ABSTRACT
Gravitactic protozoa offer advantages in studying how the gravity stimulus is perceived on the cellular level. By means of a slow rotating centrifuge microscope in space the acceleration thresholds for gravitaxis of Loxodes striatus and Paramecium biaurelia were determined: < or = 0.15 x g for Loxodes and 0.3 x g for Paramecium, indicating different sensitivities of these species. Neutral-buoyant densities of immobilized cells were determined using media of different densities, revealing densities of 1.03 to 1.035 g/cm3 for Loxodes and 1.04 g/cm3 to 1.045 g/cm3 for Paramecium. Behavioral studies revealed that gravitaxis of Loxodes persisted independent of the density of the medium. In contrast, negative gravitaxis of Paramecium was no longer measurable if the density of the medium approached the density of the cell. The results suggest that in the case of Loxodes gravity is perceived by an intracellular receptor and, in the case of Paramecium by its own mass via the pressure on the lower cell membrane.
Subject(s)
Ciliophora/physiology , Gravity Sensing/physiology , Locomotion/physiology , Paramecium/physiology , Animals , Centrifugation , Culture Media , Hypergravity , Orientation/physiology , Rotation , Space Flight , Swimming/physiology , WeightlessnessABSTRACT
Behavioural responses to different accelerations below 1 g and up to 5 g were investigated in Paramecium biaurelia by using a centrifuge microscope on Earth and in space during a recent space flight. Increased stimulation (hypergravity) enhanced the negative gravitactic and the gravikinetic responses in Paramecium biaurelia within seconds. Cells did not adapt to altered gravitational conditions. Repetitive stimulation did not change the graviresponses. The minimum acceleration found to induce gravitaxis was between 0.16 and 0.3 g.
Subject(s)
Gravity Sensing/physiology , Hypergravity , Paramecium , Rotation , Space Flight , Weightlessness , Acceleration , Animals , Centrifugation/instrumentation , Gravitation , Image Processing, Computer-Assisted , Microscopy/instrumentation , Motor Activity , SwimmingABSTRACT
The gravitactic ciliates Paramecium and Loxodes were cultivated for 15 days in space during the IML-2 spacelab mission. At dedicated times their behavioral responses to different accelerations between 10(-3) x g and 1.5 x g were investigated by using a slow rotating centrifuge microscope (NIZEMI). The threshold for gravitaxis of Paramecium was found to be at > 0.16 x g and < or = 0.3 x g. No adaptation of Paramecium to the conditions of weightlessness was observed over the duration of 15 days. Loxodes showed no graviresponses to increasing accelerations, though it demonstrated gravitaxis after return to earth.
Subject(s)
Acceleration , Ciliophora/physiology , Gravity Sensing/physiology , Paramecium/physiology , Space Flight , Weightlessness , Animals , Cell Culture Techniques/methods , Centrifugation , Image Processing, Computer-Assisted , Movement , SwimmingABSTRACT
Behavioural responses to different accelerations below 1 g and up to 5 g were investigated in Paramecium biaurelia by using a centrifuge microscope on Earth and in space during a recent space flight. Increased stimulation (hypergravity) enhanced the negative gravitactic and the gravikinetic responses in Paramecium biaurelia within seconds. Cells did not adapt to altered gravitational conditions. Repetitive stimulation did not change the graviresponses. The minimum acceleration found to induce gravitaxis was between 0.16 and 0.3 g.
