Single-nucleotide polymorphism (rs11204981) in filaggrin gene and its functional significance for asthma among children with eczema.

The aim of this study was to determine whether SNP in filaggrin gene and expression of filaggrin mRNA in buccal epithelium are associated with childhood eczema and with the phenotype of childhood eczema combined with asthma. Genotyping for FLG (rs11204981) was performed in the following populations: patients with asthma (n = 99); ages 5-18 years (8 ± 2.1), and control group (n = 98); ages 5­18 years (12 ± 2.1) by using Real-time PCR. Level of mRNA expression was estimated by using reverse transcription and following real-time PCR. It was found out that 5.05 % of patients and 2.02 % of control group had minor allele (AA; P>0.05), 27.27 % and 36.36 % of patients and control group, respectively, had heterozygous allele (GA; P>0.05) and 67.68 % and 61.62 % had major allele (GG) (P>0.05). Variants with the AA-genotype of the FLG rs11204981 were found to be 2.5 times more frequently among patients than in control group. We also found out that the level of mRNA FLG expression in GG-genotype is 22.8 ± 11.67 (P>0.05 compared to AA-genotype), 92.95 ± 35.3 in GA genotype (P<0.05compared to GG-genotype) and 21.8 ± 13.4 in AA genotype (P>0.05 compared to GA-genotype). Thus, heterozygous variant has significantly higher expression of filaggrin in buccal epithelium. We suggest that SNP in FLG (rs11204981) may serve as an important predictive marker for the combined eczema plus asthma phenotype, and that the highest level of expression in heterozygous may have a protective role in developing allergy phenotype. Key words: snp; filaggrin; asthma; paediatrics.

[PUBLIC ADMINISTRATION OF PERSONNEL POLICY IN REFORMING OF UKRAINIAN HEALTH CARE SYSTEM USING THE EXAMPLE OF DERMATOVENEREOLOGICAL SERVICE].

The health care system, its modernization and optimization are among the most important functions of the modern Ukrainian state. The main goal of the reforms in the field of healthcare is to improve the health of the population, equal and fair access for all to health services of adequate quality. Important place in the health sector reform belongs to optimizing the structure and function of dermatovenereological service. The aim of this work is to address the issue of human resources management of dermatovenereological services during health sector reform in Ukraine, taking into account the real possibility of disengagement dermatovenereological providing care between providers of primary medical care level (general practitioners) and providers of secondary (specialized) and tertiary (high-specialized) medical care (dermatovenerologists and pediatrician dermatovenerologists), and coordinating interaction between these levels. During research has been found, that the major problems of human resources of dermatovenereological service are insufficient staffing and provision of health-care providers;,growth in the number of health workers of retirement age; sectoral and regional disparity of staffing; the problem of improving the skills of medical personnel; regulatory support personnel policy areas and create incentives for staff motivation; problems of rational use of human resources for health care; problems of personnel training for dermatovenereological service. Currently reforming health sector should primarily serve the needs of the population in a fairly effective medical care at all levels, to ensure that there must be sufficient qualitatively trained and motivated health workers. To achieve this goal directed overall work of the Ministry of Health of Uktaine, the National Academy of Medical Sciences of Ukraine, medical universities, regional health authorities, professional medical associations. Therefore Ukrainian dermatovenereological care, in particular fixed, needs a deep and objective medical and social audit. A necessary condition for the harmonious development of dermatovenereological service is adequate staffing to ensure it to reflect changes in the structure of the provision of the assistance at various levels, as well as their effective coordination throughout the natient's medical route.