ABSTRACT
Results of surgical treatment of 479 patients with deep burns of the upper extremities on the area from 0.5 to 60% of body surface with the lesion of shoulder (398), elbow (407), radiocarpal (371) joints and hand (423) area. Early or delayed necrectomy (28.6%) with following skin grafting was performed at hospitalization of patients in early period. At IV-degree burns plastic reconstruction with skin-subcutaneous flaps was used: local (22), combined (16), pedicle flap and Filatov's graft (29), flaps with axial circulation (8) and on microsurgical anastomoses. Active surgical policy resulted in recovery of the function of the shoulder joints in 73.7%, elbow joints--in 75.15%, radiocarpal joints--in 83.4% and of hand in 50.2% cases.
Subject(s)
Burns/rehabilitation , Hand Injuries/rehabilitation , Plastic Surgery Procedures/methods , Wound Healing/physiology , Adolescent , Adult , Aged , Aged, 80 and over , Burns/pathology , Burns/surgery , Follow-Up Studies , Hand Injuries/pathology , Hand Injuries/surgery , Humans , Male , Middle Aged , Prognosis , Skin Transplantation , Surgical Flaps/blood supply , Trauma Severity IndicesABSTRACT
Aiming to create an adequate model for investigation of the molecular mechanisms involved in transcriptional regulation by steroid hormones, a number of cell lines carrying bovine papilloma virus (BPV) based constructs containing the mouse mammary tumor virus long terminal repeat (LTR) were established (Ostrowski et al., Mol. Cell. Biol. 3, 2945-2957, 1983). However, all our attempts to extract from the cells such minichromosomes as nucleoprotein complexes using a method previously described (Ostrowski, Nucleic Acids Res. 15, 6957-6971, 1987) failed. Here, we show that this failure was attributable to DNA rearrangements in most of the cell lines, resulting in the integration of the BPV-based constructs into the host cell genome. We have identified two cell lines where the constructs are episomal. Micrococcal nuclease digestion of the nuclei demonstrated the presence of nucleosomes positioned over the episomal MMTV LTR. We managed to optimize conditions for preparation of nuclei and minichromosomes, which allowed extraction of approximately 40% of the minichromosomes, most of them being in circular superhelical form. Our data show clearly that the main factor preventing the release of minichromosomes from the nuclei is the presence of polyamines in the cell lysis buffer. The organization of MMTV promoter chromatin was unaffected by the extraction procedure, suggesting that these minichromosomes could be valuable templates for in vitro transcription studies and to identify proteins involved in chromatin remodeling during transcription.
Subject(s)
Bovine papillomavirus 1/chemistry , Chromatin/chemistry , Plasmids/chemistry , Animals , Bovine papillomavirus 1/genetics , Buffers , Cattle , Cell Line , Chromatin/isolation & purification , DNA, Recombinant/chemistry , DNA, Recombinant/isolation & purification , Genetic Techniques , Micrococcal Nuclease/chemistry , Nucleosomes/chemistry , Plasmids/isolation & purification , Polyamines , Terminal Repeat SequencesABSTRACT
1.897 patients with upper gastrointestinal bleeding (UGIB) were studied and the cause was diagnosed in 1.756 (92.6
). The most frequently found pathology was the acute bleeding gastropathy (24.5
). Considering the gastric duodenal and anastomotic ulcer as a whole, in 47.5
of the cases the ulcer was observed. Both pathologies together make out that 72 out of 100 patients with UGIB have bleeding due to an ulcer or gastritis. It comes out that the low incidence of neoplasy as a consequence of UGIB and that the 31
of the diagnosed pathology could not have been diagnosed by X-ray.
ABSTRACT
1.897 patients with upper gastrointestinal bleeding (UGIB) were studied and the cause was diagnosed in 1.756 (92.6
). The most frequently found pathology was the acute bleeding gastropathy (24.5
). Considering the gastric duodenal and anastomotic ulcer as a whole, in 47.5
of the cases the ulcer was observed. Both pathologies together make out that 72 out of 100 patients with UGIB have bleeding due to an ulcer or gastritis. It comes out that the low incidence of neoplasy as a consequence of UGIB and that the 31
of the diagnosed pathology could not have been diagnosed by X-ray.
