Traditional and biodegradable plastics host distinct and potentially more hazardous microbes when compared to both natural materials and planktonic community.

Microplastic particles are persistent micropollutants that provide a substrate for the growth of bacterial biofilms, posing a threat to the environment. This study explores the changes in commercially available food containers made of conventional (polypropylene PP, polyethylene terephthalate PET), innovative biodegradable (Mater-Bi) and natural (wood and cellulose) materials, when introduced in the surface waters of Lake Maggiore for 43 days. Spectral changes revealed by FT-IR spectroscopy in PET and Mater-Bi, and changes in thermal properties of all human-made material tested indicated a degradation process occurred during environmental exposure. Despite similar bacterial richness, biofilms on PET, PP, and Mater-Bi differed from natural material biofilms and the planktonic community. Human-made material communities showed a higher proportion of potential pathogens, with PET and PP also exhibiting increased abundances of antibiotic resistance genes. Overall, these findings stress the need for dedicated strategies to curb the spread of human-made polymers in freshwaters, including innovative materials that, due to their biodegradable properties, might be perceived less hazardous for the environment.

Limited contribution of hatchery-produced individuals to the sustainment of wild marble trout (Salmo marmoratus Cuvier, 1829) in an Alpine basin.

Fish stocking constitutes a common management practice in freshwaters all over the world, to enhance fisheries or to support threatened fish populations. Pervasive detrimental effects may affect the real effectiveness of stocking programs. However, studies assessing the real impacts and relative contribution of stocked trout in wild populations are surprisingly few. The marble trout, Salmo marmoratus (Cuvier 1829), is a critically endangered sub-endemic salmonid in Northern Italy, and an iconic species for recreational fishing and conservation, also representing an emblematic case of species negatively affected by restocking. For instance, marble trout inhabiting the Toce River, the second largest tributary of Lake Maggiore, has been stocked with different hatchery congener trout belonging to the Salmo trutta complex (putative marble trout, Atlantic trout Salmo trutta Linnaeus 1758 and putative Mediterranean trout Salmo ghigii Pomini 1941) over the last decades. Using mitochondrial (D-loop) and nuclear (12 microsatellites and LDH-C1*) markers, we characterised the genetic variability and gene flow among the wild and hatchery individuals of marble trout of this basin, to investigate the effectiveness of stocking activities on the native residual population. Despite extensive hybridization of marble trout with non-native brown trout stocks was shown, the presence of individuals belonging to pure native stock has been detected as well. However, concerns could be advanced regarding its future persistence, due to climatic and hydraulic instabilities or loss of environmental heterogeneity. Moreover, despite ongoing yearly massive stocking activities, a negligible contribution of reared putative marble trout in the wild sample has been documented, suggesting that natural recruitment represents the greatest source of this wild population sustainment. Important adaptive differences between wild and domestic trout are present, likely due to the deleterious long-term effects of the close breeding system of hatcheries. Finally, possible implications for stocking management improvement have been discussed.

Size-age population structure of an endangered and anthropogenically introgressed northern Adriatic population of marble trout (Salmo marmoratus Cuv.): insights for its conservation and sustainable exploitation.

Salmonid species are main actors in the Italian socio-ecological landscape of inland fisheries. We present novel data on the size-age structure of one of the remnant Italian populations of the critically endangered marble trout Salmo marmoratus, which co-occurs with other stocked non-native salmonids in a large glacial river of the Lake Maggiore basin (Northern Italy-Southern Switzerland). Like other Italian native trout populations, the Toce River marble trout population is affected by anthropogenic introgression with the non-native brown trout S. trutta. Our sample includes 579 individuals, mainly collected in the Toce River main channel. We estimated the length-weight relationship, described the population size-age structure, estimated the age-specific growth trajectories, and fit an exponential mortality model. A subset of the sample was also used to measure numerical and biomass density. The estimated asymptotic maximum length is ~105 cm total length (TL). Mean length at first maturity is ~55 cm TL, and mean length at maximum yield per recruit is ~68 cm TL. Approximately 45-70% of the population are estimated to die annually, along with a fishing annual mortality of ~37%, with an exploitation ratio of ~0.5. The frequency distribution of length classes in a sample collected by angling shows that ~80% of the individuals that could be retained according to the current recreational fishing regulations likely never reproduced, and large fish disproportionally contributing to recruitment are fished and retained. We identify possible overfishing risks posed by present regulations, and propose updated harvest-slot length limits to mitigate such risks. More detailed and long-term datasets on this system are needed to more specifically inform the fishery management and monitor the effects of any change in the management strategy on the size-age structure of the marble trout population of the Toce River.

