Reduction of scum accumulation through the addition of low-cost enzymatic extract in the feeding of high-rate anaerobic reactor.

This work evaluates the reduction of scum accumulation on the top surface of upflow anaerobic sludge blanket (UASB) reactors by the addition of hydrolytic enzymes in their feed. For over 1 year, two UASB reactors of 1.4 L were maintained at 30 °C and continuously fed with synthetic domestic wastewater (containing 150 mg/L of soybean oil) under a hydraulic retention time of 10 h. The Control reactor was only fed with synthetic wastewater. Beginning at the 226th day of operation, low-cost hydrolytic enzymes (obtained by solid-state fermentation of Aspergillus terreus, a fungus isolated from a primary sewage sludge) were added into the feed of the other reactor (Test) for a lipase activity of 24 U/L, considerably reducing the formation of scum. In the Test reactor, the scum showed oil and grease (O&G) concentration between 0.8 and 1.3 g/L and an accumulation rate of 20 to 27 mg O&G/d. In the Control reactor, the scum had values twice as high (1.5-2.5 g/L and 34-51 mg O&G/d, respectively) and there were more operational problems. During the entire period of operation, both reactors presented high chemical oxygen demand removal (>80%), with no loss of effluent quality due to the addition of the enzymes.

Co-digestion of sewage sludge with crude or pretreated glycerol to increase biogas production.

Anaerobic co-digestion of sewage sludge and glycerol from the biodiesel industry was evaluated in three experimental stages. In the first step, the addition of higher proportions of crude glycerol (5-20% v/v) to the sludge was evaluated, and the results showed a marked decrease in pH and inhibition of methane production. In the second step, co-digestion of sludge with either a lower proportion (1% v/v) of crude glycerol or glycerol pretreated to remove salinity resulted in volatile acid accumulation and low methane production. The accumulation of volatile acids due to the rapid degradation of glycerol in the mixture was more detrimental to methanogenesis than the salinity of the crude glycerol. In the third step, much lower amounts of crude glycerol were added to the sludge (0.3, 0.5, 0.7% v/v), resulting in buffering of the reaction medium and higher methane production than in the control (pure sludge). The best condition for co-digestion was with the addition of 0.5% (v/v) crude glycerol to the sewage sludge, which equals 0.6 g glycerol/g volatile solids applied. Under this condition, the specific methane production (mL CH4/g volatile solids applied) was 1.7 times higher than in the control.