. All patients underwent sequencing of the LZRT1, Nf2 and SMARCB1 and a copy number study in the NF2. RESULTS: The most severe widespread pain was observed in patients with pathogenic LZRT1 variants, while patients with mosaic variants may not even have local tumor-related pain. Patients with SMARCB1variants may have no pain or have localized pain that responds well to surgical treatment. CONCLUSION: Further studies of the molecular features of schwannomatosis and driver mutations in the pathogenesis of pain are necessary to improve the effectiveness of pain therapy in this group of patients. Schwannomatosis is a disease from the group of neurofibromatosis, manifested by the development of multiple schwannomas. Neuropathic pain is one of the main symptoms characteristic of peripheral schwannomas, however, the severity and prevalence of the pain syndrome does not always correlate with the location of the tumors. According to modern concepts, the key factors influencing the characteristics of the pain syndrome are the target gene and the type of pathogenic variant. The most severe widespread pain is observed in patients with pathogenic variants in the LZRT1 gene, while patients with mosaic variants may not even have local pain associated with tumors. Patients with variants in SMARCB1 may have no pain or localized pain that responds well to surgical treatment.

[Clinical aspects of the service of acute postoperative pain treatment].

PURPOSE OF THE STUDY: To analyze work results of the service of acute postoperative pain treatment in the orthopedic hospital. DISIGN: Single-center retrospective observational cohort study. PATIENTS AND METHODS: We studied 1343 patients underwent total hip arthroplasty (64%), total knee arthroplasty (33%), and revision surgeries (3%). The average age of the patients was 59 ± 12 years, and body mass index was 30 ± 6. All patients received triple pain therapy in postoperative period which included pracetamol, NSA IDs or specific ciclooxygenase-2 inhibitor and epidural analgesia or peripheral blockade and patient controlled intravenous or epidural analgesia. The pain intensity was recorded four times a day--every 6 hours during 2 days after surgeries. RESULTS: All patients received oral or intravenous analgesics. 90% of patients received prolonged epidural infusion, 10%--prolonged block of the femoral nerve after total knee arthroplasty. Patient controlled analgesia was used in 6-10% of patients. Average pain intensity was 20 mm according to VAS. Pain intensity over 40 mm was observed in 8-13% of patients at 1st day after surgery and in 2-15% at 2nd day after surgery. Deep vein thrombosis was found in 5.7% of patients, and bleeding in 0.2%. Length of stay in a hospital after total hip arthroplasty and total knee arthroplasty was 6 ± 2 days and 10 ± 8 days after revision surgeries. CONCLUSIONS: The work of the service of acute postoperative pain treatment in the orthopedic hospital, the use of multimodal treatment protocols and mandatory clinical monitoring provide proper control of postoperative pain, patient satisfaction, and allow to accelerate early recovery and reduce the time of postoperative hospital stay after total replacement of large joints.

[Coxibs for postoperative analgesia].

Coxibs can be regarded as an effective way of postoperative pain treatment with proven analgesic and opioid-saving effects. When comparing the opioid-saving effect after the large surgical interventions, COX-2 inhibitors are not inferior to NSAIDs and surpass paracetamol. The combination of coxibs and opiate receptors antagonists, as well as epidural analgesia is effective in the frames of multimodal analgesia. The reasonability of coxibs and paracetamol combination is questionable. In patients at risk of gastrointestinal complications development, but with none cardiovascular risk, COX-2 inhibitors are more safe, than the combination of NSAIDs and proton pump inhibitors. Due to no cross-reactivity with aspirin and NSAIDs, coxibs can be recommended to patients with aspirin asthma and related diseases. Specific COX-2 inhibitors prescription is able to inhibit comissure formation after laparotomy, suppressing blood vessels proliferation. It is assumed that the COX-2 inhibitors may inhibit vascular endothelial growth factor of the tumor and so inhibit angiogenesis of solitary tumors and metastases, without affecting the normal endothelium. Thus, today coxibs are not inferior in eficiency to certain opioid analgesics and have improved safety profile compared with traditional NSAIDs. These qualities allow to consider them as a group of non-opioid analgesics for postoperative analgesia.