What's the effectiveness of stocking actions in small creeks? The role of water discharge behind hatchery trout downstream movement.

Fish stocking to enhance freshwater fisheries or to improve the conservation status of endangered fish species is a common practice in many countries. Little is known, however, of the effectiveness of these practices in spite of the high efforts and investments required. The movement of subadult/adult hatchery-released brown trout Salmo trutta L. was studied by passive telemetry in a small tributary of Lake Lugano (i.e., Laveggio Creek, Canton Ticino, Switzerland). Hatchery fish, together with some resident wild individuals sampled during electrofishing surveys, were tagged with Passive Integrated Transponders (PIT) tags. Hatchery fish were released upstream and downstream a submersible monitoring antenna, which was anchored to the streambed in a pass-over orientation. The number of hatchery fish detected daily by the antenna (divided between fish released upstream and downstream the antenna) was analyzed in relation to the daily water discharge, to search for similar patterns in their fluctuation over time. Only the movement of fish released upstream the antenna displayed a significant relationship with water discharge, with the highest number of fish detected during periods of high-water flow, occurring after heavy rains. High-water discharge events had a significant role in hatchery trout downstream movement in our study site, likely acting as a driver for the downstream migration to Lake Lugano. Such events contributed to the poor effectiveness of stocking actions in this small tributary, providing further evidence against stocking strategies based on subadult/adult fish.

Can size distributions of European lake fish communities be predicted by trophic positions of their fish species?

An organism's body size plays an important role in ecological interactions such as predator-prey relationships. As predators are typically larger than their prey, this often leads to a strong positive relationship between body size and trophic position in aquatic ecosystems. The distribution of body sizes in a community can thus be an indicator of the strengths of predator-prey interactions. The aim of this study was to gain more insight into the relationship between fish body size distribution and trophic position in a wide range of European lakes. We used quantile regression to examine the relationship between fish species' trophic position and their log-transformed maximum body mass for 48 fish species found in 235 European lakes. Subsequently, we examined whether the slopes of the continuous community size distributions, estimated by maximum likelihood, were predicted by trophic position, predator-prey mass ratio (PPMR), or abundance (number per unit effort) of fish communities in these lakes. We found a positive linear relationship between species' maximum body mass and average trophic position in fishes only for the 75% quantile, contrasting our expectation that species' trophic position systematically increases with maximum body mass for fish species in European lakes. Consequently, the size spectrum slope was not related to the average community trophic position, but there were negative effects of community PPMR and total fish abundance on the size spectrum slope. We conclude that predator-prey interactions likely do not contribute strongly to shaping community size distributions in these lakes.

The Italian Alpine and Subalpine trouts: Taxonomy, Evolution, and Conservation.

During the last 150 years, the trout-culture industry focused on enhancing trout populations by stocking, in response to the growing anglers' demand and the habitat degradation associated to the rapid urbanization and hydropower development. The industrialized north of Italy, home to the Italian Alpine and subalpine trout populations, is the source of most of the revenues of the national trout-culture industry. Its rapid growth, and the massive introduction of non-native interfertile trouts eroded the genetic diversity of native lineages, leading to harsh confrontations between scientists, institutions, and sportfishing associations. We review here the state of the art of the taxonomy and distribution of the northern Italian native trouts, presenting both scientific results and historical documentation. We think the only native trouts in this region are Salmo marmoratus, widespread in this region, plus small and fragmented populations of S. ghigii, present only in the South-western Alps. We strongly recommend the interruption of stocking of domesticated interfertile non-native trouts in this area, and recommend the adoption of Evolutionary Significant Units for salmonid fishery management. We further propose future research directions for a sustainable approach to the conservation and ecosystem management of the fishery resources and inland waters of northern Italy.

Fish communities in Italian sub-alpine lakes: Non-native species and anthropogenic pressures increase community dissimilarities.

