Influence of Rectal Ozone Application on the Intensity of Free Radical Destruction of Lipids and Intestinal Proteins in the Dynamics of Experimental Colitis.

We studied clinical status, content of products of LPO, and oxidative modification of proteins (OMP) in the lesion focus of the intestine in experimental colitis under conditions of rectal administration of ozone. Experimental colitis was simulated by two-stage administration of oxazolone; rectal insufflation of ozone in the ozone-oxygen mixture was performed daily. The disease activity index (DAI), the content of calprotectin in the feces, and LPO and OMP products in the intestinal homogenate were assessed. On days 2, 4, and 6 of the pathological process, DAI, concentration of calprotectin in feces, content of primary, secondary, and end-products of LPO in the heptane and isopropanol phases, and content of primary and secondary OMP products progressively increases. Under conditions of ozone application, DAI, concentration of calprotectin in feces, the levels of heptane- and isopropanol-soluble primary, secondary, and end-products of LPO, and the level of primary and secondary products of OMP decreased on days 4 and 6; the level of isopropanol-soluble primary, secondary, end-products of LPO increased on day 2 of experimental colitis. The severity of clinical manifestations weakens as the content of LPO and OMP products in the colon decreases on days 4 and 6 of observation.

Effect of Vitamin D3 in Composition of Original Rectal Suppositories on Parameter of Protein Oxidative Modification in Large Intestine in Experimental Ulcerative Colitis.

The effect of vitamin D3 in the composition of original rectal suppositories on the content of products of oxidative modification of proteins in mucous membrane of the large intestine was studied in rats with experimental ulcerative colitis provoked by a two-stage administration of 3% oxazolone. The rectal suppositories with vitamin D3 (1500 IU) were administered every 12 h during 5 days. Condition of the rats was assessed according to disease activity index (DAI), while the content of oxidative modification products of proteins in the homogenate of the mucous membrane was assayed with extraction-spectrophotometric method in the lesion focus of large intestine. DAI increased during entire observation period of ulcerative colitis, which correlated with the level of products of spontaneous and induced oxidative modification of proteins in mucous membrane of the colon. The study examined the pharmaceutical and technological features of novel rectal suppositories of original composition weighing 300 mg, which are based on polyethylene glycol supplemented with aqueous solution of vitamin D3 (10%). The use of rectal suppositories with vitamin D3 reduced DAI and inhibited the oxidative modification of proteins.

[Changes in the erythroblast islands of the bone marrow in animals under combined heat amd muscle loads]. / Ob izmenenii éritroblasticheskikh ostrovkov kostnogo mozga u zhivotnykh pri sochetanii teplovykh i myshechnykh nagruzok.

Quantitative composition of the erythroblast islands was studied during exposure of rats to heat and physical exercise. The exercise activated the differentiation and proliferation of erythroid cells in the erythroblast islands. The combination of heat exposure and physical exercise inhibited these processes.

[The characteristics of porphyrin metabolism and of the erythron indices under the joint action of heat and physical loading on the body in rats]. / Ob osobennostiakh porfirinovogo obmena i pokazatelei éritrona v usloviiakh sochetannogo vozdeistviia teplovoi i fizicheskoi nagruzok na organizm krys.

Exercise was shown to activate the proliferation of red cells and their hemoglobin synthesis. Combination of heat exposure and physical exercise inhibited these processes.

[The interrelation of the degree of animal training, porphyrin metabolism and erythron indices]. / O vzaimosviazi trenirovannosti zhivotnykh, porfirinovogo obmena i pokazatelei éritrona.

Parameters of erythron and porphyrin exchange were studied during exercise. The long-term exercise (a few weeks) intensifies the synthesis of heme and hemoglobin before activation of the red cell proliferation.