[Immunity and cytokine status after surgeries on the large intestine].

The aim of our study was to examine the effect of individual schemes of multimodal analgesia on indicators of immunity and inflammation markers after operations on the colon. Patients of group 1 (n=15) received paracetamol, lornoxicam and epidural ropivacaine, 2nd group of patients (n=15)-paracetamol, epidural ropivacaine and tramadol. Comparison group (n=10) patients underwent patient controlled analgesia by promedol. Before surgery, 1st and 3rd days after surgery we examined the contents of cytokines in plasma: interleukin 12p70, interleukin 10, interleukin 6, and TNF. Before surgery and at 5-7 days after surgery indicators of cellular, fagocytal and humoral immunity were monitored. Before surgery patients with colorectal cancer revealed changes in the indices of different components of immunity, as well as an increase in pro-and anti-inflammatory cytokines compared with healthy donors. Multimodal analgesia in patients after operations on the colon is not accompanied by changes in plasma concentrations of cytokines and parameters of immune status in comparison with monoanalgesia by promedol.

[Spectrophometric analysis of volatile compounds in microorganisms].

A simple modification of a spectrophometric method was proposed for the rapid detection of microorganisms based on their ability either to excrete or to absorb volatile compounds. The method provides the possibility of contactless control for bacterial growth at a concentration above 10(7) cells/ml. In addition, the method allows discriminating mutants of the fungus Neurospora crassa defective in the nitrogen metabolism from the wild type strains. It is likely that nitrite reductase and nitrate reductase enzymes regulated by the nit-2 and nit-6 genes are involved in formation of the water soluble volatile compounds of this organism.

[Comparison of analgesics using two devices for the patient-controlled analgesia].

INTRODUCTION: A pilot unicenter blind placebo-controlled study comparing different analgesics was conducted in parallel groups, by concurrently using two patient-controlled analgesia (PCA) apparatuses in one patient, was conducted Subjects and methods. The study included patients after laparotomy. Group I patients (n=22) were proposed 2 PCA apparati, one of which contained trimeperidine solution and the other did placebo. Group 2 patients (n=17) were proposed 2 PCA apparatuses, one of which contained trimeperidine solution and the other did lornoxycam. PCA was adjusted in the same manner in both apparati. The patients were asked to determine which analgesic is best in providing 24-hour analgesia. RESULTS: There were no differences in the intensity of pain between the groups. The latter did not differ in the number of adverse reactions. Comparison showed no significant differences in the use of trimeperidine between Groups 1 and 2 patients. Among Group 1 patients, 61% considered trimeperidine as the best analgesic and 22% did placebo; 17% called none. Group 2 patients chose trimeperidine in 41% of cases and lornoxycam in 41%, none was called in 18%. CONCLUSION: Comparative assessment of analgesics, by employing 2 PCA apparatuses in one patient, allows evaluation of the efficacy of analgesics added to the conventional methods.

[Investigation of electrophysical properties of Listeria monocytogenes cells during the interaction with monoclonal antibodies].

An electrooptical approach was used in studies of Listeria monocytogenes-antibody binding. An electrooptical analyzer, which has been developed at the State Research Center for Applied Microbiology (Obolensk, Russia), was used as a basic instrument for electrooptical measurements. The analyzer consists of the following modules: a sample preparation module, a mixer, an AC field generator, an EO-flow cell, a microcontroller for transfer of liquid, a thermal system, an operator interface, and an image processor. The sample preparation module includes a unit for an automatic filter changing device and a hydraulic system. Since the AC electrokinetic effects depend on the dielectric properties of bioparticles, their composition, morphology, phenotype, the medium, and the frequency of applied electrical field, the electroorientational spectra were used for discrimination of different types of bacteria, a given type being "controlled" (and identified) by the selective choice of binding agents (antibodies). The measurements were performed using a discrete set of frequencies of the orienting electric field (10, 100, 250, and 500 kHz). During biospecific interactions, an antibody is bound to the microorganism, causing a change in the dielectric properties of the microorganism-antibody complex, and the electrooptic signal reaches its maximum at 100-200 kHz. It was shown that the biospecific interactions of Listeria monocytogenes cells with anti-Listeria antibody in the presence of E. coli K-12, and A. brasilense sp7 significantly change the electrooptical signals. Thus, the determination of the presence of particular bacteria within a mixed sample may be achieved by selection and matching of antibodies specific to individual bacterium types and by comparing the spectra of bacterium in the presence and in the absence of specific binding agent (antibody).