In European lakes, anthropogenic pressures have increased significantly since the 1950s, facilitating colonisation by non-native species and increasing the potential for further invasions. Here, we determined the effects of anthropogenic pressures (i.e., habitat alterations and introduction of non-native species) on the fish communities of Italian sub-alpine lakes. We hypothesised that established non-native species would have more competitive traits against anthropogenic stressors, such as habitat alteration, than native species. Thus, we expected that non-native species would dominate lake communities and reduce native species occurrence and abundance depending on the degree of anthropogenic alterations. Overall, we predicted that the increase in anthropogenic pressures after the 1950s had led to homogenisation of the fish communities of the lakes in the region. We tested these hypotheses using data on 15 sub-alpine lakes, covering a broad geographical and morphological gradient, and compared the 2007-2014 fish community composition (sampled according to the CEN protocol plus point-abundance electrofishing) with variables of lake habitat and anthropogenic pressures (based on the Lake Habitat Survey, a method to evaluate the hydromorphological conditions of lakes according to the European Water Framework Directive) and fish communities before 1950, the latter based on bibliographic information. Following our hypothesis, non-native species showed higher prevalence of traits that increase their competitiveness against anthropogenic alterations (e.g., tolerance to pollution). In addition to lake morphology, the community composition of non-native fish determined as abundance (NPUE) and biomass (BPUE) was positively related to anthropogenic pressures. Since the 1950s, 19 non-native species have colonised the Italian sub-alpine lakes, and the occurrence of native species has decreased by ~27%. However, contrary to our expectation, these changes have increased the ß-diversity of the fish communities in the lakes.

Microplastic retention in small and medium municipal wastewater treatment plants and the role of the disinfection.

Wastewater treatment plants (WWTPs) efficiently retain microplastic particles (MPs) generated within urban areas. Among the wastewater treatment steps, disinfection has not been characterized for its potential MPs retention activity, although it has been reported that processes used to abate the bacterial load could also affect MPs concentration. For this reason, we evaluated the MPs concentration across the overall wastewater treatment process and before and after the disinfection step in four small/medium WWTPs located in the north of Italy. Most of the MPs found in the samples were fibers or fragments, smaller than 500 µm, mainly composed of polyethylene, polypropylene, or polyethylene terephthalate. The retention efficiency at the outlets was higher than 94% in all the plants analyzed. More interestingly, the disinfection treatments adopted by the different WWTPs reduced MPs concentration from a minimum of 9.1% (UV treatment) to a maximum of 67.6% (chlorination), promoting a further increase of the overall retention efficiency of the WWTPs from 0.4 to 0.7%. Quantitatively, the disinfection contributes to the MPs reduction in the outlets by retaining 0.5-6.7 million MPs per day, in WWTPs that discharge 2.7-12 million MPs per day. The results of the present work underline the importance of a careful choice of the steps that constitute the wastewater treatment, including disinfection, in order to minimize MPs discharge into the natural ecosystems.

European fish-based assessment reveals high diversity of systems for determining ecological status of lakes.

Triggered by the adoption of the Water Framework Directive, a variety of fish-based systems were developed throughout Europe to assess the ecological status of lakes. This paper provides a comprehensive overview of all existing systems and summarizes sampling methods, fish community traits (metrics) and the relevant anthropogenic pressures assessed by them. Twenty-one European countries developed fish-based assessment systems. Three countries each developed two distinct systems to approach different ecoregions, either to use different data, or to assess different lake types leading to a total number of 24 systems. The most common approach for the setting of reference conditions, used in seventeen systems, was the utilisation of fish communities in comparably undisturbed natural lakes as reference. Eleven used expert judgment, nine historical data and eight modelled relationships. Fourteen systems combined at least two approaches. The most common fish sampling method was a standardized fishing procedure with multimesh-gillnets. Many countries applied combinations of fishing methods, e.g. non-standard gillnets, fyke nets and electrofishing. Altogether 177 metrics were used for index development and each system combined 2-13 metrics. The most common ones were total standardized catches of number and biomass, relative abundance of Perca fluviatilis, Rutilus rutilus, and Abramis brama, feeding preferences, sensitive species, and non-natives. The pressure-response-relationships for these metrics were supported with both correlations established during system development and scientific publications. However, the metrics and their combinations were highly diverse and no metric was applied universally. Our analysis reveals that most fish-based assessment systems address multiple pressures (eutrophication, hydromorphological alterations, fishery pressure and occurrence of non-natives), whilst few are pressure-specific, tackling only eutrophication or acidification. We argue that the value of fish-based systems for lakes lies in their capacity to capture the effect of many different pressures and their interactions which is lacking for most assessment systems based on other biota.

