Chemometric methods for studying the relationships between trace elements in laryngeal cancer and healthy tissues.

A quick and reliable method for the evaluation and classification of two types of tissues is presented. Several chemometric methods were applied to evaluate multivariate data of the tissue samples with respect to the content of trace elements. The content of Pb, Al, Zn, Cd, Cu, Ni and Co was determined in samples of healthy and cancerous tissue obtained from 26 patients. Determination was done at milligram/kilogram level with inductively coupled plasma optical emission spectrometry (ICP-OES) and atomic absorption spectroscopy (AAS) techniques. Contents of trace metals in studied tissues are not normally distributed; however, normal distribution was confirmed for log values. There is a statistically significant difference in the content of Zn, Cd, Cu and Al (p<0.01) and Ni and Co (p<0.05) when healthy tissue is compared to cancerous one. Correlation between contents of trace elements for studied tissues was positive; the highest was found between Zn and Cu. A chemometric methodology seems to be a promising tool for classifications of the tissue samples.

Pleomorphic fibroma of tendon sheath.

We report an example of fibroma of tendon sheath with pleomorphic bizarre cells without mitoses. The tumor appears to be morphologically identical to a similar tumor in the skin and represents yet another example of a benign soft-tissue neoplasm with disturbing morphological features.

Felty's and Sjögren's syndromes and hypertrophic obstructive cardiomyopathy (case report).

Our findings refer to a female patient with Felty's and Sjögren's syndromes and hypertrophic obstructive cardiomyopathy. The rheumatoid granuloma found in the left ventricle at autopsy and the absence of other conditions which may be manifested by hypertrophic obstructive cardiomyopathy suggest that the case studied, probably represents an extraarticular manifestation of Felty's and Sjögren's syndromes.

[Amniotic fluid embolism in multiple pregnancy in the second trimester of gestation]. / Embolija amnijskom tekucinom kod viseplodne trudnoce u drugom tromjesecju gestacije.

A rare case of amniotic fluid embolism in the 26-th week of pregnancy is presented. After the McDonald operation for the repair of the incompetent cervix, a premature rupture of the membranes occurred. After a five-day period of amniorrhea an elevation of the body temperature over 39 degrees C was observed, as well as respiratory distress, cyanosis, and coma. It ended with the patient's death. The pathomorphological finding demonstrated the amniotic fluid embolism of the lung and brain arterioles.

Studies on the postnatal growth of the rat adrenal cortex.

In the male rat the volumes of the several zones of the adrenal cortex were measured using a point-counting technique at various ages. The resulting growth curves were fitted by the Gompertz method, and the specific growth rate calculated for each zone at 14 days of age. Also at 14 days the flash labelling index was measured in contiguous bands each three cells in thickness across the cortex; maximum indices were found in the outer cortical layers. Calculations of the birth rate for each zona were compared with the growth rates. In the zona glomerulosa, the birth rate exceeded the growth rate, giving a cell migration rate of 4 cells/1000 cells/hour. In the zona reticularis there was a net cell gain of 5 cells/1000 cells/hour; in this zone, for every 100 cells born by cell division, nearly 2000 were added by cell migration. The results support the migration hypothesis of adrenocortical cytogenesis.