1.
Bioorg Khim
; 18(2): 210-6, 1992 Feb.
Article
in Russian
| MEDLINE
| ID: mdl-1534987
ABSTRACT
In search for sorbents (silica gels, styrene-divinylbenzene copolymers), for immobilization of some restriction endonucleases, derivatives of trityl-containing silochroms are shown to bind EcoRI, PaeI and LplI endonucleases with the retention of 10-20, 60-70 and 40-60% activity, respectively. The immobilized restriction endonucleases have the unchanged substrate specificity, can be used several times and are stable at storage. Tritylaminopropylsilochrom is suggested to be the sorbent of choice.