Microplastics pollution in the terrestrial environments: Poorly known diffuse sources and implications for plants.

Research on microplastics (MPs) in the terrestrial environment is currently at a still embryonal stage. The current knowledge concerning poorly known diffuse sources of MPs pollution in terrestrial ecosystems have been considered in this work. In addition, a particular focus on the presence, mechanism of absorption and effects of MPs in plants has also been provided. Research concerning microplastics in urban areas and their intake by Tyre and Road Wear Particulates (TWRP) demonstrated a high contribution of this plastic debris to microplastic pollution, although quantification of these inputs is challenging to assess because studies are still very few. Around 50% of particles are expected to remain in the roadside soil, while the rest is transported away by the runoff with high concentrations of TRWP with a size ranging between 0.02 and 0.1 mm. Natural and anthropic environments like temporary ponds, stormwater retention ponds and small waterbodies were considered sensitive connecting ecosystems rich in biodiversity between terrestrial and aquatic environments. Even if studies are not yet exhaustive and just eight studies were currently published concerning these ecosystems, considerable values of MPs were already observed both in the sediment and water phase of ponds. Although still poorly explored, agricultural environments were already demonstrated to hide a significant number of microplastics linked mainly to agricultural activities and practices (e.g. mulch, sewage and compost fertilisation). However, the microplastics transportation processes into the soil are still understudied, and a few works are available. Microplastics and primarily nanoplastics presence was also observed in common edible plants (fruit and vegetables) with alarming Estimated Daily Intakes ranging from 2.96 × 1004 to 4.62 × 1005 (p kg-1 day-1) for adults depending on species. In addition, adverse effects on plants growth, photosynthetic activity, antioxidant system and nutritional values of several common fruits and vegetables were also demonstrated by several studies.

Microplastic pollution in perch (Perca fluviatilis, Linnaeus 1758) from Italian south-alpine lakes.

Microplastic particles (MPs) contamination of aquatic environments has raised a growing concern in recent decades because of their numerous potential toxicological effects. Although fish are among the most studied aquatic organisms, reports on MPs ingestion in freshwater environments are still scarce. Thus, there is still much to study to understand the uptake mechanisms, their potential accumulation among the food webs and their ecotoxicological effects. Here, MPs presence in the digestive system of one of the most widespread and commercially exploited freshwater fish, the perch (Perca fluviatilis, Linnaeus 1758), was investigated in four different south-alpine lakes, to assess the extent of ingestion and evaluate its relation to the body health condition. A total of 80 perch specimen have been sampled from the Italian lakes Como, Garda, Maggiore and Orta. Microplastic particles occurred in 86% of the analysed specimens, with average values ranging from 1.24 ± 1.04 MPs fish-1 in L. Como to 5.59 ± 2.61 MPs fish-1 in L. Garda. The isolated particles were mainly fragments, except in L. Como where films were more abundant. The most common polymers were polyethylene, polyethylene terephthalate, polyamide, and polycarbonate, although a high degree of degradation was found in 43% of synthetic particles, not allowing their recognition up to a single polymer. Despite the high number of ingested MPs, fish health (evaluated by means of Fulton's body condition and hepatosomatic index) was not affected. Instead, fullness index showed an inverse linear relationship with the number of ingested particles, which suggests that also in perch MPs presence could interfere with feeding activity, as already described for other taxa.

Contribution of microplastic particles to the spread of resistances and pathogenic bacteria in treated wastewaters.