[Bacterial motion in porous media]. / Dvizhenie bakterii v poristykh sredakh.

The motion of chemotactically different Escherichia coli C600, cheB287, and AW405 cells was studied using a column packed with silica gel. The model chemotaxis of bacteria in porous media seems to be adequate to natural bacterial chemotaxis in soils. The porous structure of silica gel prevents interfering convective flows. Silica gel columns make it possible to separate bacterial cells differing in motility and chemotaxis. Relevant physical phenomena are discussed. The concept of fast and slow chemotaxis is considered.

Electrophysical monitoring of culture process of recombinant Escherichia coli strains.

A novel method for monitoring the cell culture process has been developed. The method is based on the measurements of electro-optical characteristics of cell suspension, calculation of cell structure parameters, and the relationship between accumulation of proteins and change of these parameters' employment. Application of the method for the monitoring of a culture process of a recombinant strain is considered. The process of growth of recombinant strains cannot be sufficiently predicted and the direct measurement of cell culture parameters is unlikely to be the most efficient way of solving the problem.Escherichia coli plasmid-free and recombinant strains synthesizing the fusion protein consisting of tumor necrosis factor-alpha (TNF) and thymosin-alpha(1) (T) were studied. It was found that cytoplasmic electroconductivity of the strains investigated increased during the culture process. The accumulation of insoluble recombinant pThy-315-encoded hybrid protein TNF(SINGLEBOND)T in cells resulted in a decrease of the membrane dielectric permeability. To determine variations of membrane dielectric permeability the amount of insoluble recombinant protein TNF(SINGLEBOND)T in the bacterial cells should be calculated.

[Determination, by the electro-optical method, of the number of undamaged bacterial cells after extreme exposures]. / Opredelenie élektroopticheskim metodom chisla nepovrezhdennykh bakterial'nykh kletok posle ékstremal'nykh vozdeistvii.

The results of electro-optical measurements can be used to determine rapidly how the number of intact cells decreases before and after the action of certain extreme factors. The experiments were conducted with suspensions of Escherichia coli and Bacillus thuringiensis cells subjected to the action of heat, toluene, ethanol, and freezing. Electro-optical measurements can make it possible to assay the relative number of cells with both lethal and sublethal damages.

[Mechanism of catabolite repression in Escherichia coli bacteria: interaction between transport proteins and adenylate cyclase]. / Mekhanizm katabolitnoi repressii u bakterii E. coli: vzaimodeistvie mezhdu transportnymi belkami i adenilattsiklazoi.

The mechanism of catabolite repression caused by sugar transported via the phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS) and stipulated by the decrease of the adenylate cyclase activity was studied. It was demonstrated that the sensitivity of the adenylate cyclase and beta-galactosidase synthesis to methyl-L-D-glucoside (MeGlc) or sorbitol is correlated with the content and activity of glucose (EIIGlc) or mannitol enzyme II of the PTS, correspondingly. Under anaerobic conditions the cells become insensitive to catabolic repression caused by MeGlc and the adenylate cyclase activity does not decrease in the presence of the sugar despite the increased rate of MeGlc transport. The adenylate cyclase activity of the mutant with the Tn5 transposone inserted into the ptsG gene does not change in the presence of MeGlc, while the activity of adenylate cyclase and the differential rate of beta-galactosidase synthesis increase in these bacteria. The data obtained confirm the hypothesis on the "catabolite signal" which is generated when the substrate binds to its transporter, i. e. adenylate cyclase reacts to the conformational changes in the transporter being complexed with it. The strength of this complex depends on the affinity of adenylate cyclase for the transporter and on the value of the membrane potential, delta mu H+ A model is proposed, which explains the necessity of factor IIIGlc for EIIGlc binding to adenylate cyclase.