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Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity.

Vorobieva, E I; Meringova, L F; Leontieva, G F; Grabovskaya, K B; Suvorov, A N.

Folia Microbiol (Praha) ; 50(2): 172-6, 2005.

Article in English | MEDLINE | ID: mdl-16110924

ABSTRACT

Group B streptococcal (GBS) gene encoding the putative lipoprotein and adherence factor ScaAB was cloned and expressed in E. coli. Recombinant ScaAB protein was isolated. Signal sequence of ScaAB was found to be cleaved in the E. coli host. ScaAB recombinant protein was immunogenic in mice and antibodies against this protein were discovered in mice sera after GBS infection. The perspectives of the use of ScaAB protein in GBS vaccine are discussed.

Subject(s)

Bacterial Proteins/immunology , Membrane Proteins/immunology , Recombinant Proteins/immunology , Streptococcal Infections/prevention & control , Streptococcus agalactiae/immunology , Amino Acid Sequence , Animals , Antibodies, Bacterial/blood , Bacterial Adhesion , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Immunization , Membrane Proteins/chemistry , Membrane Proteins/genetics , Membrane Proteins/metabolism , Mice , Molecular Sequence Data , Recombinant Proteins/chemistry , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Streptococcal Infections/microbiology , Streptococcal Infections/mortality , Streptococcus agalactiae/isolation & purification , Streptococcus agalactiae/pathogenicity

ABSTRACT

Subject(s)

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