Microplastic Particles (MPs) are ubiquitous pollutants widely found in aquatic ecosystems. Although MPs are mostly retained in wastewater treatment plants (WWTPs), a high number of MPs reaches the open waters potentially contributing to the spread of pathogenic bacteria and antibiotic resistance genes in the environment. Nowadays, a limited number of studies have focused on the role of MPs as carriers of potentially pathogenic and antibiotic resistant bacteria in WWTPs. Thus, an investigation on the community composition (by 16S rRNA gene amplicon sequencing) and the abundance of antibiotic and metal resistance genes (by qPCR) of the biofilm on MPs (the plastisphere) and of planktonic bacteria in treated (pre- and post-disinfection) wastewaters was performed. MPs resulted to be very similar in terms of type, color, size, and chemical composition, before and after the disinfection. The bacterial community on MPs differed from the planktonic community in terms of richness, composition, and structure of the community network. Potentially pathogenic bacteria generally showed higher abundances in treated wastewater than in the biofilm on MPs. Furthermore, among the tested resistance genes, only sul2 (a common resistance gene against sulfonamides) resulted to be more abundant in the plastisphere than in the planktonic bacterial community. Our results suggest that the wastewater plastisphere could promote the spread of pathogenic bacteria and resistance genes in aquatic environment although with a relatively lower contribution than the wastewater planktonic bacterial community.

Helminth communities of endemic cyprinoids of the Apennine Peninsula, with remarks on ectoparasitic monogeneans, and a description of four new Dactylogyrus Diesing, 1850 species.

The fauna of the Apennine Peninsula is, in comparison to other southern European peninsulas, relatively species-poor regarding the number of endemic cyprinoid species. Nonetheless, the recent introduction of non-native species has significantly increased the total number of freshwater species in this region. Such invasive species may represent a threat to the native fauna, associated among other things with the introduction of non-native parasites with their original hosts.In the present study, we investigated endemic cyprinoid species for the presence of helminth parasites. A total of 36 ectoparasitic monogenean species and five endoparasitic helminth species were collected from ten cyprinoid species in five localities in northern Italy. Out of 20 Dactylogyrus species (gill monogeneans specific to cyprinoids), four were identified as new to science and herein described: Dactylogyrus opertus n. sp. and Dactylogyrus sagittarius n. sp. from Telestes muticellus, Dactylogyrus conchatus n. sp. from T. muticellus and Protochondrostoma genei, and Dactylogyrus globulatus n. sp. from Chondrostoma soetta. All new Dactylogyrus species appear to be endemic to the Apennine Peninsula; however, they share a common evolutionary history with the endemic Dactylogyrus parasitizing cyprinoids of the Balkans. This common origin of cyprinoid-specific parasites supports a historical connection between these two (currently separated) geographical regions.

Climate warming restructures an aquatic food web over 28 years.

Climate warming can restructure lake food webs if trophic levels differ in their thermal responses, but evidence for these changes and their underlying mechanisms remain scarce in nature. Here we document how warming lake temperatures by up to 2°C, rather than changes in trophic state or fishing effort, have restructured the pelagic food web of a large European lake (Lake Maggiore, Italy). Our approach exploited abundance and biomass data collected weekly to yearly across five trophic levels from 1981 to 2008. Temperature generally had stronger effects on taxa than changes in fish predation or trophic state mediated through primary productivity. Consequently, we found that, as the lake warmed, the food web shifted in numerical abundance towards predators occupying middle trophic positions. Of these taxa, the spiny water flea (Bythotrephes longimanus) most prospered. Bythotrephes strongly limited abundances of the keystone grazer Daphnia, strengthening top-down structuring of the food web. Warmer temperatures partly restructured the food web by advancing peak Bythotrephes densities by approximately 60 days and extending periods of positive population growth by three times. Nonetheless, our results suggested that advances in the timing and size of peak Bythotrephes densities could not outpace changes in the timing and size of peak densities in their Daphnia prey. Our results provide rare evidence from nature as to how long-term warming can favour higher trophic levels, with the potential to strengthen top-down control of food webs.

Effects of climate and land-use changes on fish catches across lakes at a global scale.

Globally, our knowledge on lake fisheries is still limited despite their importance to food security and livelihoods. Here we show that fish catches can respond either positively or negatively to climate and land-use changes, by analyzing time-series data (1970-2014) for 31 lakes across five continents. We find that effects of a climate or land-use driver (e.g., air temperature) on lake environment could be relatively consistent in directions, but consequential changes in a lake-environmental factor (e.g., water temperature) could result in either increases or decreases in fish catch in a given lake. A subsequent correlation analysis indicates that reductions in fish catch was less likely to occur in response to potential climate and land-use changes if a lake is located in a region with greater access to clean water. This finding suggests that adequate investments for water-quality protection and water-use efficiency can provide additional benefits to lake fisheries and food security.

Plastic sources: A survey across scientific and grey literature for their inventory and relative contribution to microplastics pollution in natural environments, with an emphasis on surface water.

Plastic debris are at present recognized as an emerging potential threat for natural environments, wildlife and humans. In the past years an increasing attention has been addressed to investigate the presence and concentration of plastic debris in the ecosystems, including surface waters. Scientific literature extensively describes the ingestion by aquatic fauna, the transfer into food webs and the potential action as a vector for toxic compounds or alien microorganisms. Although the scientific community addresses this issue with considerable effort, many questions remain open. In particular, new sources of microplastics have been recently recognized, possibly representing major environmental inputs compared to those previously considered. In addition to the already renowned sources such as the embrittlement of plastic litter and microbeads released from personal care products, microplastic can be released also by washing of synthetic clothes, abrasion of vehicles tyres and from the weathering of different kind of paints. This review tries to exhaustively enumerate all the possible sources of plastic litter that have been identified so far and to report quantitative assessments of their inputs on microplastics pollution to natural environments reported in scientific and grey literature, with an emphasis on surface waters.

Perfluoroalkyl acids in fish of Italian deep lakes: Environmental and human risk assessment.

Determination of 20 PFASs in a fish species (Alosa agone) of commercial interest has been carried out in five Italian subalpine lakes to assess the risk for humans and predators for fish consumption. PFOS still presents the highest concentrations (0.9-16.6ngg-1ww) among the analysed PFASs, in spite of its normative restrictions. PFOS concentrations measured in all lakes, except in Lake Maggiore, are homogeneous with an average of 3.1±1.9ngg-1ww, which could be considered the "anthropogenic background concentration" of PFOS in fish of lakes located in an industrialised and urbanised region but without point sources. In Lake Maggiore, fish concentrations always exceed the EU EQSbiota (9.1ngg-1ww) based on human fish consumption. Considering the effective consumption of fish in this area, an actual risk for fish consumption by humans is not evidenced, while a moderate risk of secondary poisoning for predators is highlighted. PFOA has been detected in significant concentration only in one sample in Lake Maggiore, while long chain PFCAs have been detected without significant differences among the lakes (0.3 to 2.7ngg-1ww). The present study demonstrates that biota monitoring of fish can be used as a valuable tool to classify the quality status of water bodies regarding bioaccumulative PFAAs, even if the water concentrations are close to the reachable detection limits.

Healthy lifestyle promotion in primary schools through the board game Kaledo: a pilot cluster randomized trial.

The board game Kaledo was proven to be effective in improving nutrition knowledge and in modifying dietary behavior in students attending middle and high school. The present pilot study aims to reproduce these results in younger students (7-11 years old) attending primary school. A total of 1313 children from ten schools were recruited to participate in the present study. Participants were randomized into two groups: (1) the treatment group which consisted of playing Kaledo over 20 sessions and (2) the no intervention group. Anthropometric measures were carried out for both groups at baseline (prior to any treatment) and at two follow-up post-assessments (8 and 18 months). All the participants completed a questionnaire concerning physical activity and a 1-week food diary at each assessment. The primary outcomes were (i) BMI z-score, (ii) scores on physical activity, and (iii) scores on a dietary questionnaire. BMI z-score was significantly lower in the treated group compared to the control group at 8 months. Frequency and duration of self-reported physical activity were also significantly augmented in the treated group compared to the control group at both post-assessments. Moreover, a significant increase in the consumption of healthy food and a significant decrease in junk food intake were observed in the treated group. CONCLUSION: The present results confirm the efficacy of Kaledo in younger students in primary schools, and it can be used as a useful nutritional tool for obesity prevention programs in children. What is Known: • Kaledo is a new educational board game to improve nutrition knowledge and to promote a healthy lifestyle. • In two cluster randomized trials conducted in Campania region (Italy), we showed that Kaledo could improve nutrition knowledge and dietary behavior and have a positive effect on the BMI z-score in children with age ranging from 9 to 14 years old attending school. • Kaledo may be used as an effective tool for obesity prevention programs in middle and high school students. What is New: • Investigating the effects of Kaledo on younger primary school children (7-11 year olds), Kaledo could be an effective tool in obesity prevention programs for children as young as 7 years old.

Kaledo, a board game for nutrition education of children and adolescents at school: cluster randomized controlled trial of healthy lifestyle promotion.

UNLABELLED: During childhood and adolescence, a game could be an effective educational tool to learn healthy eating habits. We developed Kaledo, a new board game, to promote nutrition education and to improve dietary behavior. A two-group design with one pre-treatment assessment and two post-treatment assessments was employed. A total of 3,110 subjects (9-19 years old) from 20 schools in Campania, Italy, were included in the trial. In the treated group, the game was introduced each week over 20 consecutive weeks. Control group did not receive any intervention. The primary outcomes were (i) score on the "Adolescent Food Habits Checklist" (AFHC), (ii) scores on a dietary questionnaire, and (iii) BMI z-score. At the first post-assessment (6 months), the treated group obtained significantly higher scores than the control group on the AFHC (14.4 (95 % confidence interval (CI) 14.0 to 14.8) vs 10.9 (95 % CI 10.6 to -11.2); F(1,20) = 72.677; p < 0.001) and on four sections of the dietary questionnaire: "nutrition knowledge" (6.5 (6.4 to 6.6) vs 4.6 (4.5 to 4.7); F(1,16) = 78.763; p < 0.001), "healthy and unhealthy diet and food" (11.2 (11.0 to 11.4) vs 10.4 (10.3 to 10.6); F(1,32) = 21.324; p < 0.001), "food habits" (32.4 (32.0 to 32.8) vs 27.64 (27.3 to 28.0); F(1,26) = 195.039; p < 0.001), and "physical activity" (13.4 (13.2 to 13.7) vs 12.0 (11.8 to 12.6); F(1,20) = 20.765; p < 0.001). Moreover, the treated group had significantly lower BMI z-score with respect to the controls at the first (0.44 (0.42 to 0.46) vs 0.58 (0.56 to 0.59), F(1,18) = 16.584, p = 0.001) and at the second (18 months) (0.34 (0.30 to 0.38) vs 0.58 (0.54 to 0.62), F(1,13) = 7.577; p = 0.017) post-assessments. CONCLUSION: Kaledo improved nutrition knowledge and dietary behavior over 6 months and had a sustained effect on the BMI z-score. Therefore, it may be used as an effective tool in childhood and adolescence obesity prevention programs.

Evaluation of spatial distribution and accumulation of novel brominated flame retardants, HBCD and PBDEs in an Italian subalpine lake using zebra mussel (Dreissena polymorpha).

Because of the reduction in the use of polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD), novel brominated flame retardants (NBFRs), including 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ethane (DBDPE), hexabromobenzene (HBB), and pentabromoethylbenzene (PBEB), started to be marketed as alternatives to the banned formulations. In this study, the spatial distribution and accumulation of NBFRs, PBDEs, and HBCD in the biota have been investigated in the littoral compartment of a large and deep subalpine lake (Lake Maggiore, Northern Italy), using zebra mussel Dreissena polymorpha and roach (Rutilus rutilus) as bioindicators. To our knowledge, this is the first study reporting the contamination of NBFRs in the freshwater invertebrate D. polymorpha. Contamination of zebra mussel due to PBEB, HBB, and BTBPE was low, ranging from 0.9 to 2.9 ng/g lipid weight, from 1.1 to 2.9 ng/g l.w., and from 3.5 to 9.5 ng/g l.w., respectively. PBEB and BTBPE in roach were always below the detection limit, while the contamination of HBB ranged from < limits of detection (LOD) to 1.74 ng/g l.w., indicating a weak contamination. DBDPE was < LOD in all the considered biological samples. Finally, HBCD was detected in all organic tissues with mean concentrations up to 74.4 ng/g l.w. PBDE results, supported by principal component analysis elaboration, suggested a possible contamination due to the congeners composing the penta- and deca-BDE technical formulations, which are present in the Lake Maggiore basin. The biomagnification factor values showed that tetra- and penta-BDE biomagnified, while octa-, nona-, and deca-BDE were still bioavailable and detectable in the fish muscles, but they do not biomagnified. Considering the other BFRs, only HBCD showed a moderate biomagnification potential.